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From the following data calculate the mean generation time. At the beginning of exponential growth when the t=0 Initial cell concentration=2500per ml .At time t=8hrs cell concentration=10000per ml
I got everything, but I have one question. When we take 1ml from each tube and transfer it to the next, the concentration of all of them decreases to one 1ml, but the last tube will have 1 ml more bcz it wont be tranfered to the next tube. so how do we bring its volume equal to the rest of the tubes?
Concentration does not depend on volume. Example: Prepare a 1000 mL solution of NaCl with 1mg/mL concentration. If you transfer 500 mL to another container, its concentration will still be 1mg/mL only. Hope you understand.
If I were to use vial 3 and 4, how will I standardised / average them? From the reading, they are 2,600,000 CFU to 3,500,000 CFU respectively. Will the average be 3,050,000 CFU?
@@MicrobiologyMantra thank you. Yes, both have countable CFUs, as per your illustration. Though the computed ranges are wide, that's why I am seeking guidance.
