🔬 How to sterilize equipment such as petri dishes | Amateur Microscopy

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There are different ways of sterilizing labware and culture media. I want to give an overview here.
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23 июл 2024

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Комментарии : 54

@leoavio 3 года назад

Ciao! 2 months ago a knew nothing about microscopes. I was just flirting with the idea to buy one for my kids @ Christmas : looking in internet i found your channel and i got really intererested. You are an excellent divulgator. Finally i bought, tks to your tips, a microscope and i’m enjoying it! The first day of use i found many interesting things to see. Ciao from Italy

@juniericagonzaga9265 2 года назад

Hey Oliver, what if I don't have an autoclave tape and B. stearothermophilus ampule reagent in the lab, how would I conduct QC for the autoclave? All I have is a stock culture of B. stearothermophilus. Thank you.

@EM-ks5my 3 года назад

What is missing here. When you autoclave, you use bag, pounches or aluminum foil to cover what you want to sterilize. Second say, you have a culture in a reusable petri dish, the fist method we used is actual bleaching, basically you pour bleach concentrated on the petri dish in the sink, let it sit and then rinse and repeat. Actually this method is used in food preparation when washing cutting boards for sushi. Because the fish is raw, such cutting board need to be sterile so the fish stays not contaminated. Also when we clean the Bio safety cabinet, or metal surfaces, spray bleach then alcohol. If you are doing cultures, you should invest in an autoclave, a dental autoclave is 500 bucks and something important that biological waste, MUST BE AUTOCLAVED in most US jurisdiction. You can not toss to the garbage bioharzard waste.

@HD-cr8ne 2 года назад

Thanks for this! So the items being sterilized in the autoclave do not need to be wet for the sterilisation to be effective?

@alkeryn1700 10 месяцев назад

not sure your bleaching technique would be useful, as soon as you rince it's not sterile anymore.

@smilefriend7171 Год назад

Very clear explanation, thanks

@williamaitken7533 2 года назад

I really appreciate the advice on sterilization! I am getting into mycology and was hoping to pick up glass petri dishes (previously I had been using plastic, but I don't like the idea of throwing out a bunch of plastic, especially while I'm new and going to make a lot of mistakes). I was already planning on getting a pressure cooker for sterilizing the grain jars, so this works nicely to know that you can pressure sterilize petri dishes. Do you have any advice on storing sterile petri dishes? Especially in a home environment.

@jasonlamboghini 2 года назад

I cover them in foil,works well in a autoclave at 120dg for 30min.

@AussyCC 3 года назад

Good vid thx again microbe hunter

@paulgagie9482 3 года назад

Once again very interesting 🤔

@joanneferndale101 3 года назад

excellent thank you xxx

@Shermack98 3 года назад

Do you have any thoughts on tindallization for sterilizing the medium? I want to grow some bacteria(think blue cheese or yogurt, personally im too young to die) and would like to avoid contamination.

@EM-ks5my 3 года назад

en.m.wikipedia.org/wiki/Tyndallization

@attackemartin 3 года назад

possible "new video" question: how to identify things found in a specimen (for example pond water). Is there a database with images, a good book or something to start with?

@attackemartin 3 года назад

searched three days for a book, but it seems I only had to type in the question here, because I found "Das leben im Wassertropfen" just now. Guess that's the best start

@MicrobehunterMicroscopy 3 года назад

Yes, this book is a very good book. Even if you do not understand German, once you know the name of the organism, you can easily google it to find out more about it.

@HD-cr8ne 2 года назад

Great advice, but when you sterilize in pressure cooker - how do you quickly dry them after?

@MicrobehunterMicroscopy 2 года назад

The heat will cause much of the water to evaporate.

@bakedcreations8985 7 дней назад

Close the petri dish with parafilm and than put it in pressure cooker? Or without parafilm? Without would result in condescation no?

@marcusspitzfaden4653 10 месяцев назад

I have a question: I have some petri dishes which are eventually contaminated by Streptococcus... meaning an blood agar culture on top streps...etc. How can I sterilze it without opening the dishes in a way, that I can - after sterilisation - open the petri dish without danger to get infected ? Sterilization by Autoclave ? Dry heat ? Thank you very much!!!

@MicrobehunterMicroscopy 10 месяцев назад

Autoclave is the standard procedure. sufficiently hot and long (30 min) at 120C about.

@Spartan1-1 3 года назад

I’m only growing bacteria for culturing cilliates so would that be dangerous too?

@MicrobehunterMicroscopy 3 года назад

It's a question of concentration. Keep the number of ciliates high to keep the bacteria low. do this by not overfeeding which causes bacteria to divide quickly. Ensure good oxygen supply.

