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Really interesting I've had about 15 blood tests in the past month and a half to find out why my liver is messed up and always wondered what happens when my blood is sent away
1.) Why wash the reagent RBCs? Shouldn't they come from the manufacturer already "clean"? 2.) Why add the AHG(Combs reagent) to the Screen cell tubes? I thought AHG detects auto antibodies attached to the PATIENT RBCs, no? What am I not understanding?
1) I don't believe I said anything about washing reagent cells. That is not something we usually do.2) I think you need to go back and re-read what the function of AHG is and its role in the indirect antiglobulin test.
Patrick Tracy My apologies, I re-read a bunch... So...At the IS phase we are looking to see if IgM have attached to the reagent RBCs because IgM are large enough to cause visible agglutination. We do the 37°C and AHG stage because we're trying to see if IgG have attached (sensitized) to the reagent RBCs. IgG are warm-reacting so they will be more likely to attach to RBCs at the higher (37°C) temp .. especially with the help of LISS. However, even though the IgG are attached, aren't they still too small for visual agglutination? and that's why AHG comes in. AHG will attach to IgG (both bound & unbound?) but we want to make sure it only attaches to the antigen-bound IgG so that's why we wash the solution (PT serum & reagent cells) - to remove any unbound IgG or other proteins that might get in the way of AHG binding to the antigen-bound IgG. Is my understanding correct?
Patrick Tracy Thank you for your reply. May I ask why it is necessary to observe for agglutination after the incubation but before the addition of AHG? If agglutination was found after incubation would you still continue with AHG?
Some blood banks will not spin and read the tubes after the 37C incubation. Yes, even if you have agglutination at 37C you still add the AHG, spin and read.
Hello Aysha Screen cells are used to check patient plasma/serum for clinically significant antibodies. If the patient will receive donor RBCs, we want to first make sure the patient doesn't have any preformed antibodies to the donor RBCs. The first step in that process is by running an antibody screen. If any vial at any stage of the antibody screen is positive, then an antibody identification panel is performed. Screen cells usually come in two or three vials and are pooled from blood donors. They are designed to have most clinically antigens present on their surface.
It depends on the laboratory's standard operating procedure (SOP). It is possible to do what is called an immediate-spin crossmatch, which means you test the patient plasma and donor RBCs at immediate spin and if they are compatible, the unit is issued to the patient. Every blood bank has to determine its own SOPs.
So if i did this test i dont need to do the cross match test right? What i dont get is how i am going to give a blood to the patient ?? In the cross match we used to analyize the blood bottle but now where are not using a sample from the blood bottle so how is this gonna happen ?? And forthermore if the result had agglutination after the check point it means the patient is compatible ?
Medical Laboratory Scientist, which is a 4-year degree. These videos were made for my Medical Laboratory Technician students, which is a 2-year degree.
1. If a patient is Rh+ and SCI+ or SCII+, does that mean the patient has anti-D antibodies? 2. If a patient is Rh= and SCI+ or SCII+, does that mean that this patient also has anti-D antibodies? 3. Or do SCs have nothing to do with Rh? Or at least not specific to Rh and is for ABO or both (Rh/ABO - it just says there’s an antibody)? Thanx.
What do Rh+ and Rh= mean? Yes, we associate Rh with D with performing the ABO/Rh typing, but there are around 50 antigens in the Rh system. In the antibody screen, typically we find D, C, c, E, e. I can't answer your question.
@@patricktracy9947 apparently this is the case of you know everything and I know every little cuz I can’t even ask a proper question. Lol. My bad. How about this…what is the purpose of screen cells?
@@kazuy0shi558 The purpose of the antibody screen is to check if a patient has any clinically significant warm IgG antibodies to antigens that are commonly involved in transfusion reactions.
@@patricktracy9947 oh ok. Thank you! ❤️ what’s the difference between SCI and SCII? If they’re pos does that mean we have to run a panel? If so, just Rh, Kell, Duffy, and Kidd? Or Lewis, Lutheran, and all the other cold ones? Sorry for all the questions.
@@kazuy0shi558 The antibody test typically has two or three cells lines (SCI, SCII and SCIII). Three is better than two because it will be better at detecting clinically significant antibodies. Yes, if any part of the screen is positive, you must do an antibody identification panel which will include all the antigens you have listed and more.
In an antibody screen, we are looking for antibodies in the patient's plasma. In order to detect if there are any autoantibodies or plasma-related issues that may cause a false positive in testing, we include an auto control. The auto control is two drops of patient plasma and one drop of a 3-5% suspension of patient RBCs.
Again, this is a complicated question. I don't feel it is my area of expertise. My education, training and experience are related to medical laboratory science in a laboratory. I am sorry, but I don't feel comfortable talking about matters related to a person's health.
Hey one thing i dont get about this is, how do you know if a person is negative or postive ? Or r1, r2, R, Kell etc?? Plz explain briefly. I know how to do the test but i cant do the conclusion if the person is pos or neg blood group
+Shanelle Pou Hi Shanelle,Your question is too big for me to answer here since there are many causes of ABO discrepancies. I am assume you are in a blood bank course or have been, so you must have a textbook. Does your book discuss this topic? You can always look on the Internet for sources.Here is a brief explanation from an MLT program about solving discrepancies.www.austincc.edu/mlt/clin2/abo1.html
Hi Saskia, although most blood bank testing has gone to automation, tube testing is still used. Also, when an automated system goes down or stops working, tube testing is what is used to continue.
I teach my students that when reading tube reactions, they must assess agglutination AND the background. 2+ is several smaller clumps and a clear to cloudy background. I wash the cells with 0.85% isotonic saline.
+fah moh The procedure for the antibody identification panel is the same as the antibody screen. The only difference is that there are more cells. A screen will have 2-3 cells, whereas a panel will have 8-16. See my video Blood Bank - Antigram Reading Updated for identifying antibodies.
To prepare, I would watch my videos Reagent Rack, Record Sheet, 3% Suspension, Tube Reaction Grading, ABORh and Antibody Screen. These should give you enough basic information to get you started in your blood bank clinicals. Your clinicals are about applying what theoretical information you have learned. The videos focus on that process, therefore they will help you get started. One thing to keep in mind, the videos show one method or explanation, but your clinical facility may do things a little differently, so keep an open mind.
So if i did this test i dont need to do the cross match test right? What i dont get is how i am going to give a blood to the patient ?? In the cross match we used to analyize the blood bottle but now where are not using a sample from the blood bottle so how is this gonna happen ?? And forthermore if the result had agglutination after the check point it means the patient is compatible ?
@@patricktracy9947 when a paitents needs blood transfusion we normally do the cross match test in order to give the compatible blood bottle right Now in the screening test i got confeused how we will give a blood bottle ? Did u get my question right
@@selena5979 Every blood bank will have its own SOPs (Standard Operating Procedures) which will determine which testing is performed before blood products can be issued to a patient. All patients who may need blood products will have a type and screen done on their blood. I would say most, if not all, will do a crossmatch. There are various tpyes of crossmatches including computer and serological. Serological can be immediate spin or AHG. All testing depends on the lab's SOPs.
