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PCR ko karimulish ne diya. PCR ek polymerase chain reaction hai. PCR ka enzyme Tag DNA polymerase hota hai Jo ki tharmus aquitikus se pratp hota hai. PCR ke three pat hota hai. 1. Denaturation- ds DNA ko ss DNA me Transfer karta hai. 2. Annealing- ssDNA ko RNA primer se connect karta hai. 3. Extension/Elongation- ss DNA per taq DNA polymerase dwara new nucleotide ka synthesis and bistarr hota hai aur new DNA ka formation hota hai. This class is very important for exam 👏
Topic- PCR 1. Full form- Polymarase Chain Reaction... 2. Discovery- Cari Mullus san 1983... 3. Es vidhi me jin ki copy se kuch hi hour me bhut se copy ban jati h.. 4. Jb DNA dono helix ko 100 degree centigrade temperature par garm karte h . Tu h hydrogen bond Tut Jaate Hain. Or DNA ke helix alag ho jate ese Denaturation khte h... 5. Jb DND ko 40-60degree centigrade temperature par cold karte h tu DNA ke dono helix alag ho jate h . Ese Renaturation khte.. 🙏🙏 Thanks you sir ji🙏🙏
मंजिल भी जिद्दी है रास्ते भी जिद्दी है देखते हैं मुकाबला कब तक चलेगा हमारे हौसले भी जिद्दी है..!! Thank you so much sir nice class sir 👍👍🙏🙏🙏🙏👍🌸🌸💮💮🌷🌷🌷🌸🌸💮🌷🌷👍🙏🙏👍
Name upasana (atal 2.0) PCR. (Polymerase chain reaction) PCR के द्वारा किसी DNA खण्ड या जीन की कम समय में लाखों copies बनाई जाती हैं। Discovered by= kary Millie's (1985) PCR Enzyme =Taq .DNA polymaras (thermus akviticus) se प्राप्त PCR Stapes = 3 Denaturation. = double stand vale DNA ko single stand me बदलना Temperature =(94°-96°c) Annealing = single Stand DNA per RNA primer जोड़ते हैं। Temperature= (68°c) Extension = single stand DNA per taq DNA polymaras द्वारा नई nucleotide का संश्लेषण एवं विस्तार होगा और नया DNA बन जायेगा। Nice class sir ji👌👌👌👌👌
Name =bhavana Saini PCR= Polymerase Chain Reaction (PCR) technique, 1985 Kary B. Mullis, allowed to make millions of copies of a scarce sample of DNA. The technique has revolutionized many aspects of current research, including the diagnosis of genetic defects and the detection of the AIDS virus in human cells.
PCR=polimarege chain reaction PCR ki discovery=carry mulis ne ki PCR ka enzyme=Taq DNA (tharmus activicus) PCR ke 3 घटक =denaturation,( 94°--96°c)annealing, extension Thank you so much sir for motivation and Aaj ki class bohot acchi thi ❤❤❤🙏🙏🙏🙏🙏🙏🙏✨✨✨✨ Sir muje book nhi chahiye mere paas already book h mene book buy kr li thi. Aap y book kisi or student ko de dena Thank you so much sir Aaj ki class bohot achi thi ❤❤ 📘📘tannu jangam (old jeet batch ) App me bhi tannu jangam ke name batch le rkha h Thanks vijay education team nd all Teachers 🙏🙏🙏🙏❤❤🎉🎉🎉
PCR= polimarege chain reaction PCR= discovery= carry mulis ne ki PCR ka enzyme=Taq DNA,tharmus activicus PCR ke= denaturation 94*--96*c, annealing, extension Thank you so much sir ji
Name_ Dr Rinki Kumari ( old atal batch) (1)PCR ka pura Name Polymerase Chain Reaction hai (2) PCR ki discover Carimulis ne 1985 me kiya tha (3) PCR ki 3 step hote hai (A) Denaturation 98०c tempretur tak hot karte hai (B)Annealing /primer 55०c_60०c cool karte hai (C) Extension (4) PCR ki Anzyme Teq Polymerase hai (5) PCR ki machine ko Tharmocycler kahate hai (6) PCR ke 1 cycle me 1_2 minut and 20 cycle ke ant tak DNA KA 1Billion copy banta hai Thankyou so much sir today's class is very nice 🎉🎉🎉❤❤👍👍👍🙏🙏🙏🙏
PCR - polymers chain reaction=poly mis ek se adhik and -merase mis banna 2.pCR DNA 🧬 ka multiple copies bnata hai jishe PCR kahate hai 3. Discovery= kary mullis ne 1985 me hui 4. PCR enzyme ke naam jishe tag DNA 🧬 polymers kahate hai 5.ishske three steps hai 1.denaturatin 2. Annealing of primer 3. Extension.=question PCR ke machine ka naam. Tharmocycler hai jisame DNA templet.and nucleotides and DNA polymerase paya jata hai. . name Priti Chauhan atal 2.0 batch thanks you so much sir ❤ ❤ 🙏🙏 and today class is very interesting thanks you so much
PCR ko karimulish ne diya. PCR ek polymerase chain reaction hai. PCR ka enzyme Tag DNA polymerase hota hai Jo ki tharmus aquitikus se pratp hota hai. PCR ke three pat hota hai. 1. Denaturation- ds DNA ko ss DNA me Transfer karta hai. 2. Annealing- ssDNA ko RNA primer se connect karta hai. 3. Extension/Elongation- ss DNA per taq DNA polymerase dwara new nucleotide ka synthesis and bistarr hota hai aur new DNA ka formation hota hai. THANKYOU SO MUCH SIR🙏🙏🙏🙏🙏🙏🙏
Neha gupta Old batch PCR= Discoverer=kari mulis Main enzyme= taq. DNA polymerease PCR =there are three step 1= Denaturation (ds-DNA ko s-DNA me convert karna ) 2= Annealing ( ss-DNA per RNA primer jodna) 3= Extension ( ss-DNA per taq DNA polymerease se new nucleotide se DNA jodha jata hai Thank u sir Sir hum daily comment karte hai per mujhe abhi tak book nahi mila hum batch liye hue hai
PCR) discoved. _______Kary Mullis 1980 me Enzyme._____ Taq DNA polymerase enzyme There are three step ____1... Denaturation. 2.....Primer annealing. 3.... Extension of primers.
