You haven't been eating insects, you've been eating an extract of carminic acid that came from insects. That's not the same thing. If you make it with a yeast you're still eating the same thing you were before.
Great video! I'm a student and I don't have a lot of experience with yeast, and methods to transform in yeast. So I was wondering if this method is up-to-date (Since I noticed that the article was published in 2007). Are there also any other methods to make yeast cells competent (e.g. electroporation)?
Hi! I had a few questions about the experiment as I am struggling with it at the moment. Should I post it up here on the comment section? Or, could I have an email to write to you at? Such a super informative video this is!
Super informative video! You mentioned that you can use transformations to delete DNA, how would that be done? I've only seen it be used to introduce exogenous DNA into a cell. Thanks!
Hi Kelly. Glad you liked the video. S. cerevisiae has a very high natural rate of homologous recombination, that means any introduced DNA fragment that comprises atleast 15 nucleotides of identical (homologous) sequence to the intrinsic DNA (e.g. a chromosome) can recombine and fuse into one DNA molecule by a natural DNA repair mechanism. Gene deletion can also be considered "an introduction of exogenous DNA" because we actually replace the targeted gene with a selection marker: Gene deletion (somtimes referred to a knock out) can be performed by introducing an exogenous piece of DNA that comprises segments identical to the 3' and 5' ends of the target gene sorrounding a selection marker gene. This way the exogenous recombinant DNA fragment can recombine with both the 3' and 5' ends of the gene, in the proces replacing the gene with the selection marker gene. Another method for deleting a handful of nucleotides, disrupting a functional gene, is the CRISPR/Cas9 system introducing a double stranded break in the middle of your target gene followed by non-homologous end-joining (unspecific repair)
@@esbjerg_laboratory2958 Gotcha! thanks so much! So if I want to do a chromosomal deletion/large sections of DNA via transformation, the procedure would be the same as any other transformation, except the exogenous DNA I would introduce would the 3' & 5' ends of the chromosome/DNA chunk?
