All things mycology and mushroom. If you would like to support the channel, please visit my shop and these channels: sporeswaps.com/vendors/dr-eds-spore-shop/ www.buymeacoffee.com/edwardgrand Edward Grand RU-vid: www.youtube.com/@edwardgrand The Clamp Connection RU-vid: www.youtube.com/@TheClampConnection Serene Mycology RU-vid: www.youtube.com/@SereneMycology Satori Myco RU-vid: www.youtube.com/@SatoriMyco Instagram: instagram.com/edward.grand/ Facebook: facebook.com/edwardgrand
I'm a pro photographer as I told you before.. Your cell camera has a fixed aperture, it doesn't change. However, the aperture maybe different between the multiple sensors in the camera array. What I do to get better macros from my S21 Ultra is I put the phone on a tripod, switch to the 3x telephoto zoom sensor and frame the shot I want via moving the subject or the tripod then snap the photo with a 10sec timer or my galaxy watch's remote control. If your phone has a camera array, try to use the telephoto zoom (do not use digital zoom!) and be sure to throw some good light on the subject. Darken the room, set subject on a well-lit table and snap at an oblique angle. Should turn out well. If you have any a/v questions feel free to reach out, Ed.
@ETMIRAGE thank you for the advice. I just moved my ring lights and backed up my tripod. Think it'll do a bit better to add some contrast. Some of my photos look very 2 dimensional.
@@edwardgrand yeah man, the phone camera sensors are just too small to get the depth of field desired most of the time. The f-stop or aperture number signifies how much light is getting into the sensor, or how open the camera's iris is. So an aperture of f22 would make the iris of the camera (aperture) a small pinhole which makes everything in frame sharp and in focus. We don't want that, we're not shooting landscapes on sunny days. We need to drop that aperture number, open the iris all the way to let in as much light as possible to get a faster shutter (faster shutter speeds help with making sharp photos because there's less time for a shaky hand or even a shutter vibration from the camera itself to ruin the photo), along with giving us the desired depth of field. You'll notice if you look around at the prices of lenses, the lower the f number, the more expensive they get.. everyone wants that look. I know things cost money but if you're interested in looking for a better camera, check out the Panasonic G85. The 12mm-65mm kit lens is excellent for video, camera shoots up to 4k 30fps & 1080p 60fps. Manual lenses are cheap, the camera platform is still the absolute best value for a 4k mirrorless (no record time limits either, only limited by SD card space and battery life (battery life until you get a dummy battery that plugs into a wall outlet or USB power bank charger)). Get the one with the 12-65mm kit lens and grab a manual Panasonic 25mm and you'll be off to the races. You could get that camera, the extra 25mm f1.7, a dummy battery and a forward/reverse facing Deity D4 Duo camera mic (this would be great for your pov vids for when you're holding the camera and speaking into it, the mic is pointing at your face) for just under $950 US new. ..they've been making this camera for almost ten years now, you can find them second or third hand in decent condition for much cheaper than new. I actually bought mine second hand and it hasn't skipped a beat. Sorry for the novel, lol. Just trying to teach the teacher!
Hello Ed, i tried to reach you threw Spore Swap and sent this same question, but don't think it went through. So I hope this is not coming at you twice. But if you don't mind my ignorance to the facts of how things work. and if you have the time, The question I asked was as follows: Hello Ed, I have a question I was thinking about, but did not want to try it without first asking someone if it is even plausible. Let's say I took the fruits of a grow and dried them out and ground them into a powder, then soaked the Powder mushrooms in water for a month. (Assuming the active ingredients would leach into the water). If I then used that water for grain hydration of the next batch of Spawn, or used it to hydrate the next batch of substrate for the next grow. Would it ADD anything to the potency of that next grows fruits?
It would not. Waste of fruit. If you want to experiment that way, tryptophan supplementation would be better. and cheaper. My Facebook and IG are in the descriiption.
Do what @jonb said. Make sure the notification bell is all black too. You might also have to go into you settings. I somehow managed to turn off notifications for some channels at some point in my settings (the cog/gear looking thing).
Great video ed. I have 6 month LC in a syringe( master clone) , if I extend the LC would I get the same potency ? Is the mycelium just older not experienced Senescence
The age of the LC won't have any effect on the potency of the final mushrooms. The time it has spent dormant in the syringe is not enough for it to senesce. I would check in on agar to make sure it's not contaminated and still show vigorous growth. You can then use that fresh plate to make spawn or inoculate new LC. If you just want to extend it by shooting it into fresh LC, it might be hard to know if the growth is sufficiently vigorous or check for contams.