PCR __ foll form _ ( polimarese chain reaction) Khojkarta __ karimulish. Y three steps m hoti h 1) denaturation 2) Annealing 3) Extension Isme DNA taq polimarage Enzyme kam m aata h Jo garm gharno m paya jata h Is vidhi ki help se bhut km time m DNA ki bhut sari copies bnay ja skti h DNA fingerprinting m PCR ka use hota h😊 Thanks Vijay education all team ❤❤
1st question= fullform of PCR = polymers chain Reaction. 2nd question= discovery of PCR = calimuris. 3rd question= steps of PCR Denaturation= double 🧬 DNA ko single mai karna . Annealing= ssDNa par rna primaer joint karna . Extension=taq DNA polymerase enzyme ka use kar na and High temperature par DNa ki coping Bana ( Name -Krishna sprot ) atel 2.0 vijay education.❤❤❤❤. Thanks ☺️☺️ very amazing 😍 class dear thanks for you.❤
Name Anu Kumari (Lachey batch 2.o )Bihar PCR (polimarase chain reaction) PCR( karimulis ne Diya tha ) Kam time me lakho me paratiya banata h PCR ka mukhye Enzyme (takDNA polymerase) Tharmas akwitikas bacteria se prapt hota h ❤ PCR ke three type h 1 .Denaturation= ds DNA ko SS DNA me badlna 2. Annealing= SS DNA ko RNA primer se jorna . 3.extension= tak DNA polymerase dwara new necliotide ka synthesis karna . ❤❤ Today's class very helpful guru ji 👍👍👍 vijay h to mumkin h ❤❤
Name- Chandani Singhaniya (Old Atal Batch) 1. Full form of PCR- (Polymerase chain reaction ). 2. Discovery - Kary Mullis. 3. PCR mein Taq-DNA polymerase Enzyme hota hai. 4. PCR mein 4 step hote hai =DATP =DGTP =DTTP =DCTP ❤️Thank you so much Sir ❤️ 💐🙏✨thank you Vijay Sir & all Vijay education team...🙏✨💐
Thankfully session Gurudev 🙏🙏🙏👍👍👍 Today's answer about PCR PCR=polymerase chain reaction Discover by =Kary mulus Three step of PCR=1.Denaturation = ds-DNA strand separate on High temperature 94-96°C..... 2.Annealing =primer attach the DNA strand 3.Extension = Taq polymerase (Tharmus aquaticus )ke through DNA ka replication complete hota hai ...... Thanku so much guru ji 🙏🙏👍👍👍 PCR is a in vitro process and higer copy complete of DNA.......
This lecture is really crucial to watch and it was far different from other previous lectures .. i hope we'll be able to watch these kind of classes in future too because this one was too informative ....we students are really lucky to have a teacher like you❣
PCR का खोज कैरी मुलिस ने किया PCR का full form polymerase chain reaction PCR को थर्मस अक्विटिकस बैक्टीरिया से प्राप्त किया PCR 3 step me pura hota hai (1) Denaturation=इसमे स्टैंड अलग करते है (2) Annealing=इसमे प्राइमर जुड़ते है (3) Extension =इस्मे taq DNA polymerase kam me kete hai PCR se gene कि लाखो में copy bna skte है PCR High temperature me v bna skte hai Thank you so much Vijay team and sir aap lo hmare umeed ho itne kam fees me hum log ko padha rhehai again thank you 😊😊 my name Najni shaheen, old lakshay batch from Bihar...
