At Home Micropropagation: In Vitro Plants - 2018 Four Seasons Gardening Webinar 

Glad it was helpful!

Glad it was helpful!

Details plz for home based tissue culture

You are welcome!

Robert - glad you liked it!

Glad it was helpful!

extremely helpfull as most of the professional chemicals or additives are not eastly available and are expensive aswell so nice to know I could use household stuff to get growing @@IllinoisExtensionHorticulture

Yes, absolutely. Using a refrigerator is a great option. When I have stored samples at our office. If they are unused media, I put them in the vegetable drawers. They are white plastic and exclude light. For rooted samples, you can keep them in the refrigerator. Make sure the plant is not sensitive to cold temperatures. Since it is a cold environment, the metabolism of the plant will also be slowed.

dompig bryan - Good luck!

It was also known as vitamine B8.

Thank you

No because cinimon has contaminence in the spice itself

The second handout can be obtained by emailing Bruce at brucejb@illinois.edu. There are usually specific questions that follow and Bruce likes to be available to help answer those questions if he can.

Me to plz If there is a chance can you plz forward it to me on Ayman.m.abdelnaby@gmail.com

Hello. Bruce is happy to share the protocol and handout for this presentation. Please email him at brucejb@illinois.edu .

​@@IllinoisExtensionHorticulture ❤

I'm sure Bruce would be happy to share his resources. You may email Bruce at brucejb@illinois.edu

Hello, we do not currently have one on genetic modification that I am aware of. This could be a good topic for the future. I will pass the suggestion on. Thank you!

Yes could be very interesting, like for the marijuana how to put more terpenes ( strong odors, colors etc) Been done in the past months plant smell less (while it was illegal) now that's almost legal all round the world, would be great to recreate the real "skunk piss" smell

I forgot to ask about the hormones. You never mentioned them in the medium preparation

Hello. Bruce is happy to share the protocol and handout for this presentation. Please email him at brucejb@illinois.edu . Bruce will need to do some research into your question about dates and palms. As for your question on hormones here is Bruce's response, in a lab setting, we would make a specific auxin and cytokinin solutions for the different medium. Most of the auxin and cytokinin substances I know of are sold for labs. In the at-home recipe, the inositol, fertilizer and vitamin tablet, provide enough of the needed materials to get the plant to begin to grow and divide. The inositol is used in cell wall biosynthesis as well as the transport of plant hormones. There should be enough plant hormones or the ability to make more hormones in the plant piece we are using. By giving it the proper nutrient rich environment, we are providing it with what it needs to regenerate what is missing.

Never mind I found it.

Would you please tell me where to find it.

@@xaphansinferno163 also curious where you found it please

Which plant u tissue cultured ?

There is a significant difference tissue culture can made a 100 plants where u could made 1 to 10 plants via regular propagation

Yes, a daily multivitamin. I purchased a daily multivitamin from the local pharmacy for the experiment. Any brand will do.

@@IllinoisExtensionHorticulture Do I need to worry about PH due to Vitamin C and Fertilizer?

​@@essoborja763 Great question. The pH of the vitamin and the fertilizer will make the media acidic enough for the nutrients to be available for the explants to take up.

There are lots ofc different gelling agents agar is the most sterile from factory but if the gelling agent is sterile it should work

@@Adamap9 I found out that lab scientists found a better and cheaper alternative to agar, which is “psyllium husk”. They even documented it’s properties to agar and concluded that “psyllium” is better.

@@ShopperPlug thanks I'll update agar to psyllium husk