@@tawseefalaff9976 I typically chill it for this protocol, but if it's at room temperature then you can likely put it in the freezer for 1 or 2 minutes longer.
Is there a need to (pre)mordant, or is there enough tannins in the lichen? If there is a need for premordant, what would give the best effect for purple?
Gorgeous! My MIL was cleaning graves on Memorial Day this year and i asked her to save any lichen she found. I was expecting some of the xanthoria lichens because she described it as bright yellow-green, but instead she brought back nearly a quart of this! Looking forward to trying it out.
After a decent windstorm, I was able to collect enough lichen to fill a 1 L jar, which then provided dye for about 400 or 500 g of wool. It will take time, but the lichen gives more pigment than expected!
Last summer, I made this according to your directions and it worked great! Trying it again and after three weeks I have what looks like grape juice colored solution. My question is: How do I know when it's ready?
Awesome! I think the longer you let it go, the deeper the color for a given amount of liquid. I'm also not sure at which point the lichen is exhausted.
It's just being used for sugars and will be autoclaved, so it doesn't matter. However, I use dry bacteriological malt extract which is presumably non-diastatic due to heat treatment.
I'm pouring in a still air box. Can I leave the lids off the same way until the agar cools or will there be a risk of contamination? I don't have a flow hood.
I have looked everywhere for a "soup to nuts" method of using lichen as a die and you have done it! I also live on the east coast and I thought that the lichen that I had collected was the common Green Shield (Flavoparmelia caperata) but your instructions on using a drop of bleach verified what I had initially thought was Punctelia rudecta. Yay! Purples!!! I love your step by step directions from harvesting, removal from bark, and the beginning to the end of the dying process. Thank you so much for an incredibly informative video! A++ !
I have a question. I started fermenting lichen and now realize I have so much more than I need. Will it keep longer than 6 months if I leave it in the jar? If I want to keep it longer should I strain out the lichen and boil the dye before saving it for later. Thanks for the info in the video so far. My wood pile was covered in the stuff and I got a bit overexcited, which is why I now have way more dye than I need at a time.
Hi Julian. I have had failed DNA extractions. Using the lysis buffer composed 100 mM Tris, 250 mM KCl, and 10 mM disodium EDTA (pH 9.5). The samples in tube PCR to 95°C for 10 min and then mixed with 40 ul BSA 3%. I tested in a electrophoresis the DNA and apparently there is no DNA and in PCR I can´t see amplicons. The fungal tested in my case were Beauveria, Metarhizium, Diplodia and Clonostachys. Considering your experience what is the probably problem ??
Hi, you can't usually see the DNA on a gel prior to PCR due to the low concentration. How do you do your PCR? I have success using 0.5 ul of template in a 10 ul rxn, with GoTaq green master mix, primers ITS1f & LR3 at 500 nM, and a cycle of 95C/3min, 35x(95C/30s, 57C/30s, 72C/90s), 72C/7min. I've seen that too few cycles can result in failure to see PCR products.
@@julianliber4633 Thanks for the quick reply, could you please indicate whether you used PBS? did you sterilize the solution and how; did you use previously sterilized PBS and then dissolve the BSA? Thanks
@@jeancastro1600 because the first solution is buffered, I dissolve the BSA in PCR-grade water then filter sterilize with a low-binding 0.22 um filter.
Beautiful! Is it the Stone-Lichen I used to make a beautiful rich reddish-Brown colour, with chemicals Alun and vinsten (Sw)? For this fine purple: No further chemicals needed for permanence?
I collected lots of lichen on fallen trees on my propety, and it had been soaking in water and ammonia for 2 months now, and no sign of purple at the moment. The liquid is dark brown with some dark red hues, but it yields some uninteresting brownish dirty tone. Do you think it will really turn purple in a couple more months or it should have already started to look like purple is on the way?
@@momentobooks it's also possible that it might not be a lecanoric acid containing lichen. If you have some more of it you can try to scratch off the outer layer to reveal the white cortex, then put a drop of concentrated bleach on that spot. If it turns red, it probably is a lichen that will work to make the dye.
You can cite this paper: peerj.com/articles/12701 Liber JA, Minier DH, Stouffer-Hopkins A, Van Wyk J, Longley R, Bonito G. Maple and hickory leaf litter fungal communities reflect pre-senescent leaf communities. PeerJ. 2022 Jan 27;10:e12701. doi: 10.7717/peerj.12701. PMID: 35127279; PMCID: PMC8801177.
Thank you for this video! I‘ve never seen such a interesting color dyeing idea from lichen. Also it’s interesting that you use to gather lichen chopsticks and a tofu package and cook it with a rice cooker😊
Yes, you could use citric acid or vinegar to lower the pH and get a red to pink color. The color changes if you wash the fiber in a different pH solution.
@@julianliber4633 Is that the same chemically as a plant like a hydrangea bush can grow blue flowers in low PH soil the same plant if put with high PH soil will grow pink flowers?
@@TrudySchwartzBurrill it's similar, in that pH affects color, but the compound is different. Many pigments (natural and synthetic) are pH sensitive, and can be used as indicators. en.m.wikipedia.org/wiki/PH_indicator
The cutlet on the plate's lid is used to help remove bacteria from the fungal isolate. The fungus will start on the cutlet, then jump the gap to the top of the plate where it can usually be isolated without any bacterial contamination.