Name... Chandni Rathour Batch.... Aiims PCR( polymerase chain reaction Discovery ....carry Muiils enzyme ...... Taq DNA polymerase ( उच्च ताप पर DNA Replication करता हैं) * PCR =3 Types of PCR Denaturation.... double stand DNAको single stand मे बदलता हैं(tem.94‘c to 96'c) Anealing... single strand DNA पर RNA primer jodta hai Extension..... single strand DNA per tag any polymerase dwara nai nucleotide ka संश्लेषण एवं विस्तार होगा और नया DNA बन जाएगा Thank you Vijay education today class super🎉🎉
Good evening sir jii🙏 PCR = Polymarase chain reaction... Pcr me km time me lakho dna bnaya jata h... PCR ka khojkarta kary mullis ne (1983) me kiya tha... PCR ke liye enzyme -- Taq DNA Polymeease h... PCR ke 3 step hote h.. 1) Denaturation isme 98°C tk heat krne pr DNA ke dono stand alg ho jate h... 2) Annealing of primer -- isme 60°c tk thanda krte h jisse DNA k stand wapas judne lgte h.. 3) Extension --primer se new DNA stand ta vistar hota h... Thankyou sir jii🙏
PCR(polymer chain reaction) Discovery = Curry mulis. Anzyme= Tek DNA polimrej. Bacteria -- Therms ekvestiks. PCR ke charn= 1.निष्क्रियकरण- इसमे DS DNA के खण्डो का विकृतिकरण होता है। 2. तापानुशीलन -- इसमे SS DNA को RNA उपक्रामक से जोड़ा जाता है 3. प्रसार -- इसमे डीएनए पालीमरेज का संश्लेषण एवम विस्तार होता है तथा नये डीएनए का निर्माण होता है
PCR..polymerase chain reaction.Isme DNA ki copy banate hai tech DNA polymerase enzyme ki help se ( TDP.. hot jharno me Paya jata hai) iske three step hote hai Denaturation DNA strand ko single strand me badlte hai Annealing isme primer ko jodte hai Extension isme nucleotide ko jodte hai . Thanks sir
Rakesh Saini ( vijeta old batch ) Father's name is ramkishor saini Raj. Jaipur chomu PCR( polymerase chain reaction Discovery ....carry Muiils enzyme ...... Taq DNA polymerase (उच्च ताप पर DNA Replication करता हैं) * PCR =3 Types of PCR Denaturation.... double stand DNAको single stand मे बदलता हैं (tem.94'c to 96'c) Anealing... single strand DNA पर RNA primer jodta hai Extension..... single strand DNA per tag any polymerase dwara nai nucleotide ka संश्लेषण एवं विस्तार होगा और नया DNA Thanks you so much sir 🙏 😊 🎉🎉🎉❤❤
Name :- aafrin parveen PCR :- polymerase chain reaction Discover :- kayri mulis Enzyme :- taq DNA polymerase Prapt :- tharmus aquitikus ( becteria) Stap :- 3 1 denaturation:- ds dna ko ss dna m transfer (94- 96) 2 annealing :- ss dna ko rna primer joint (68) 3 elongation :- ss dna ko taq dna polymerase ko new nucleatide Sansleson se dna bnata hai
PCR ( polymerase chain ⛓️ reaction). Discoverer = carymulis. Enzyme of PCR. = Teq. DNA polymerase. (High temperature par DNA replication karvata hai ) Step of PCR = three steps of PCR. 1.Denaturation = DS DNA ko ss DNA me change karta hai ( at 94°-96° C). 2. Annealing = RNA primar add on SS DNA ( at-68°C) ... 3. Elongation = SS DNA par teq. DNA polymerase dvara new nucleotides ka synthesis and vistar hota hai and new DNA ban jata hai name = jyoti saini ( Jeet 3.0 ; Vijay education ). Father s name = vinod Kumar saini. Rajasthan. Thank you so much sir for amazing class ☺️☺️🎉😊😊
Question -:PCR (Polymerase chain reaction) खोज - : कैरी मुलिस (1885 ) एन्जाइम - : DNA Polymerase (Teq polymerase) चरण : -3 1. विकृतिकरण 2. तापानुशीलन 3 . विस्तारण 👉 प्रथम कृत्रिम संश्लेषित प्लाज्मिड हैं। जिसमें क्लोनिंग संवाहक की सभी विशेषता पाई जाती है l 👉 इसका निर्माण Boliver Rodri Quez ने किया Name - Aayushi Bhati Batch - Vijeta 2.0 (Ayushi) F. name - Sumer ji Village - Lawaran Tehsil - Sekhala District - Jodhpur State - Rajasthan Thank you so much sir ji ❤ Aap bahut achaa padhate ho 😊
Name:- Diya juneja Batch:- AIIMS VIJETA (4.0) PCR :- Polymerase Chain Reaction. Discover by:- Carry mulise 3 steps of polymerase chain reaction. Thank you so much vijay sir and vijay team itna acche se padhane ke liye ❤❤❤❤❤
Name -srashti Batch old atal Batch today answer PCR Discover - kary mullis Enzyme - tag DNA polymerase Step -1. denaturation of the template in to single strands 2. Extention of the new DNS strands from the primer Thank you so much sir ji 👍👍👍👍👍👍 bery nice class today 👍👍👍