@Spartan1-1 3 года назад

@@MicrobehunterMicroscopy ok thanks for the advice

@TheQwik512 3 года назад

Instant Pot can be used as an autoclave. As a disclaimer: I would have a separate Instant Pot specifically for only Lab use and not use it for cooking/kitchen use.

@xj11w2c7 Год назад

Just FYI instant pot doesn't get to 15 psi. So if you're using it as an autoclave run things for longer.

@nikonovandrej1985 3 года назад

Hi. Just got a microscope for my son (8 years old). Relatively basic model. Levenhuk 320 Base (no camera, yet..). I want to show something cool to my son, that moves. What you suggest? Pls help a noob! Ofcourse, safe and disposable at a kitchen sink.

@MicrobehunterMicroscopy 3 года назад

I will do a video on this. In the meantime, I suggest that you look at some onion cells. Here is a video: ru-vid.com/video/%D0%B2%D0%B8%D0%B4%D0%B5%D0%BE-quygiQgJ7b8.html Also check out the other videos in the my other channel, where I give suggestions on what to observe. Also this one here: ru-vid.com/video/%D0%B2%D0%B8%D0%B4%D0%B5%D0%BE-8djAmJ_Rwe8.html

@dorsetdumpling5387 3 года назад

Pond water/weed - it’s like a safari in miniature!

@KaladinStormBlessed190 3 года назад

Great video now i have a question, its enough to wash the equipment(tweezers, petri dish, etc) on alcohol to maintain some minimum of safety? I don't think to cultivate bacteria but on the samples we take we don't really ever know what we have there so im a little worried now because i don't have that autoclave thing (i don't even know what its that, now i will search because my first language its no English) and i was wondering if alcohol will be enough to this purpose. Hope to asked it clearly, thanks u so much for this channel, I'm purchasing my first microscope because of ur channel so thanks u a lot and please continue doing videos! I promise to become patron as soon as i can.

@MicrobehunterMicroscopy 3 года назад

Yes, use 70% alcohol. This works better than 97% alcohol. So take 70ml of concentrated alcohol and add 30ml water. This is standard for disinfecting in microbiology labs. Allow for enough time.

@KaladinStormBlessed190 3 года назад

@@MicrobehunterMicroscopy thanks u very much sir!

@Prometheus720 2 года назад

For very small metal equipment, you may consider a bunsen burner. For large metal equipment, you may prefer covering it in at least 70 percent alcohol and then burning the alcohol off with the burner. In my lab this was called flashing and we used it only sometimes.

@KaladinStormBlessed190 2 года назад

@@Prometheus720 thanks u very much 🙏

@diysmallthings 2 года назад

You can use 1% Virkon to clear equipment and surface, more effective than alcohol.

@miguelelento5637 3 года назад

I'm using my glass petri dish for storing dry specimens rather than culturing harmful bacteria.

@JamesJames-ol6kh 2 года назад

An autoclave is not a pressure cooker it's similar to one but with a few key differences

@nishantsen6820 2 года назад

Yes some difference

@pauljmeyer1 3 года назад

Why not clean and sterilize with IPA?

@concernedcitizen8419 5 месяцев назад

Use type one boroscillicate glass petri and other lab ware, completely autoclavable. 🎉.

@diatomsaus 3 года назад

As someone with no formal education in chemistry, my approach is "if I can't read that and there's weird symbols, steer clear" haha.

@potatoesyay7763 3 года назад

Then why does your username involve the word ‘microscopy’??

@diatomsaus 3 года назад

@@potatoesyay7763 Eh, what does that have to do with my illiteracy in chemistry? Microscopy is a massive field and doesn't necessarily involve nasty acids and explosives. I use microscopes, I take photos, I made videos. (I mean, you can see it from my channel, right?)

@potatoesyay7763 3 года назад

@@diatomsaus For gods sake, chill. I just made a small mistake.

@diatomsaus 3 года назад

@@potatoesyay7763 No I'm not on camera, I've never shown my face and don't plan to. I was clearly explaining how microscopy doesn't require expertise in chemistry, as your response to my light-hearted playful comment implied otherwise. If you made a mistake in your comment which I had no way of knowing (and honestly I still don't see the mistake, maybe you thought I typed microscopy instead of chemistry?), the correct response is to concede and preferably admit fault like a mature being, not lash out. No one's demanding anything and no one's attempting to educate you, stop the projection. I know this is social media, but I wouldn't have expected to encounter such a person like yourself on videos that aren't about politics, religion, or competitive gaming.

@potatoesyay7763 3 года назад

@@diatomsaus Yes, sure, ‘lighthearted and playful’. I saw otherwise in the attitude your comment displayed. The “I mean, you can see it from my channel, right?” was an unnecessary add-on, and made your reply very aggressive. Naturally, I lashed back at you. Now, I am sorry if I had offended you in any way, but please say sorry on your part as well.