BIOLOGY 10 - Basic Microscope Setup and Use 

### Step-by-Step Process for Microscope Setup and Use 1. **Retrieve the Microscope** - Retrieve a microscope from the cabinet. - Carry the microscope with both hands: one hand on the arm and the other underneath the base. - If the microscope is too heavy, seek assistance. 2. **Set Up the Microscope** - Unwind the cord from the back of the microscope and plug it in. - Locate the on/off switch at the back of the microscope. 3. **Prepare the Ocular Lenses** - Adjust the ocular lenses until the number 64 aligns with the white line below the rotating part of the lens. 4. **Adjust the Condenser Lens** - Ensure that the condenser lens is positioned just below the stage. 5. **Select the Objective Lens** - Place the objective lens on low power (4X) and raise the stage to its highest position. - Adjust the light to a medium or medium-high range. 6. **Place the Slide on the Stage** - Open the clip on the stage and slide a microscope slide to the back of the stage, ensuring it is secure under the clips. 7. **Center the Specimen** - Use the knobs on the lower right side to center the specimen over the condenser lens. 8. **Begin Focusing on the Specimen** - Start with the low power objective lens (4X) with the stage in its highest position. - Look through the ocular lens and focus on the specimen. 9. **Increase Magnification** - Once centered, switch to the next highest objective lens (10X) and refocus using the fine-focus knob. - Again center the specimen before moving to the highest power lens (40X). 10. **Troubleshoot Focusing Issues** - If the slide doesn't move, ensure the right knobs are being used and that the slide is properly secured. - If you're struggling to find the specimen, reposition the stage to its highest position and start over at the lowest power. 11. **Observing Unstained Specimens** - Use the stage diaphragm to reduce light if observing unstained specimens. - Try to focus on bubbles or other easily observed objects under the cover slip. 12. **Clean the Lens If Necessary** - If a higher power lens makes the specimen appear blurry, clean the lens with lens paper and lens cleaner. 13. **Post-Use Procedures** - Remove the slide and rotate the lowest power lens into place. - Move the stage to a centered position and slide holder to the left. - Ensure the stage is clean and dry, turn off the light and power, and wrap the cord. - Carry the microscope with both hands back to the cabinet, ensuring the arm faces out and avoiding crowding.

Best video I have ever watched about Microscopes, simple and well explained. Thank you, this have made my life in the Lab so much easier.

00:39 00:49 - Turn ocular lenses until #64 lines up with white line. 00:53 00:56 - Condenser lens must be just below the stage. 01:01 - Put objective lens on low power. Stage must be place at its highest position. 01:11 - Set up the light to medium or medium high range. 01:40 - Center specimen over condenser using the X/Y adjustement knobs. 01:46 01:58 - Make sure compound is centered in field of view on low power. 02:01 - Move to next highest objective lens (10 X) and refocus. 02:07 - Specimen nearly in focus; use fine-focus knob. 02:16 - Center specimen and move to highest power lens (40x). 02:40 - 1. Make sure specimen is centered. 2. Start over at the lowest power and move the stage back to its highest position 02:59 Stamped with love, LINER getliner.com/youtube

Its 9pm on a Sunday, I forgot that I have homework on microscopes due tomorrow so here I am XD

Perfect refresher, doing a microscope test for a job tomorrow and haven’t used one in over 4 years.

Im here bcus my examinations are comin up.Thank you for saving me all my marks if microscopes do come up

Good job of covering the basics. I once was planning to go to Fresno State University, but life took a different twist. California State University was my choice instead. Glad things changed the way they did. Never thought I would endure what I did in Fresno, but God has a way of helping us see the sheer truth.

I’m a English teacher who have a job to teach Science .. and this video helps me a lot

this video is extremely helpful! thanks a lot, I'm applying for a magnet school and we're required to know how to use a microscope of this model and this video helped me a lot! thanks!

we all know that we're here because of a teacher

The iris diaphragm is NOT used to adjust the light intensity, but to improve contrast and resolution. The microscope has a light intensity wheel on the base of the microscope for light intensity control.......

I have science day this Friday. and me and my best friend are doing how to use the microscope for science day. THIS HELPED ME SOO MUCH THANK YOUUU!

good luck, you are curious about everything, you are researching, you have many beautiful features that a person should have

This video was very helpful. It was very thorough and informative. The demonstration was fantastic, well done!!! The girl in this video looks AMAZINGLY GORGEOUS!!! Couldn't take my eyes off of her :)

what type of microscope is it? i am interested in buying one.

Hello sir!May i ask what cell can i use..thnx

thank you so much you saved my practical 😈😈

I did the same thing but for some reasons I keep on struggling in finding the specime :/ what seems to be the problem?

So if I carry the microscope with one hand then I fail?

But how do I use the microscope in the model railroad layout room in the attic?

Such a great video. Thank you for making this video. It helped me alot to do my lab test. Thank you 🙂

Never have I ever seen a Biology class that clean and neat before lol

I got a new microscope, nothing is showing when I view. What do I do?

