Luminex: Empowering Labs to Obtain Reliable Answers.
Our Mission: To empower labs to obtain reliable, timely, and actionable answers, ultimately advancing health.
Our Vision: We envision a Luminex solution in every lab around the world seeking to obtain timely and confident answers. We aim to lead with transformative solutions that uniquely accelerate reliable answers while reducing the overall cost of advancing health.
Analyzed 294 anti-SARS-CoV-2 antibodies. IGHV3-53 is most common gene (~10%) for targeting receptor-binding domain (RBD) of spike protein. IGHV3-53 antibodies show minimal affinity maturation and high potency,
Which lab completed the sars-cov-# koch process to isolate and reproduce the viral RNA sequence as originally CRISPR designed and isolated per gates patent 10130701
Thanks for reaching out. The fastest way to get help is by filling out the form found on this website www.luminexcorp.com/eu/customer-support/. This should help you get an answer quickly.
Thanks for your comment. We don't have a video of the Stringent Clean Process for the FLEXMAP 3D, but the process depicted in the Luminex 200 video can be performed on the FLEXMAP 3D.
These video's are really helpful as an intro to your technology. More people should have the reflex to go on a search on RU-vid to find stuff like this. And more (all!) companies should invest in building a RU-vid channel to educate (and promote) their products to (prospective) users. Thank you for the vid's! I'm checking the webinars up next ;)
Thanks for your questions, Kevin. Yes, you are correct with your inverse “traditional” qPCR analogy. The MultiCode® bases, isoC and isoG, can only base pair with each other which enables site-specific incorporation of the isobases during amplification. As one isobase is labeled with a fluor and the complementary isobase is labeled with a quencher, amplification is measured by a decrease in fluorescence, rather than an increase (i.e., an inverse “traditional” qPCR). The advantages over TaqMan are in the probe-free chemistry that allows melt to be used to verify specificity. Also, MultiCode requires one less oligo per target, so it’s less expensive and easier to multiplex. When compared to SYBR Green (which also allows melt), the site-specific incorporation of fluor/quencher iso-bases ensures specificity for labeling the amplified targets, whereas SYBR Green is a non-specific intercalating dye that will label any dsDNA (spurious amplicons will also fluoresce with SYBR Green). Hope this helps.
This really looks like a great product! As someone who is working in this space, i love and fear that multiplex assay at the same time. On the one hand its super useful to screen for 20 pathogens at the same time, on the other hand i fear for the sensitivity: What happens when this multiplex test cant detect some pathogens due to lowered sensitivity because of the multiplex design (false negatives)? For qRT-PCR the limits of detection are very low (a few copies of the genome are often enough to amplify and detect), what about the RPP?
Hi Tim, thats'a great question. I think you might find it quicker to speak with a member of our support team. You can reach them 24 hours a day via phone (877) 785-2323 or via email @ support@luminexcorp.com. They're a great team and I'm certain they can help you out!
Performance, Reproducibility and Reliability with a good designed AND optimized qRT-PCR are for sure better than a complex multiplex system. One only needs a good combination of extraction method and qRT-PCR kit, the only downside is that extraction + qRT-PCR takes about 4-5 hours time in total. Would be great to hear some data for limit of detection etc though
Thank you for your comment, Tim. It's true qRT-PCR assays perform well when testing for single pathogens; however, when a lab is looking for upwards of twenty targets per sample, this strategy is inefficient and extremely expensive and it can be difficult to collect an adequate sample from the patient. Per your comments about Performance, Reproducibility and Reliability, the VERIGENE®Respiratory Pathogens Flex Test is quite robust and has a Limit of Detection for viral targets ranging from 4.1 x 10^-2 to 2.7 x 10^2 TCID50/mL. For bacterial targets, the LoD was: 8.1 x 10^2 to 2.4 x 10^3 CFU/mL. Feel free to visit www.luminexcorp.com/respiratory-pathogens-flex-test/for more background on this test as well as other Luminex multiplex assays.
Thank you for your question. The instruments are actually the same and both are manufactured by Luminex. The FLEXMAP 3D brand is owned by Luminex whereas we manufacture the instrument for our partner, One Lambda, Inc. and they rebrand it as the LABScan 3D.
Hi and thank you for your question. Please accept our apologies for the very delayed response. According to the MAGPIX xPO 4.2 IVD Software User Manual, you can “add 70% isopropanol OR 70% ethanol to reservoir RB1 on the off-plate reagent block as indicated on the Cmds & Routines tab.” We hope this helps address your question. Please feel free to contact our Customer Support team with additional questions. 1-877-785-2323 or email support@luminexcorp.com
