Filtrous is a California based Lab Consumables company with strong ties to the Analytical Testing community. Our mission is to provide excellent products and education to our customer base on all things related to analytical testing.
Hey there, yes we currently do have some on our website under Cell Culture Flasks. You can search on our website or ask our customer support team for help if you need it!
Where can i get 5 micron syringe filters for printer pigment ink on the cheap? Should it be nylon or the poly-something-something stuff? (Name escapes me)
@Filtrous make a YT short of the ketchup filtration and also include the demonstration in this video here. Left us hanging with a novel claim and did not demonstrate it.
so what goes in the wells, how is the layout if we have standards and a reaction mix? Is it, reaction mix in all wells, and then in respective wells: cDNA of target genes, cDNA of housekeeping gene and cDNA of standards? i am so confused
Do not follow this video. Always follow the column manufacturers manual and call their technical support if you have specific application questions. Anyone who would tell you to reverse flow through a HPLC column is basically telling you to piss way $1500. Source: I'm a field service engineer for Thermo Fisher and I work in IC/LC chromatography.
Hey Daniel, this is for a reverse phase column and this is in the column care guide that comes with the column. Yes, you are right that this does not apply to all columns but it does apply to this column as it is reverse phase.
Hey, your videos amazing and very helpful, please can you make a video about how we do the analysis to the result after the pcr done by the thermocycler (biorad)...🙂
What’s the point of keeping the pcr plate on ice? Theres nothing in the mm that degrades at RT and most enzymes have hot start mechanisms to inhibit activity at RT
You would be right most of the time. Some enzymes out there function at lower temps like the NEAR enzyme. Also, there are thermodynamics to all reactions and colder temps limit activity even if it's small. It's a general good practice so you can maximize sensitivity and consistency of your assay.
@@Filtrous interesting! I did not know about NEAR PCR and it sounds interesting. There’s really 0 activity in hot-start enzymes mixes at RT but I get what you are trying to say
This is a good general usage recommendation, but what about for specialty columns? For instance, I sometimes use phenyl columns or HILIC columns. About 80% of what I analyze uses either C8 or C18 Reverse-phase columns, and I would definitely employ this cleaning method, but is there a better cleaning format for the lesser used types of columns?
