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Who can I connect with at Stanford? I am almost every week on campus and would love to meet makers and bioprinting reserachers and developers. Appreciate any additional information.
Hello, i have some questions about the same protocol (Fresh + alginate) with cells. Can I use the same temperatures (plate <23*C and needle <10*C ) if I add a cells pellet in alginate solution?
Hello, I am a new user of Allevi Bioprinter. I have previous experience of 3D printer, which was mainly DLP based. And I worked as a support team member(3D printer training, servicing, maintenance and quality control). Recently, I have started work on Allevi Bioprinter for my research study(as a PhD student). It is a great Bioprinter. Now I want to know, Where can I get support for some of my queries? Thank you so much.
hello, I am facing problems in crosslinking of alginate structure and washing/removing gelatin. Please if you could help me in the crosslinking of alginate, concentration or temperature.
Do you have any study material or manual to make a 3d bioprinter for own research? For my project work I have build a 3d bioprinter with a cheap cost. Can u help?
Allevi 3D bioprinters come in a wide range of prices to fit the needs of lab budget's both large and small. Email us at sales@allevi3d.com to receive a custom quote for your lab. You should also definitely check out our protocols page (www.allevi3d.com/protocols/) which has lots of great articles for 3D cell culture. Let us know if you have any additional questions!
Buy an i3 clone, modify the printhead to accept a custom or off-the-shelf syringe pump (probably won't have temp control) and start experimenting with Extrusion rates. Alternatively, build a heating block that can hold a syringe, then modify Marlin to run a vacuum pump as an extruder, substituting vacuum pressure steps for motor steps. It will require a lot of fine-tuning the extrusion rates for materials. Good luck!
It's really up to you! Allevi users work with a wide range of industry-trusted bioinks (such as collagen, matrigel, gelma, and PLGA) as well as their own custom formulations. Most of our users work with a hydrogel mixed with whichever cell line they are working on. Please let us know if you have any additional questions. Thank you!
LAP is the photoinitiator, which when activated by UV light will initiate crosslinking of the methycrylated (gelatin) collagen (GELMA). The company sells GELMA which is why they don't tell you how to make it. It is actually fairly easy to make GELMA if you have lab facilities at hand, a simple reaction, followed by dialysis and then finally lyophilization. Hope this helps.
Thank you for your video. Have you considered adding more information about trouble-shooting z calibration to address needle-breaks and repeated needle+petri plate contact with forces sufficient to break extruder needle?
By the time you get up to speed I would be dead. Every time there is a breakthrough it's always too far in the future. Pity my 3d printer can't do it. So a kidney if it's that Complex why not redesign it to be a simple print. It wouldn't have to be exact but functional to us who are awaiting the organs. Take a car engine complex machine been around for years , then you have electric car engines does the same job but simpler in design. ( get it )
Hi Alex - this is a 28(22) gauge tip. Please let us know if you have any additional questions. We can be reached 24/7 by emailing us at info@allevi3d.com. Thank you!
Hi Francisco! In this video, we are printing with a 27 gauge tapered needle (335 um inner diameter) at room temperature. Let us know if you have any other questions!
Hi Naveen - Yes! You wash away the FRESH gelatin slurry and then can suspend structure in PBS. Please let us know if you have any additional questions. Thanks!
During the first perfusion (with either IPA or warm water) the carbohydrate glass is dissolved and cleared from the channels. Let us know if you have any additional questions!
Hey Andrew, This is a feature of the new Allevi Software slated to be released in June. Please let me know if you have any questions or if you'd like to connect to learn more!
We just published on a similar method using an FDM 3D printer to create molds. I like the use of carbohydrate glass to dissolve away the mold journals.plos.org/plosone/article?id=10.1371/journal.pone.0192752
Hey Peter, super cool. Your y-junction is so clean! And yes, we really like the carbohydrate glass given the inner chip architecture we can obtain and the casting freedom. Thanks for sharing your paper!