We could really benefit from a video explaining the whole chasing rhizo growth on agar thing. To me it would seem like the morphology is just a response to nutrient availability, but others swear that achieving rhizo on agar will make it rhizo in grain or whatever next medium. This seems wrong. I know you’ve touched on it, but really this is a thing that needs debunked or proven in this community.
I really wish I could give some definitive guidelines on that topics. Unfortunately, most of my experience is undocumented anecdotes from years of FAFO experimentation and unstable growing/lab conditions. I don't think I can add anything more to the discussion than most advanced growers could. I know it is one of the Dunning-Kruger situations though. For the first few years or cultivation, you think you've got it all figured out. Then you just hit this wall where you realize you don't understand shit about fungal growth, or anything else.
I got introduced to CSTR and bio-reactors back in college. I wish I would have known what they could have been used for back then. I would have really given a lot more of fucks to all that mass/energy balance shit. And logistics. I had whole labs on distillation, but those tight ass engineer profs never mentioned the alcohol industry. Fuck me. I'd be a lot richer now if I would have stuck with the engineering, but I'd probably also have 2 mortgages, 3 kids, and 2 divorces under my belt!
Hey Ed, i am doing a lot of agar work and want to advance into incorporating microscopy into my workflow, for learning reasons and maybe eventual mon-mon cross attempts. My intuition tells me that microscope work without a flow hood would be a horrible idea. Is that a correct assumption? Also, I see that you and some others seem to work in vertical flow hoods, some horizontal. Again intuitively it would seem like a vertical hood could cause turbulence as it hits the work surface, and potentially take breath/spit and propel it downward. Would a horizontal would be more sterile? Is the vertical just preferable ergonomically? What I’m getting at is, which type should I invest in as I have to select only one. Thank you so much for being open to questions and educating us! 💚
Either put the scope in front of the FFU or take samples and move to the scope area (that is what I do). It is personal preference for vertical or horizontal. Everyone has a different workflow and budget. I would get a horizontal FFU. Cheaper, easier to move, set-up and maintain. Thank you for watching and commenting. Good questions.
Thank you. I've learned to be more patient over the last couple years. I've still got some work to do though. I get triggered like everyone else. LOL Thank you for watching and commenting.
Thanks for the Peterson reference! I found several of his papers on Google scholar. I've got some reading for the next few days, I'm really starting to enjoy the academic papers, always loved to read Informative literature, but never read very many published papers. Thanks again!
@@edwardgrand 😁I feel that but with electrical troubleshooting manuals. I think about 30 different machine service manuals are permanently burned into my retinas. Lol most of my adult life has been spent chasing down plc programmer problems
No shit I thought today was Friday too 🤦 I'm really slacking and I even missed your live stream.. well at least I got you to listen to now while sitting in the flow of purified air. Have a great night Ed!
missed it again! ed! one of these days you’ll have to create a streaming schedule so we know when to tune in. maybe even just the day before or a couple days before. have you ever thought of making a discord group? that would be an awesome way to connect with you. thanks for everything you do. we learn so much.
I won't likely make a schedule. That seems too much like a job. All my social media is in the description. You can contact me there. Thanks for watching.
That is about right. I change it all the time because my coir and verm sources are a bit inconsistent. I have also adjusted the amount of grain I put in each bag, so I get better colonization and more bags per PC run. So nothing is really consistent. I am not after yields, so no problem for me. I just want pretty mushrooms.
Hey Edward! So as soon as I got into my college, I realized I really loved the microscopy and breeding side of things. I’m an engineer and I like to know how things work from starts to end. Yours and others RU-vid videos on these more technical subjects are highly appreciated. I see there are some good resources in the description. Is there something close to resembling a comprehensive textbook on genetics and breeding? I’ve been eyeballing the radical mycology book, but I would like to ask your opinion on recommendations.
Radical Mycology is great, but not much on breeding. The Chang and Raper articles are good. I would go through the first reference. Edible and medicinal fungi breeding techniques, a review: Current status and future prospects. Yating Dong www.sciencedirect.com/science/article/pii/S2665927122001435
Fan Filter Unit. They are cheaper, lighter versions of laminar flow hoods. They are meant for the ceiling of clean rooms, but we turn then sideways and use them as 'horizontal' flow hoods.
