THESE VIDEOS LITERALLY SAVE MY BIO GRADE LIKE I DONT UNDERSTAND THE WHOLE WEEK IN MY CLASS BUT BAM 8 MINUTES VIDEO AND I KNOW AND UNDERSTAND EVERYTHING THANK YOU
I didn't have an interpreter on the day I did this lab in my class, and I was so lost! I was nervous to find out that this process was going to be on exam! Yikes! However, I finally understand thank you! The closed caption helped me significantly, again thank you SO MUCH! :)
I'm really grateful to have found this...It's going to be my first year teaching AP bio, so your videos are very helpful, and answer many of my questions that I have from reading the manuals...
Hope you guys pursue biology as a major/minor in college, because, in high school, you study the experiments and what happens, as well as what we now understand. But in college, you get to actually perform these experiments and reinforce what you studied in high school and relearned in college!
This video was awesome. I spent a week on this with my IB Bio 2 class and the easy diagrams really helped explain what I was doing in the labs. Thanks, I wish you were my professor!
5 out of 30 students were successful in growing transformed bacteria? Psshhh. No big deal. My class had 100% of the students successfully grow bacteria that glowed. Again, no big deal. We be swaggy like that.
1:20 . For some reason ,for plate 4 , almost 90% of the lawn glow when shined with UV light .. which contract because it is supposed to glow in clusters . So what went wrong ? Anyone know ??
Hello sir.....ph.d in molecular biology involves animal dissections(like where to extract DNA etc from)too as lam from zoology background....please reply
thank you for posting this. I get nervous about labs so I always try to find videos that show me how to do stuff because i get scared of all the stuff i have to do !!
GFP is just a protein, but plasmids are DNA. DNA has to be transcribed into mRNA and then translated into a functional protein. The gene for GFP is useless if there is not a starting point for transcription called a "promoter"--that is the araC that you see in the video. AraC is an inducible promoter, meaning that it is usually off but the presence of some chemical (arabinose) can turn it on. Thus, arabinose will turn on the promoter, which initiates transcription of the GFP gene.
Beneficial is relative to the context of the selective pressures of the environment. A common transformation protocol involves the solution mentioned in the video alongside a heat shock, which will shock the bacteria and "trick" them into uptaking dna. What is not mentioned is that bacteria can get rid of useless plasmids during replication, which is why it is important that these plasmids for lab work have a selectable marker (such as one that encodes for resistance to an antibiotic).
You need to learn how fun it is for your betters to beat up people like you on-line. You go ahead and tell me why a university with 50K student body wants to do a chemical poration when an electro-poration is so much more efficient. u need to tell me!
The reason why divalent cations (basically always Ca++) work is because they can simultaneously be attracted the negatively charged bacterial membranes and the negatively charge DNa. This allows Ca++ to basically bring the DNA closer to the cell. Monovalent cations would only be able to be attracted to one or the other.