What model microscope is this? 🔬

Actually helped me in my practical exam

Hai~! First of all, the video is very good and the explanation given is also very easy to understand. However, there is one thing I want to ask (I hope the video maker or friends who see this comment can answer it ^^). So the question is, is there any particular reason that the ocular lenses needs to be set at 64? I thank you for this excellent video and also thank you for being willing to answer my questions :D

Thank you because we will have a experiment about using the microscope!

She touch the objective lenses?

when using light microscope do you start the largest or smallest distance between the lens and specimen /why?

I have a histology final tomorrow, this really helped me . Thx

Thank you so much we ar having a recitation about how to use microscope im dying...

Before using a microscope , you need to wear a laboratory gloves to prevent dirt , dust and fingerprints in the specimen and to the microscope.

Anybody knows name of this microscope please?

it is very interested video to understand working principle of microscope. thanks grate

Wow thank you for good video i didnt know how to plug in my microscope

How many price this Microscopic.

How much is this microscope I have a one but I want to get another one

What an excellent video! I really appreciate the usefulness of microscopes!😊❤👨🏾‍🔬🔬👍🏾If would like one of those.

pls how do u get a dull field of view in ur microscope

Why does number 64 has to line up with the white mark?

1:30 on a beautiful Friday with clear sky and everything and I just realised I have a practical about microscopes at 2 sooo here I am 😂

Hello! There is a problem regarding my microscope... Basically earlier the coarse adjustment was working, and the microscope was going up and down accordingly... But now it's not working ( I think after I took the objective lens away from the slide by using the coarse adjustment, after sometime it got out of control) plz help in resolving the issue, it's a sincere request 🙏🙏🙏

Wow that's great !!! I enjoyed watching it this was very helpful

0:49 It's not lined up perfectly, experiment failed.

Thanks for this only useful video, helping me out

Excellent and highly appreciated...😊💐Thank you

Nice video thanks for making

can't wait to see my kids

How do you center the specimen? Like how do you know when it is centered?

1% people that wants to learn how to use a microscope 99% students that got here because of their science teacher

I have a project on microscope.Is this useful? Can someone who experienced this tell me?

Koehler Illumination and use of the substage aperture to match the N.A. of the condenser to that of the objective?

This video saved me from my performance task ^-^

Thanks ! This really helped me!

Hi, I need to know if a reticle of microscope is similar to stereomicroscope. Because I need to buy one with micrometer.

I’m here because of my teacher and I don’t want to be how are microscopes gonna help me in the future 😀

Man.. Ur all lucky its just assignments For me its a quiz on how to use Microscope I envy u guys

What if you ARE the instructor?

Helped a lot in particle exams

i hope my practical test going well tomorrow

Excellent and highly appreciated 😊thnk you

what is the model name of this microscope

I DONT SEE A SWITCH FOR A LIGHT LR A THING TO PLUG IT IN...WTF DO I DO

Great video !

Is the microscope very expensive?

Deepika . thupalli it gives much information. Thank you......

هل فحص فم اللرحم بالميكروسكوب دلالة على وجود خطر

My teacher is quizzing us how to use a microscope… so… that’s why I’m here

I have to listen to this and write it down because my 1st period teacher wants us to learn to use a microscope

Bsc microbiology final year ...still don't know how to use it

Does anyone know how to use VIS plus ver 3.50 software on computer ? Give me a link or video how to use it . Thanks

Thanks for watching

thanks too much,it helps prepare me for the exam

what is the weight of compound microscope you used.

thanks tomorrow is my practical

EXCELLENT VIDEO💥 THANKS!🌹 FOR YOUR HELP!💥🌹

i'm here because my teacher told me to do so

Great video

Thank you very much 👍 It helped me a lot 👍

We watched this video at school!

🔬 Microscope ka kimat kitna hai Please bataaeia 🖐️✋🤚👋✍️🤳👏🤝

Thank you, the video is short and understandable

Thank you so much it's really helped me

ANG GIMOGGI THIS VIDEO IS VERY GOOD ING~~~

This video is extremely helpful.THANK YOU.

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Thank you, i have learned 😊👍

I taking Anatomy and this is my first assignment

Such a great video...so helpful

Best video 👍🏻👍🏻👍🏻👍🏻👍🏻👍🏻👍🏻

i remember in school when teacher allowed to use microscope :) Very interesting :D

U r really a genius

what i learned is just how to take microscope out and how to put it back in this tutorial

I'm here watching this for my microbiology lab. activity due today. Hope my classmates find this comment, hello! charot.

It was very helpful thks.

It is really fun doing vids isn't it

If we are in std 7 can we use this in what purpose did it use. Nice.

my teacher didn’t tell me how to use this and i can’t see a single thing under it so now i’m watching this hoping i can figure it out before class is over

Interested

very informative, good job:)