Bacterial Smear Preparation 

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It's easier to obtain colonies separately from solid media. It's also more likely to carry unnecessary parts from the liquid media.

Water is required only if its a solid culture from a plate but not for broth cultures

​@@Cleome-h4tthnku

Creates a hot convection current sterilizing the air and pushing out any unwanted microbes. Gotta keep the tube sterile at all times too for purity sake

The water will boil out and the bacteria will be destroyed.

Heat damage them so you need to be careful already you can dry it on the flame but with keeping a good distamce in order to prevent it from getting hot

Air drying preserves the morphology of the cell but heating distorts it.

Hi, I'm a microbiology student in my second year. If the sample isn't dry first you are likely to boil the bacteria instantly and all your cells may lyse destroying them.

chase the bag, don't worry about what i'm doing

@@CynicalDuty yes but your contaminating the results so thats wrong. Your also a threat to your health. I have got my youtube money hun x there is a protocol and h&s coshh for a reason if you work for any creditied lab or school and see you doing this, you will get reprimanded.

@@zuluzero4659 bust down, thotiana

@@CynicalDuty Uh, ayo, I been a bad b*tch, cockiana (Cockiana) / And I'm still winnin', Pacquiana (Pacquiana) / Percocets, popiana, killin' everybody beat / Thank you next, Ariana (Ariana)

@@zuluzero4659 I'm not too sure which school you are referring to but at Curtin in Perth, in second-year Microbiology we don't use gloves. It is optional. As for the Lab coat yes it is standard PPE and we all need one. The results are not contaminated by bacteria on our hands nor can the bacteria on the stain infect us as it has been made bacteriostatic from use of the gram staining reagents. The bacteria on the slide are in too significant number to be contaminated by our own natural flora. Note also how they have been heat fixed first so our natural flora would just be washed off the slide if it was present.

Glove could melt and cause a 3rd degree burn

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I guess it's not necessary

you don't need them

Not necessary because culture is created so no contamination chances in sile by hand

youre dealing with fire, glove is not necessary

Could cause a burn if the glove melts on your hand