# PCR ( polymerase chain reaction) * Discovery - Carymulis (1985 ) * Enzyme - Taq dna polymerase * Tharmostable process ( high temperature) Step of PCR .... 1. Denaturation 2. Annealing 3. Extension Use - 1 minute me lakho copies banayi ja sakti hai . Thankyou so very much sir 🙏🙏🙏🙏 Very nice session 👍👍❤❤❤ Priya Verma ( old atal batch )
( PCR) Enzyme -Taq DNA polymerase. discovery -Kary mulis. PCR k steps 1 Denaturation - Dirajjuki DNA 🧬 ko single strand me badlna (temp.94 digry Celsius to 96 ) 2.Amealing - single strand DNA 🧬 pr RNA primar jodte h (68 c) 3. Extension - single strand DNA 🧬 pr Taq DNA polymerase se nucleotide ka sansleson or Naya DNA bn jayega (temp.72 c) Name parwati (AIIMS old vijeta batch) thank you so much sir 😊
Name- sanjana Batch - atal 2.0 PCR( polymerase chain reaction) 1) discovered by carry mullish 2) PCR ka Enzyme Tace DNA polymarage or thermos aquarticus hai. 3) PCR ka steps- three steps 1) Degeneration- the first strep in PCR is degeneration . It requires to separate the Ds DNA sample. 2) Annealing - single stend DNA in joint to RNA. 3) Extension- single stand DNA in join any tag DNA polymarage through nucleotide sanshlashan and produce new DNA. Thank you got all vijay team Today class very nice🎉🎉
PCR (polymerase chain reaction) Jiska uapayog DNA vishaleshan me Kiya jata hai PCR ka khoj - kairi mulis ne 1983 me Kiya DNA polymerase enzyme hote hai jo DNA ke nirman khand nuclear tid ko ekaththada kr DNA aru bnaye hai PCR ke three step hote hai (1)- Denaturation ds DNA ko ss DNA me tranfer karta hai (2)-Annealing ss DNA ko RNA primer se connect krta hai (3)- Extension ss DNA per DNA polymerase dwara nya nuclear tid ka synthesis aur bistar hota hai DNA khand ya gene ka kam time me lakho ka kapiya bnai jati hai Preeti Shah Old atal batch (up) Thanks you so much sir ❤️ ji
Name -Ritu Kumari (Old lakshy batch) PCR (polymerase chain reaction) PCR के द्वारा किसी DNA खण्ड या जीन को कम समय मे लाखो copies बनाई जाती हैं। खोजकर्ता -kary millies (1985) PCR enzyme -tag DNA polymerase Step.1-double stand वाले DNA को single stand मे बदलना Temperature -94°-96°c Annealing -single stand DNA पर RNA primer जोड़ते हैं। Temperature - 68° Extension - single stand DNA par taq polymerase द्वारा नई nucleotide ka संस्लेशन और विस्तार होगा और नया DNA बन जायेगा।
Name -Nisha anuragi PCR - polymares chain reaction Discovery - Karry mullis DNA khand ya gene ki kam samay me lakho me copiyan bnane ki vidhi PCR hai. PCR ka mukhy enzyme - taq DNA polymerase ( thermos acquitikus se prapt ) garam jharano ka bacteria 🦠 hai PCR ke stape Denaturation - double stand DNA ko single stand me badalna ( temp.94°-96°C ) Annealing - single stand DNA (3`sire pr) par RNA primer jorte hai Extension - single stand DNA pr taq DNA polymerase dvara new nucleotides ka sashleshan or vishtar hoga new DNA bn jayega Thank you for Vijay Sir and education team es bar selection ho jaye or kuchh nhi chahiye bs 🥺🙏
Name - Khushboo jaiswal Discovery of pcr - cerry mulish Enzyme of pcr - taq DNA polymerase Stage of pcr - 3 Denaturation , Annealing , Extension Vijay sir or vijay sir ki puri team ko mera pranaam 🙏
Name.. Archana pal Batch.. Jeet 2.0 PCR .. paly marez chain reaction Discovery.. Karry mulish Eske 3 stapes hote h Denaturation .. annealing .. extinction.. PCR ko DNA fingerprinting me bhi use Kiya jata h .. Thankyou so much sir Very nice class sir thankyou thankyou thankyou so much sir
Name sukoon Atal 3.0 batch Question answer no 1. Steps of PCR-1 .Denaturation 2.Annealing 3. Extension. Question answer no 2. Father of PCR= kairi mulis Question answer no 3 Enzyme of PCR= tac . Polymerase ( thermus aquaticus) Thanks a million sir ❤ Very nice class sir 👏👏👏👏 Maja aa gaya padh kar❤❤❤
