Hydrophilic interaction liquid chromatography, or HILIC, is a powerful mode of liquid chromatography (LC) that uses polar stationary phases and high-organic initial mobile phase conditions for the separation of polar analytes. Buffers are very important for HILIC because the ionization state of both the analytes and stationary phases will impact the separation. Here we walk through a routine to optimize your buffer concentrations.
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Additional Resources
How to Avoid Common Problems with HILIC Methods | www.restek.com/articles/how-t...
Simplify the Switch to HILIC with Raptor HILIC-Si Columns | www.restek.com/articles/rapto...
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Restek Recommended Tools & Parts
Raptor HILIC-Si Columns | www.restek.com/search/analyti...
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28 окт 2019
