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🍃 Plant DNA barcoding 🧬 (Lab @ Home) 

Bento Lab
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This week Jenny is attempting to identify a number of plants in her house and garden using DNA barcoding. It's her first attempt at extracting DNA from plants and at running the two plant barcoding PCRs - watch to see how she gets on!
Key to gel pictures:
+ = DNA extractions from fresh leaves
- = DNA extractions from frozen leaves
.1 = 1 in 10 dilutions of DNA extractions in water
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2 окт 2024

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Комментарии : 10   
@NiyomiHouse
@NiyomiHouse Год назад
What is the PCR reaction mix that you used to get the hotShot extractions working? i.e the primer and DNA quantity you used. Thanks!
@lisawerner5480
@lisawerner5480 10 месяцев назад
How fun! Great show! Can you give the recipe and protocol for the proteinase K DNA extractions that seemed to work better? Thanks!
@bashkillszombies
@bashkillszombies 7 месяцев назад
I'd love to know this given that all the kits I've seen are many hundreds of dollars.
@smart9924
@smart9924 7 месяцев назад
Hello! A very informative video thank you for this❤ I do have a question can you please tell me the use of proteinase k in your experiment plus why the ones with rbcl worked and not the one with mat k and why did proteinase k and mat k combo showed bands? Sorry if the questions sound off😓
@fotisvon9943
@fotisvon9943 Год назад
very nice, thank you
@diybiosensors
@diybiosensors Год назад
Such a cool video 🪴🤩
@hasanjahid423
@hasanjahid423 Год назад
can you provide primer details?
@BentoLab
@BentoLab Год назад
Hi! You can find the DNA barcoding primers on our website here: bento.bio/product/dna-barcoding-primer-mixes-200-rxn/
@mijinyawabashir5280
@mijinyawabashir5280 Год назад
Given that there are other molecular techniques available to identify and classify plants, what are the pros and cons of the DNA barcoding technique? Thanks 🙏
@hualon
@hualon Год назад
what other techniques?
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