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3:59 Concerning the RP motility, how is it really done? Per the field at 3:59, according to my observation, about 15 RP sperms are not seen at once. At some time, less than 10. So how did we arrive at 35?
Previously it was easier as we had to differentiate between three levels of motility active, , sluggish, and immotile, which accurately assessed. However, it became a challenge when active was split into rapid and slow. Going back to the definition in 1:49 with a bit of practice as in our lab will help overcome the difficulty
The explanation in this video is very clear, but it also contains a major mistake. In the equation at 0:50, the sigma means that you have to add up those values across ALL possible values of i and j. So not only do you need to do the comparison between sequence 1 and 10, for example, but you also need to do the reciprocal comparison of sequence 10 and sequence 1. Both of those comparisons, of course, give the same value. So if you decide to take the logical shortcut (as in this video) and only do each unique comparison once, you need to multiply your final value by 2 to account for the missing duplicate comparisons. Example: Suppose you are just comparing two sequences that differ at every nucleotide and each make up half the population. Using the system in this video, you would get a value of pi equal to (2/1)(1/2)(1/2)(1). That comes out to 0.5 when it should really be 1.0. The problem is the missing factor of 2. Wikipedia has the correct formulas: en.wikipedia.org/wiki/Nucleotide_diversity
عند هاي الخطوة 3:17 مابيرضى يعمل ألاين وبيعطيني تنبيه Failed to load fast file. Data parsing error. Expected sequence but none found. Access violation. كيف ممكن احلها😢
Hello sir I am facing problem in making colour haplotype networking. Sir there is a error "alignment sequences are not same as in trait "but sir i have copied it from nexus file only sequences are same, Ids are same why i am not able to execute it forward. Please let me know sir