Name=Shiva singh Batch=atal 2.0 PCR=polymer chain reaction Discovery=carry mulis PCR=DNA ki bahut kam samay me kritiyan taiyar ki ja sakti hain 1 billiun Note= vector ka use nhi Kiya jata hai Thank you sir ❤❤❤❤❤❤❤❤❤❤❤or sir apka dil se dhanyvad vad apko or apki Vijay teem ko 🙏🙏🙏🙏
Name - komal prajapat ( jeet 2. 0) PCR. - polymerase chain reaction Discovery - Carymullis es proccess me DNA/ Gene ki cam time me billions copies bana sakte hai esme high temperature par 🧬 ka replication hota hai esme Taq. DNA polymerase enzyme ka use hota hai
PCR -polymerase chain reaction PCR Discover -keri mulish Three steps of PCR 1.Denaturation 2.anneling 3.Extention Thanku so much sir and vijay education team Batch (jeet 2.0)
1. PCR- polymerase chain reaction k khojkarta (1983) mein setes americea biochemist kerry mules sir ❤ 2. PCR- esme DNA 🧬ki ek bishist gene k lakho ki arbo ki teji se prtiya bnane ki ek taqniq hai. 3. PCR - polymerase chain reaction mein "taq (DNA) polymerase enzymes". 4. PCR - polymerase chain reaction blood k serum k kisi rogjanak ka suchm matra ka pata lgaya ja skta hai, 'Eska upyog aadvic nidaan ( molecular Diagnosis) mein bhi hota hai. Thank you so much sir ji🙏🙏🙏🙏🙏🎉🎉❤❤ SAPNA GAUR - (Old atal batch)
PCR (polymerase chain reaction) discover =Cary Mullis PCR enzyme =teq. DNA polymerase step of PCR three steps of PCR (1) passivation =ds DNA ko single strand me badhlna (94°-96°) (2) annealing =singal strand DNA par RNA perimar par Joshna (68°) (3) expansion = singal strand DNA par teq DNA polymerase dhura nayi nukliyotaid ka visletion ya vistar karna (72°)par
Name Tannubipin bacth lakysha bacth today's answer PcR (polymerase chain reaction) PcR discovered by kary millis Enzyme taq .DNA polymerase Step 1= Denaturation of the template in to single strands Extension of the new DNA strands for the primer Thanks you so much Guruvar very nice session ❤🙏
PcR. Ko kery mulius ne diya tha ( polymares chain reaction ) pcR=maine Enzyme tag DNA polymareas hota hai bactera=thrmus Aquric. Do not requiat =vectar. Key point=thrmostable prossce thank you sar class ak dam nice🙏🙏🙏🙏🫡🫡🫡
Puja kumari vijeta 2.0 batch P.C.R_polimerase chain reaction. Kam time me lakho me dna ki copy --1bilion PCR ke 3 step hota hai 1)---Denaturation---94---96*c.isme dono stand ko alag kiya jata hai high temprature par
PCR ki discovery kerimulis ne ki PCR ki full form polimarase chain reaction h. PCR m taq DNA polymerase hota h jo thrmus eqvitics bacteria se provide hota h jo grm grno m paaya jaata h . PCR ke three step h 1st denaturation 2nd annealing.3rd extention h
Name.... Prashant rajput (Atal 2.0 ) PCR=Polymerase chain reaction. Use - Amplification of gene or DNA fragment without host cell ya vector. Discovery - kery mullish Tharmostable process -High Temp.(90°-94°) PCR Enzyme -Taq DNA polymerase active in High Temp. PCR -3 step. 1.Denaturation-Temp. increase breakdown of Hydrogen Bond-(Double stand DNA Convert to Single stand) Anneling - Primer apply-(DNA Template) Extension - New DNA nucleotide are synthesized through Taq DNA Polymerase.... Thank you so much sir ji 🙏🙏
Name - radha Saini Old jeet batch PCR(Polymarase chain reaction) 1..discoverer =kairimulis 2..main enzyme = tag DNA Polymarase Yh enzyme tharmous aqaticous se pirapt hota hai yh high temperature pr replication karta hai 😊😊 PCR k liye 3 stape hai 1..(denaturation 😊😊temperature =94-96 degree calcious😊😊double stand change single stand) 2..(anealing=primer jodte hai 😊😊temperature =68 degree calcious) 3..(extancing =new DNA formation tag DNA Duara 😊😊temperature =72 degree calcious) RNA primer replication ko start karta hai 😊😊thank you so much sir 🙏🙏🙏🙏🙏
Q= पीसीआर क्या है? उत्तर= पीसीआर का पूरा नाम पॉलीमर्स चैन रिएक्शन विकास= कैरी मूलिस कार्य= किसी डीएनए खण्ड या जीन की कम समय मे लाखों मै कॉपी बनाने की विधि | मुख्य एंजाइम= टेक डीएनए पॉलीमर्स प्राप्त एंजाइम = थर्मस अक्वाटिकस बैक्टेरिया पीसीआर के चरण= 1. Denaturation (94°c - 96°c) 2.Annealing (68°c) 3. Extension (72°c) Thanku so much sir and all' education team ✍️📚❤️📝......
