How to look at Potato starch grains under the microscope

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In this video, I explore the fascinating patterns of potato starch grains under both polarized and dark field microscopy. After preparing a simple specimen, we observe how polarized light reveals the characteristic cross-like patterns of the starch grains, while dark field microscopy provides a different perspective by highlighting the edges. This experiment demonstrates how different lighting techniques can transform the appearance of a sample, showing the unique optical properties of starch.
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Опубликовано:

15 мар 2019

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Комментарии : 50

@chloroplast8611 5 лет назад

your accent makes the video 10x better

@jurajhezel942 5 лет назад

Never would have thought that starch is actually grains... We learn every day :-) Thank you for this. I like your channel :-)

@bigsmoke1195 5 лет назад

Früher in der Schule nie im Biologieunterricht aufgepasst und jetzt ist es das Interessanteste was es gibt ... Ihr Kanal hat mich auch sehr für Mikrobiologie begeistert. Weiter so! :)

@baganatube 5 лет назад

For my entire life I thought starch had a fiber-like structure! Thank you so much for the video!

@Microbehunter 5 лет назад

It is a long polymer of glucose. But it is compact in starch grains.

@ThatTimeTheThingHappened 5 лет назад

I can't help but hear "hello...micro-punter here..." lol I appreciate the videos, thanks!

@chloroplast8611 5 лет назад

his accent is god

@chloroplast8611 5 лет назад

Dork-field

@imaneimane2085 2 года назад

thank you very MUCH 😍💜💜💜💜💜

@akibsmicroscopy1912 5 лет назад

(Again) Is there any good book on microscopy (for amateurs)? Like a book conatining different microscopy techniques, telling about some interesting samples to observe & where to find them, identifying organisms, observing different parts of their life cycle, cultuting them, making permanent & temporary slides, different mounting techniques, about different dyes, sectioning them, dissecting animals, fun experiments with them, different filters (like polarization filter, rheinberg filter etc), illumination techniques (bright field, dark field, reflection etc), characteristics of a good microscope, online microscope communities etc. Or Can You Write A Book On It? (I think it would be very helpful for us).

@Microbehunter 5 лет назад

As a matter of fact, I have already started writing a book on it, but it might still take a bit of time until completed.

@chloroplast8611 5 лет назад

Microbehunter Your accent makes the video 10x better

@krikri3387 Год назад

This is so cool!!! Thanks!

@mukialedori 5 лет назад

very cool! thanks for sharing~

@clshclns8813 3 года назад

Sir Can u please tell me which microscope you have used to view starch granules and what are the magnifications you have used?

@Microbehunter 3 года назад

I ued a conventional bright-field microscope. Don't remember the total magnification, but if was not more than 400x (40x objective). I used also a darkfield filter.

@clshclns8813 3 года назад

@@Microbehunter Thank you very much sir

@JerryOhh 3 года назад

Next investigation Oatmeal and how to look in the microscope and how do work on fats..?

@pinkpanther5379 4 года назад

The potato starch in the polarized photo. 1.) What causes the x shape in the center? (A) Illusion? (B) Internal structure that refracts the light? (C) Something Else?

@mizpahvitales317 2 года назад

in addition, can the digestible starch be differentiated from the resistant starch through this technique?

@KeciapTube 5 лет назад

but can you do chemical reactions under the microscope?? like two liquids that react to one another when they diffuse toghter.

@KeciapTube 5 лет назад

i really hope that Doc sees these comments.

@moot2046 3 года назад

It is possible but not easy to get it right. Even simple setup like microscopy video by dissolving salt with water is not that straightforward

@denise.correatrigoso8212 2 года назад

What type of polarizing filter do you use? I have a compound microscope but it is not polarized 😔

@bendirval3612 5 лет назад

Wait, why did the polarized light make little crosses on each grain?

@moot2046 3 года назад

The starch grain is actually TRANSPARENT (not white) and let the light pass through it and from various angle. In his setup, he is using two polarized filters criss-crossing each other to diffract the light, thus the light pass through the starch grain will also "bend"(diffract) according to the polarizer filter setup. In fact, Light diffraction and colors spectrum through polarizer are complicated topics.

@RifaiAL 5 лет назад

What is the role of the cover glass? Why can't subjects be viewed without cover glass? Also please mention the role of polarizer and analyzer glasses.

@Microbehunter 5 лет назад

The cover glass makes the speciman flat and thin and prevents the water from touching the objective lens. It is also important for increasing the resolution (sharpness) of the image, becasue the optics objectives were designed assuming that there is a cover glass in place. The specimen must be thin so that it is easier to focus (because of the low depth of field of a microscope). It prevents objects from floating vertically. The starch grains are now all flat on one plane. The polarizer only allows light to go through that oscillates in one direction. This light then goes through the starch grains which turn the direction of the polarization. In order to see this, you need a second polarizer. Please watch my video on Vitamin C polarization.

@maklog867 5 лет назад

Have you looked at Pseudomonas fluorescens? P. fluorescens is beautiful under microscope. Pseudomonas fluorescens has ability to produce light.

@rizkifauzi7048 5 лет назад

Up this comment

@roxy_xcxc6869 Год назад

🤔 u got any Microscopic video to compare whats inside an cheap $10/roll 3m HI intensity prismatic reflector vs $99/roll 3m Dimond grade reflector vs $199/roll 3m Dimond grade DG3 cube edition reflector. 🤔. Whats the different!! Microscope video have..!

@PredatorGaming-2007 4 года назад

Which microscope do u use in every video

@mizpahvitales317 2 года назад

may i know what type of microscooe did you use?

@mizpahvitales317 2 года назад

microscope

@Scientist_Albert_Einstein 5 лет назад

Hi, love your videos! Can you make a video on what to do if dust enters the microscope. I purchased one brand new and I constantly take on and off the eye piece to mount a camera for the computer. Now the image i see on the computer has tiny and mid size dark blurry spots. When I take off the eye piece and look inside the microscope, I can see tiny particles on the inside glass.

@Microbehunter 5 лет назад

Hi, thank you for the recommendation. I also have to remove dust from my microscope and will document this when I do this. In the meantime, you might want to watch this one: ru-vid.com/video/%D0%B2%D0%B8%D0%B4%D0%B5%D0%BE-QNSaNyEHcsY.html

@thatoneguyfromouterspace537 5 лет назад

Hi everyone, how can I see living tissues under the microscope? I'd like to watch cells of a living plant divide. Is it possible?

@etooamill9528 5 лет назад

With my knowledge of plant cell I can just tell you that duplication takes aroun an hour. If you are able to keep the cells alive the game is done. I suggest to go to a lake and have the luck to find some monocellulars algae

@ronakhati3147 4 года назад

Hey, have you ever pondered why that cross-pattern appears?

@Microbehunter 4 года назад

Yes. this is an effect from the polarization filters. Can be seen also in other specimens such as Vitamin C crystals. The cross pattern rotates when you rotate the filters.

@roxy_xcxc6869 Год назад

😁 potato starch vs potato flour different microscope video have..!!

@KeciapTube 5 лет назад

incredibile footage. and its just potato.