But, isn't the shift a binary indicator? It speaks to presence or absence of Target analyte. What about more analog sensing? Like concentration of analyte?
Thanks for the video, I finally understand whats is the "dip". I have a question, It is posibibly to detectc an analyte in a sensor chip if this isn't funcionalited? I mean, when it's only the gold layer
SPR based devices detects local changes at or near the sensor surface by measuring changes in refractive index vs. time. These refractive index changes are plotted in SPR response plots, which are also called sensorgrams. The sensorgrams are used to derive important information about how an analyte interacts with the sensor surface, i.e. binding interaction selectivity and rates. In the case of using a bare gold sensor, if the analyte nears or binds to the gold surface, this would be observed in the sensorgram as an increase in SPR response. By studying the rate of SPR signal response change at different analyte concentrations, quantitative analyte interaction information can be extracted such to what extent an analyte is simply passing by, truly binding, non-specifically binding, or physically absorbing. hope this helps.
Thank you so much!! It really helped me. Then, even if the sensor is pure gold, the sensorgram takes the same form than a functionalized sensor (base line, association, dissociation, regeneration and baseline again) of course it depends for the concentration and the analyte itself. I'm asking because i used to think that a sensorgram produced with a chip non-functionalized looked diferent than a sensorgram with a functionalized sensor.
Worth more than 10 papers on the argument. Is there any chance to have a little comment on how the oscillating electrons feel the surrounding? And by the way if I well understood the method the gold film has to be VERY think right? Either way the electrons on the other face of the gold film couldn't feel the effect of the incident radiaton right?
