Hello this was a great video explaining how MALDI-TOF works! I am currently using the MALDI-TOF on some peptides I'm making and I had a question. The peptides we are making has a +4 net charge but on the maldi we usually see +1 charge mass (m/z) on the spectra and not the +4 charged (m/z) mass. Can you explain this? Thank you!
How does that show up exactly? I mean how do you know that you're seeing +1 charge rather than +4 charge?🤔 I've only analyzed things that had a +1 charge myself I'm afraid🙈😅
The biggest problem with GC-MS is the companies producing them will not compete to drive the price down. We need more research which means lower analytical equipment costs. Everybody who wants to do research can't just happen to get a job with a deep pockets corporation supporting their work. To advance science...lower the tool costs!
I couldn't agree more! However I'm sure some of that cost comes from the fact that these instruments are not exactly cheap to manufacture. But I agree that it would be fantastic if more affordable options would exist!
thank you 🤍😊 I watched it and benefited from you from Palestine 🇵🇸...... I subscribed to you because your explanation is wonderful, simple and beautiful. Thank you again 🤍👑
It seems like they can move between chromosomes as well. You may want to check out this article for more information: 'www.ncbi.nlm.nih.gov/books/NBK557780/#:~:text=Transposition%20can%20take%20place%20from,elements%20independent%20of%20the%20chromosome
Question: what difference is between led ionization and filament tube ionization? ( It can't use the same terminals in a tube noble gas as the terminals of an LED?)
Those two technologies should be combined so you can have a larger area illuminated ( the entire gas tube) with the power consumption of an LED....(efficiency)...the principle of both technologies kinda be the same
Why in your videos the antibodies are connected to the antigen in their heavy chain region? The attachment to the antigen happens on the opposite side of the antibody, in its Fab region composed of both the light chain and heavy chain.
My apologies! This was a mistake on my part when making the video series on antibodies. I plan on making a correction series in the near future and then deleting these.
Great video thanks. One question...if we look at m/z 191 for example, which is triterpanes as far as I remember, we see many peaks from C21 or so up to C37 or so..now, all those peaks from ~C21 to ~C37 are fragments that give off a m/z 191 fragmentation when going through the ionization, am I correct? But the main molecules would have a mass of ~296 (C21) up to 520(C37), also correct?
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Hello, I'd like to ask you a question. So, does the function of the monochromator consist of separating the wavelength that exits the sample after passing through it from the wavelength that is being generated by the flame? Thanks in advance!
Hello. Please how can I explain the rationale behind the buffer gas pressure difference between simulation (0.3 mTorr) and experimental conditions (approximately 5 Torr and 2.8 mTorr) in a linear ion trap
@@biotechlucas4126how does the chart convert to writing because the chart is what I listened in science class living environment and I don’t remember the circle thing if that made sense thanks for replying imma subscribe
@@swishstudios285 Do you mean how you convert from DNA to an amino acid chain? Well you read the chart from LEFT SIDE - UPPER SIDE - RIGHT SIDE, and the only amino acid that all 3 codons will overlap is your amino acid of interest. Let's say your codon is CUA, then it's the SECOND on the LEFT SIDE, the FIRST COLUMN on the UPPER SIDE and the 3rd LETTER on the second row on the right side. The corresponding amino acid is Leucine which is shortened as Leu. In other words, we simply use the chart to find the correct location of what we are looking for but if you happen to see the codon (3 DNA nucleotides) that you are looking for, you can just check the corresponding amino acid (the one next to the codon) immediately and forget about the "proper way" to find what you are looking for. I hope that is of some help!
Depends on the dataset👍 There's a reason why averages are used all the time but when you have data that skews in either direction because of a few extreme numbers, median might be more informative😇👍
Hello, so if I understand, we want to know how many cold antigen are on the solution based on their capacity to replace to hot antigen (which is radioactive), so the radioactivity will decrease if we have a lot and steady if we dont have cold antigene ?
Sorry to hear that. I try my best to explain things both fast and simply. Sometimes I don't get that balance quite right and lean too much towards explaining it fast. I'll try to be clearer in the future!😇
