BIOSYNTHESIS OF SILVER NANOPARTICLES WITH SORGHUM (SORGHUM BICOLOR VAR TECHNICUM) SEED EXTRACT, CHARACTERIZATION AND BIOLOGICAL ACTIVITIES
Busra Dalgic, Canakkale Onsekiz Mart University, Turkey / Neslihan Demir
Canakkale Onsekiz Mart University, Turkey
(International Conference on Education in Mathematics, Science and Technology (ICEMST)) which took place on March 24-27, 2022, Antalya, Turkey, www.2022.icems... organized by International Society for Technology, Education and Science (ISTES)-www.istes.org)
Nano-sized materials have significant activities compared to their non-nano-sized counterparts. Biological synthesis of nanoparticles, also called green synthesis, is considered the best alternative to physical and chemical methods. Among the various green synthesis methods, plant-mediated synthesis seems to be a suitable method as it leads to the formation of stable nanoparticles in shorter time. Sorghum [Sorghum bicolor (L.) Moench] is a grain belonging to the Poaceae family, contains polyphenols, flavonoids, phenolic acid and other antioxidant compounds. The tannins in its content are also important as anti-inflammatory, cardioprotective, vasodilator and antithrombotic due to their antioxidant properties. S. bicolor var technicum (Körn) Stapf ex Holland used in the study is grown in Turkey and there is not enough literature information about the biological activities of the seeds. In this study, the characterization of synthesized silver nanoparticles from sorghum seeds were clarified by Scanning Electron Microscopy (SEM), Energy Dispersive Spectroscopy (EDS) and UV-Vis Spectroscopy analysis. Biological activities of seed extract and silver nanoparticles investigated with antioxidant activity by 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging method, antimicrobial activity by agar disc diffusion method and DNA cleavage activity by agarose gel electrophoresis method. When we look at the results we obtained within the scope of this study, the IC50 values of the extract and nanoparticles in DPPH free radical scavenging activity were found 102,63±1,27, 60,19±0,94 respectively. An increase in the zone diameter was observed depending on the concentration at disc diffusion method. It showed that the extracts of seeds and nanoparticles cleaved plasmid pBR322 DNA without any external agents. In our research, we observed that sorghum seeds and silver nanoparticles have good biological activity and concluded that the usage areas of both can be expanded. This work was supported by Çanakkale Onsekiz Mart University The Scientific Research Coordination Unit, Project number: FYL-2021-3745.
2 май 2022