Pcr = polymerase chain reaction Discoverer _ carymulis Enjyme of pcr = Teq Dna Polymerase __high temprature par dna replication karwata hai . Step of pcr __ three step pcr 1-- Denaturation -- Ds dna ko ss dna me change karta hai . __94°c - 96°c par. Annealing ---Rna primar add on ss dna __at 68°c par Elongation --- ss dna par teq . Dna polymerase dwra new nucleotide ka synthesis aur vistar hota hai aur new dna ban jata hai . Dheeraj patel Atal batch 4.0 Vijay hai to mumkin hai 🙏
PCR -polymers chain reaction PCR se anek copy banai ja Sakti PCR ka aavishkar 1983 sets corporation mai America biochemical cari mulis and bio chemist mical Smith ne DNA mai her fer kane ke aavshyak tarike viksit kiye PCR ke upyog=genotype cloning, mutation detected, sequence, forensic, paternity testing Think you sir Sir aap se padh kar esa lagta ki ab to govt college mai ek seat to pakki hai Think you sir🎉🔥💯🥰
नाम मीना बैंच जीत 3.0 PCR =polymerase chain reaction. PCR का मुख्य एंजाइम टेक डीएनए पालिमरेज है यह थर्मसऐसिवटिकस (गर्म झरनों का बैक्टीरिया) किसी डीएनए खण्ड या जीन की कम समय में लाखों में प्रतिया बनाने की विधि के पीसीआर कहते हैं टेक डीएनए पालिमरेज उच्च ताप पर डीएनए रिप्लिकेशन करवाता है 🙏🏻🙏🏻🙏🏻🙏🏻🙏🏻 thank you so much sir ji ☺️☺️
PCR ki discovery Karry mulis na kya tha pcr third step ma complete hota hai yeh one billion banata hai pcr dwara amplification kya jata hai re enzyme DNA ka cut karte hai gele electrophoresis dwara ko DNA segment ka perthaka n size and sadarn booting DNA ka transfer nitrocellulose paper per readioblooting DNA precak ka karya karta hai thanks sir class is very nice Vijay sir and Vijay teem dhanyawad
Name =Shalu Acharya Jeet 3.0 batch( Vijay Education ) ⚡Today's topic - PCR Polymerase chain reaction Discovery - Carry muliis Enzyme = taq DNA Polymerase ( Tharmus Aqvetcs) से प्राप्त ( high temperature per DNA Replication) PCR ..........3steps 1. Deneturation (T = 94° - 96°c) double strand ko single strand mai badlta hai 2. Annealing ( T= 68°c) single strand DNA per RNA primer jodte hai 3. Extension (T = 72°c) Single strand DNA ka taq DNA polymerase se new nucleotide ka संश्लेषण hoga aur new DNA ban jayega # PCR = DNA 🧬 fregment & Gene ki kam samay mai lakho copya bnata hai Thank so Much guruji 🙏& All vijay education team ☺🙏🏻🙏🏻💯
PCR stands for polymerase chain reaction (kary Mullins in 1985).It is a technique of production of multiple copies of recombinant DNA invitro using DNA polymerase enzyme. Working Mechanism Of PCR= This reaction involves three basics steps. 1.Denaturation 2.Annealing. 3.Primer extension. Denaturation=In this Target DNA is heated to a very high temperature (94 degree Celsius). Heating results in the separation of two strands of Target DNA. Each of two strands of Target DNA then acts as template for the synthesis of new DNA strand. This step is called denaturation. ANNEALING=In this two oligonucleotide primers join to each of single standard template DNA. The sequence of primers is complementary to 3' end of template DNA.Annealing is carried out at lower temperature (40-60 degree Celsius). PRIMER EXTENSION - During this step enzyme DNA polymerase synthesize the DNA segment between the two primers. Usually Taq DNA polymerase enzyme isolated from a thermophillic bacteria thurmus acquatics is used in most of the cases. The two primers extends towards each other to copy the DNA segment. This step requires deoxyribonucleoside triphosphate and Mg2,+ and occurs at 72 degree Celsius temperature. These three steps complete the first cycle of PCR and two copies of DNA are formed.For Second cycle these two DNA acts as Target DNA. If these cycles repeated many times (30 cycle) the DNA segment is amplified to approximately billion times.😊 NAME- BALWAN SINGH , HARYANA , CHARKHI DADARI, CHHAPAR.
PCR _ polymer chain reaction . Discoverd by kary mulis Three step 1. DENATURATION 2.ANNEALING 3. EXTENSION Enzyme - tag DNA polymerase PCR high temperature per bhi ho skti ha . Thank you so much sir ATAL batch . Jyoti Gupta
Name- kajal Kumari ( AIIMS vijeta 3.0 batch) State- Bihar Pcr discoverer- carymulas Pcr enzyme - taq DNA polymerase Step of pcr- three steps 1.denateration- double standard dna ko single standard me change karna 2.anneling -- RNA primer ko single standard dna par aad karta hai 3. Elongation- taq polymerase enxyme ss dna ko elongate kar new DNA 🧬 banata hai Thanks for all vijay education team and vijay sir we are providing free mcq classes today❤❤❤❤🎉🎉
