Тёмный

Bacterial Transformations 

Addgene
Подписаться 22 тыс.
Просмотров 125 тыс.
50% 1

Transformation is the process by which foreign DNA is introduced into a bacterial cell. In this video, we walk you through a standard plasmid transformation protocol and offer some tips and tricks to reduce transformation time and increase efficiency.
For the full written Bacterial Transformations protocol, visit:
www.addgene.org/protocols/bac...
____________________________________________________________________________________________
0:00 Intro
0:47 Starting the Transformation Process
1:41 Performing the Transformation
3:58 Incubation
4:31 Tips

Наука

Опубликовано:

 

4 июл 2024

Поделиться:

Ссылка:

Скачать:

Готовим ссылку...

Добавить в:

Мой плейлист
Посмотреть позже
Комментарии : 15   
@addgene
@addgene Год назад
Addgene does not regularly monitor comments posted here, so we may not see your question immediately. We’d be happy to answer any questions sent to help@addgene.org as soon as possible. Please include the name of the video along with any questions so our support team can help. Thanks!
@Cartoons_90s499
@Cartoons_90s499 Месяц назад
nice video i learn so much
@infinity2394
@infinity2394 Год назад
nice vid 👍
@jeanelmercado1642
@jeanelmercado1642 3 года назад
I have one question Ma'am. When scraping and transferring the E. coli bacteria from the E. coli starter plate to the (+)BLU and the (-)BLU tubes, why is it important that you not scrape up any of the agar in the process?
@addgene
@addgene 3 года назад
In general, it is best practice to scrape agar plates gently because the agar can easily be disturbed while collecting your bacterial cells. The inclusion of agar (or any components that may be present within the agar such as X-gal) in your cellular suspension may adversely affect downstream processes or experiments.
@binusbn5681
@binusbn5681 11 месяцев назад
Is it nutrient agar or mha?
@thefenerbahcesk4156
@thefenerbahcesk4156 5 лет назад
Do you have a video on Asceptic Technique?
@addgene
@addgene 5 лет назад
Not yet, but that sounds like it would be a great topic for a video. Stay tuned!
@omerhayri999
@omerhayri999 4 года назад
SA
@kaysoncanaan5542
@kaysoncanaan5542 2 года назад
you all probably dont care at all but does anyone know a method to get back into an instagram account? I stupidly lost my account password. I would appreciate any tricks you can give me
@tatumblaine9089
@tatumblaine9089 2 года назад
@Kayson Canaan Instablaster :)
@thefenerbahcesk4156
@thefenerbahcesk4156 2 года назад
@@omerhayri999 AS gardaş
@kaustavmitra4210
@kaustavmitra4210 4 года назад
Mam..... how does simultaneous exposure of the cell to ice and hot water bath.... increase the chances of transformation?
@wayne02058
@wayne02058 3 года назад
The precise mechanism is not known. But the theory is they the cell walls open up and become "leaky" allowing transformation to happen
@kaustavmitra4210
@kaustavmitra4210 3 года назад
Thank you mam
Далее
Plate Pouring Protocol
8:50
Просмотров 251 тыс.
Bacterial Transformation
7:02
Просмотров 252 тыс.
Strongest man in the world !! 😱😱
00:16
Просмотров 3,6 млн
Polymerase Chain Reaction (PCR) Protocol
6:21
Просмотров 64 тыс.
Restriction Digest Protocol
5:49
Просмотров 54 тыс.
02 - DNA Isolation from bacterial culture
10:32
Просмотров 58 тыс.
Origin of Replication - Plasmids 101
5:50
Просмотров 67 тыс.
Sanger DNA Sequencing, From Then to Now.
14:37
Просмотров 45 тыс.
Multichannel Pipetting Technique
3:12
Просмотров 35 тыс.
How to Make Chemically Competent E. coli
15:21
Просмотров 49 тыс.
Улучшил свои Apple Watch!
0:25
Просмотров 42 тыс.