I want some basmati with beans dumped on it, with cayenne and some masala powder, eat it before bed drop 3 Deuce's before heading to the factory, or red lentils, get shredded eating like this with whole foods, treat processed food like the plague, gotta aim for the antiinflammatory foods. Rice is a dirty crop but I rinse the hell out mine, my mangled bones been feeling good compared to how they used to, getting older a bitcch we gotta eat to live and stack the cards in our favor, prevention is key, peace
I'm the guy you talked to about the IP agar problems & condiment cups. So I figured out the issue with contamed agar besides the 30min PC time, you're gonna love this.. I bought a larger diameter trivet for the IP. It was only about 1/8th inch taller than the smaller one but the loose lid of my media bottle was just contacting the bottom side of the IP lid.. No steam was getting into the agar!! Figured that out after we talked and I just poured a new run of condiment cups yesterday. I also let it cool down to about 110F before pouring, condensation was noticeably better. I'll wait until Thursday to inspect the cups but I have a much better feeling about this run. This issue started exactly when I got that new trivet. Even though I had successful runs before at 30min, I'll follow your direction on th 1hr PC time. Now, if I can figure out how to get larger fruits instead of mats of 2in tall pins we'll be rolling. Thanks again, Ed!
Glad you figured it out. The small fruit might be them running out of moisture from the substrate. Try adding more substrate to the same amount of spawn or cutting down on the spawn for the same final total volume.
@@edwardgrand Right on. Mine were noticeably thinner than your shoeboxes, they're about 2in. I think my moisture levels are good due to the weight but I went ahead and got all the little stuff off and dunked them for about 45min and drained. I'll give them another week or so for the second flush (if there's a second one) and move on from there. One more question, you said the agar will absorb any water droplets, i.e. condesation droplets (tiny, not moving around the rim of the agar) that fall or a drop from LC.. will that droplet leave a spot on the agar where it was? I see spots where droplets could have landed but there's a dullness to them, like water spots on a car's paint. ..they don't appear to be growing. Sorry, I'm autisticly paranoid about contam at this point, lol
@edwardgrand buddy... I just tried 3gees (🍋tek) of my wild E. Texas ABCs... They're exactly what they should be, exactly how I remember them. Everything is good all the way around. AGAIN, you da man!
QQQ I used to cook agar for 1 hour but I've started using corn starch broth once in a while instead of potato starch just to give the myc something new to eat on the plate but ive noticed that 1 hour wont sterilize all of it at 15 psi. IDK if the corn meal I'm boiling is very dirty or if it's just super nutritious and causing a few bad plates out of every 50 so I started going 90 min at 15 psi and have a noticeable difference. What are your thoughts on it? Could it maybe be the plates? I need to do a controlled experiment using a different brand of plates I guess and multiple cook times
@@edwardgrand I agree, I think il pour 100 plates tonight and set up two diff media and two diff plates, 25 of each and see what happens. If anything il have the next few weeks plates to use
@@edwardgrand problem solved with the bad plates. Pouring 2 types of media on two different plates, it was one brand of plates. I also noticed why, the lid on one brand had a very short lip so as I lifted the stack, the bottom of the stack which are the hardest to balance I caught myself on camera brushing the edge of the dish on the short lip plates with the edge of my finger. Didn't happen with the other plates having the larger lip on the lid and only the bottom 2 plates. So simple, I'm very glad I didn't go making any changes to my media. Now I know not to buy those cheaper plates. Thank you for all of the knowledge you put out there. Before I started listening to you I probably wouldnt have reacted and solved this the way I did. You are a pillar in the mycology community and I'm forever grateful.
I missed the end of the live but talking about grains. Lol I also use oats from the horse feed section of rural king. I found bird seed that is only Milo milet and wheat berries, most bird seed is a mix mash of a bunch of shitty grains. I was stoked to find the right mix. It works really well and has the same cook time as oats so I mix them a lot. Hell sometimes when I am running out I will mix the two and then cook popcorn separate just to make up the weight I need. My first few years I used rye berries and then realized I was wasting money, rye berries aren't any better than rice or oats so why spend the money....... But popcorn is my favorite, just expensive at 0.90 cents a pound
yeah, I don't like wheat berries. Thanks for sharing the information my friend. that is really cool. I think there is a rural king in Chambersburg. It is like an hour from where I live I Pa. I am going to check at some of the privately owned places nearer to me. The next closest is in like Mattoon Illinois...lol
WHAT UP Ed!? soooo can u tell me if a strain is heat tolerant does that mean they can be incubated and fruited in the heat or just fruit .. is incubation pretty similar across the board or differs just as much as fruiting conditions??