PCR = Polymerase chain reaction Discoverer of PCR = Kary Banks Mullis ( Born 28,1944, lenoir, North Carolina, US ) Step 1 = Denaturation of the templete into single strands Step 2 = Annealing of primers to each original strands of new strands synthesis. Step 3 = extension of the new DNA strands from the primers Enzyme required for PCR -= The most used PCR enzyme is ( Taq DNA polymerase ) Taq DNA polymerase is derived form { Thermus Aquatics } an extremely thermophilic eubacterium which grows at temperature above [ 70 ° C ] This enzyme is extremely heat resistant with a half life of 40 min at 95°C
PCR -polimerase chain reaction Dis--kari mulis ne1985 me Isme kam time me dna ke lakho me kopiya banate hai. Pcr -3 step hota hai (1)--Denaturation---94--96*c---isme dono stand Ko alag kiya jata hai (2)----Anniling --68*c---isme rna primer jorte hai (3)--extenstion72*c--per
PCR(polymerase chain reaction) खोजकर्ता= (kary mullis ) 1983 me किया था एंजाइम=(tag DNA polymerase) Point 1= यह अभिक्रिया उच्च ताप पर सम्पन्न होती है Point 2= इस तकनीक में डीएनए replication को बार बार लिया जाता हैं Point 3=one million से भी अधिक डीएनए collins बन जाती हैं Point 4= thermus aquaticus बैक्टीरिया से प्राप्त होता हैं (प्रिया यादव अटल 0.3)
❤Name:-Vinit Kumar Bharti.❤ Lakshya batch 3.0 in Bihar... PCR:- POLYMERASE CHAIN ⛓️💥🔗 REACTION. #PCR का खोजें :- PCR का खोज 1983 में स्टेशन कॉरपोरेशन ने अमेरिकी बायोकेमिस्ट कैरी मुलिस द्वारा किया गया। इसे 1993 में रसायन विज्ञान मैं नोबेल पुरस्कार से सम्मानित किया गया था। #ENZYME:- (Tek) टेक DNA Polymerase enzyme होता है। #Step:- 01) Heat द्वारा विकृतिकारण (Denaturation). 02) Double Stranded DNA को पृथक करना। 03) दो single strand मैं विभाजित किया जाता है। 04) जो 90°C🌡️सेल्सियस से अधिक तापमान पर होता है। ❤🚘👑Thankyou So Much sir 🎉🎉🎉❤...?
Name:- usha Pandey Batch:- old atal batch Topic:- PCR( polymerase chain reaction) 1:- discovery of PCR by carymullis 2:- the main enzyme of PCR is taq DNA polymerase 3:- three steps of PCR 👉 denaturation:- ds DNA change into ss DNA at 94 to 96 degree c 👉 Annealing:- RNA polymers add on ss DNA at 68°c 👉 elongation:- at this last process use taq DNA polymerase for synthesis a new nucleotide which bind to ss DNA then form a new DNA Thank you so much sir
PCR is stand for - polymares chain reaction and PCR me three steps hai Denaturation, anniling,.extension And PCR ka use - DNA fingerprinting, pathogens diagnosis etc.
Very nice class 😅😅 Name khushwant daiya Old vijeta Bach Rajsthan 1PCR discoveri कैरी मूलिस in 1985 2एंजाइम टेक dna poly merej इसे थर्मस एक्वेटिस से प्राप्त किया गया है 3PCR ke चरण Denaturation , annealing , extension Thanks for nice class sir ji
Name=sakshi Gautam Batch=atal batch PCR=rolymer by karymuils Three step. (1)denaturation (2)annealing (3)extenston Enzyme=tag DNA polymerase PCR high temperature perbhine skti hai Nice class sir 🙏🙏🙏🙏🙏🙏🙏🙏🙏🙏👍👍👍👍
Name - Tamanna Suman Batch - jeet 3.0 batch State - Baran , rajasthan Topic - PCR 🌟 PCR ( polymerase chain reaction ) 🌟 Discovery - केरी मुलिस ने 1985 मे 🌟 Enzyme - DNA Polymerase ( Teq polymerase ) 🌟 PCR staps - Three staps 1 Denaturation 2 Annealing 3 Extension 🌟 इसे Thermas Aquaticus जीवाणु द्वारा प्राप्त किया जाता हैं। 🌟 इस प्रकिया मैं उप्क्र्म्को के दो स्मुच्यो व DNA polymerase Enzyme का उपयोग करते हुए Envitro विधि द्वारा जीन कई प्रतिकर्तिया बनाई जाती हैं। ❤Thank you vijay sir and vijay teem 🙏👍hma itna accha padna ka liya✍️ or motivated karna ka liya 👍👍👍
PCR _Polymerase chain Reaction Pcr ka khog _कैरी मुलिस ने किए Pcr का मुख enzyme _Taq DNA Polymerase प्राप्ति _ थर्मसऎक्विटिकस Pcr three steap (1) Denaturation (2) Annealing (3) Extension
PCR- polymerase Chain Reaction. Poly means -ek se adhik. Merase means -formation. Means -ek se adhik copy ka banna. Discovery by - kary mullis in 1985 step _ There are three steps. 1-Denaturation 2- Annealing 3- Extension of primer. One single DNA ------------Many DNA copy. The name of PCR machine is Tharmosaicler. My name - Nitendra Raj (old atal batch) from Jhansi
PCR=POLYMERASE CHAIN REACTION DISCOVERY=KERY MULISE 1983 ENZYME=TEQ DNA POLYMERASE PCR STEPS= 1 DENATURATION 2 ANNILING 3 EXTENSION Santosh (old jeet beach) THANK YOU SO MUCH SIR JI ❤
Today topic ✍️ PCR Full name पॉलीमरेज चेन रिएक्शन P C R step (3) *1.विकृतीकरण *2. तापानूशीलन (annealing) *3. विस्तारण पीसीआर किसी दिए गए डीएनए की कई प्रतिया बनाने के लिए एक मानक आणविक जीव विज्ञान तकनीक है = एंजाइम टेक डीएनए पॉलीमरेज = खोज केरिमूलिस नाम जसी बेस विजेता 4.0 Thank you so much sir and all vije education fimily ❤✍️🌍 Today class very interested
PCR (polymerase chain reaction) Discoverer carymulis Enzyme tac DNA polymerase Steps 1. Denaturation of the template in to single strands. Step 2. Annealing.the reaction is cooled to enable the primers to attach to a specific location on the single stranded template DNA by way of hydrogen bonding.
