Hi How are you? thanks for this video is really so helpful. Could you Please provide in details how to prepare the DNA extraction buffere"Edward", steps by steps
The sheep of Yeshua are all on the same page no matter where in the world they are.(John 10:27) My sheep hear my voice, and I know them, and they follow me: - 예슈아커밍 / Yeshua Coming - John 10:1~30 KJV...... 1Verily, verily, I say unto you, He that entereth not by the door into the sheepfold, but climbeth up some other way, the same is a thief and a robber. 2 But he that entereth in by the door is the shepherd of the sheep. 3 To him the porter openeth; and the sheep hear his voice: and he calleth his own sheep by name, and leadeth them out. 4 And when he putteth forth his own sheep, he goeth before them, and the sheep follow him: for they know his voice. 5 And a stranger will they not follow, but will flee from him: for they know not the voice of strangers. 6 This parable spake Jesus unto them: but they understood not what things they were which he spake unto them. 7 Then said Jesus unto them again, Verily, verily, I say unto you, I am the door of the sheep. 8 All that ever came before me are thieves and robbers: but the sheep did not hear them. 9 I am the door(Yeshua): by me if any man enter in, he shall be saved, and shall go in and out, and find pasture. 10 The thief cometh not, but for to steal, and to kill, and to destroy: I am come that they might have life, and that they might have it more abundantly. 11 I am the good shepherd(Yeshua): the good shepherd giveth his life for the sheep. 12 But he that is an hireling, and not the shepherd, whose own the sheep are not, seeth the wolf coming, and leaveth the sheep, and fleeth: and the wolf catcheth them, and scattereth the sheep. 13 The hireling fleeth, because he is an hireling, and careth not for the sheep. 14 I am the good shepherd, and know my sheep, and am known of mine. 15 As the Father knoweth me, even so know I the Father: and I lay down my life for the sheep. 16 And other sheep I have, which are not of this fold: them also I must bring, and they shall hear my voice; and there shall be one fold, and one shepherd. 17 Therefore doth my Father love me, because I lay down my life, that I might take it again. 18 No man taketh it from me, but I lay it down of myself. I have power to lay it down, and I have power to take it again. This commandment have I received of my Father. 19 There was a division therefore again among the Jews for these sayings. 20 And many of them said, He hath a devil, and is mad; why hear ye him? 21 Others said, These are not the words of him that hath a devil. Can a devil open the eyes of the blind? 22 And it was at Jerusalem the feast of the dedication, and it was winter. 23 And Jesus walked in the temple in Solomon's porch. 24 Then came the Jews round about him, and said unto him, How long dost thou make us to doubt? If thou be the Christ, tell us plainly. 25 Jesus answered them, I told you, and ye believed not: the works that I do in my Father's name, they bear witness of me. 26 But ye believe not, because ye are not of my sheep, as I said unto you. 27 My sheep hear my voice, and I know them, and they follow me: 28 And I give unto them eternal life; and they shall never perish, neither shall any man pluck them out of my hand. 29 My Father(Yahweh), which gave them me(Yeshua), is greater than all; and no man is able to pluck them out of my Father's hand. 30 I and my Father are one. (Yahweh=Holy Spirit=Yeshua) ***************************************************************** The sheep of Yeshua are all on the same page no matter where in the world they are.(John 10:27) My sheep hear my voice, and I know them, and they follow me: The reason people do not know, do not hear, and do not believe is because they are not the sheep of Yeshua.(John 10:26)But ye believe not, because ye are not of my sheep, as I said unto you. The sheep of Yeshua are all on the same page now, no matter where in the world they are, what language they speak, and what color they have. Only Yeshua! Shalom.
Expression of a protein antigen from an mRNA is not what one would consider to be Gene Therapy, its functionality is to achieve an immune response; Gene Therapy is the delivery of a cellular functionality (like an enzyme, or a protein hormone etc) by definition from a gene (DNA) though one could delivery such a functionality using mRNA (message) as well.
I have an SRI GC-8610 in my lab. Unfortunately, nobody in my lab known how to use it. So, I need some help to fix it. I want to use it for CO and H2 detection with TCD. Can you please help me? I just want to understand where carrier gas goes, where sample is injected and steps to start the instrument. Can I send you an email?
What do you need? At the left, you find three diferent holes where you conect the hydrogen and the carrier, the SRI 8610 has an internal compresor that takes air from ambient and uses for the flame if you have a FID
I installed a new bulb while wearing nitrile gloves, and it doesn't appear to be working. After examining it, the bulb has a blue film on it. Your thoughts on what happened?
The answer is that the ANOVA is rigorous for linear (by virtue of linear algebra and you can find the proof in my statistics training manual ... docs.google.com/file/d/0BzoazSMSHd-BNjIxYzA3ZWQtYzNhOC00ZjQyLWE2NTUtNjVmYjUzOGI3NjE4/edit?usp=sharing But pragmatically you can use this for non-linear minimization of squares fits and it is equally useful / powerful because the key is looking at the experimetnal variability, relative to the amount of model structure that is not fit.
@@waynecurtis5886 What amazes me more than the document is the fact that you still kept the comment on your mind 4 years into releasing this vid. And, interestingly, I just showed your video to one of my colleagues few days ago. Anyway, this techniques coupled with linear programming methods helped me a lot in making predictive models. Thanks a lot, your class was one of the few classes that taught me life long skills.