Vijeta bach Shivangi Gupta Father -केरी मूलिस Time kam , Billins me copy, tac DNA polymerase, 3 istep me hota hai, Vector ka proyog nahi hota hai Sir mai dely class dekhati hu leki kabhi bhi aap mera nam lete hi nahi ho Thank you sir
Name.Durgesh Barya Betch vijeta old betch District Lalitpur u.p. PCR.polymarage chain reaction Iske 3 step hote h 1- Denaturation. Isme ds DNA ko ss DNA me transfer krta h 2- Annealing - ss DNA ko RNA primer se connect krta h 3- Extension/Elongation - Ss DNA per Teq DNA polymerase duwara new nucleotides Ka synthesis and formation hota h fir new DNA ka formation ho jata h 1.PCR की खोज सर केरी मुलिस ने1985 me की थी 2.Enzyme teq DNA polymerase jo tharmus aquiticas bacteriya se prapt kiya jata h 1 step me tem. 94 degree 2 step me 40 - 60 degree 3 step me 72 degree hota h 3.pcr se bhut km time me milian me DNA 🧬 ki copies ki ja skti h Thank you so much sir ji very nice class and amazing class 😍🤩🙏🙏🙏🙏🙏🙏🙏
PCR polymerase chain reaction PCR discovery karimulish ne ki 1983 me Enzyme Tag DNA Polymerase Jo ki thermos aquatic bacteria se prapt hota hai Jo high temperature per DNA ka replication karvata hai PCR ka three step hote h 1 Denaturation - doblule stand DNA ko single strand DNA main badalna (T=94•C- 96•C) 2 Annealing - ssDNA ko RNA primer se jodna (.T= 68•C 3 Extension single strand DNA per tag DNA polymerase par nucleotide ka sanshleshan hoga naya DNA ban jaega (T=72•C)
2,Anealing of primer,esme 20se30, nucleotides से मिलकर बना है,3 एक्सटेंशन ऑफ़ प्राइमर बचे हुए खण्ड पर यदि कंप्लिमेट्री nucleotiedo के छ आर को जोड़ दिया जाए तो एक कंप्लीट dna बना लेते हैं 4 प्रकार के nucleo. की आवस्कता है A G nitrojan क्षार C pentose serkara Tphosfet ग्रुप,nam Munni pal up old ATL Bach 🙏🏻
PCR - Polymerase Chain Reaction. Discoverer - Kary Mullis , in 1985 Enzymes - DNA Polymerase ( Taq Polymerase ) Primers Steps in PCR - 1. Denaturation - The DNA I'd heated at 94°C & Denatured by breakage of hydrogen bonds between complementary base pairs. 2. Annealing - The rxn mixture is now cooled at at a temperature of 40 - 65 °C for a minute. It is optimum for binding of Primers pairs with DNA template. 3. Extension - It is done at 72°C where Taq Polymerase synthesis the DNA region btw primers using DNTPs (Deoxynucleoside triphosphate) or deoxyribonucleotides and Mg2+.so , DNA segment is copied. After a numbers of cycle, 2^n number of molecules are generated. ★One billion copies are made in approx 30 cycles of PCR. Applications - ★ DNA fingerprinting ★ Diagnosis of plant pathogens ★ Gene Therapy ★ Prenatal Diagnosis ★ Diagnosis of specific Mutations, etc. Name - Arshad Ali Old Jeet batch Add - tahsil Khajuwala Dist. Bikaner Rajasthan I think this name is new for u sir but I am watching u from 2023 . Thank you sir 🙏🙏
Name Antima nagar Batch old Jeet batch P.c.R=polymerase chain reaction... Discover by=kerimulish ne 1983 me ki P.c.R tharmas acvaticas se prat enzyme h Eske 3 step hote h. 1=denaturation 2=Annealing 3=extension..... P.c.r ka kary lakho me copy bana h
Aaj ki sabse achhi class thi very very thanks guru ji ❤❤ The polymerase chain reaction (PCR) is a method widely used to make millions to billions of copies of a specific DNA sample rapidly, allowing scientists to amplify a very small sample of DNA (or a part of it) sufficiently to enable detailed study. PCR was invented in 1983 by American biochemist Kary Mullis at Cetus Corporation. Mullis and biochemist Michael Smith, who had developed other essential ways of manipulating DNA, were jointly awarded the Nobel Prize in Chemistry in 1993 PCR step (1) denaturation of the template into single strands. (2) annealing of primers to each original strand for new strand synthesis. (3) extension of the new DNA strands from the primers.
