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Care and Maintenance of a Liquid Nitrogen Tank - Mizzou Repro

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A liquid nitrogen tank is a cryogenic storage container that can be used for preserving and storing semen for an extended period of time. Semen is a significant cost in an artificial insemination program, and maintaining a liquid nitrogen tank’s integrity is key to protecting that investment. Carson Andersen shares how to maintain and monitor a liquid nitrogen tank.
For more information, see University of Missouri Extension publication G2002: Care and Maintenance of a Liquid Nitrogen Tank, available at extension.miss....
Video by Dr. Jordan Thomas, Beef Reproduction Extension Specialist from the University of Missouri's Division of Animal Sciences in the College of Agriculture, Food and Natural Resources.

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16 авг 2024

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Комментарии : 20

@mrjay6483 Год назад

Should you build a special room in the barn to store the Semen Tank ?

@MizzouRepro Год назад

In most cases that is probably not necessary, but it does need to be stored in a location that minimizes the risk of it getting knocked over, dented, etc.

@benson621 2 года назад

should the room storing LN2 have ceiling exhaust or low level exhaust one says ceiling exhaust as nitrogen is lighter than oxygen to so extract effectively at high level; another says the when accidentally pouring some LN2 on the floor, low level exhaust can exhaust out nitrogen which is just been vapourized, not letting them even have chance to reach mid/high level of the room

@MizzouRepro 2 года назад

Good questions. For most small-scale situations storing/handling tanks on the farm or ranch (e.g. in a room with one or two tanks), the main thing is that there is ventilation into the area in general. If no new air is coming in, high levels of N that build up in the room could be dangerous. For larger-scale industrial settings that pose more of a potential for that sort of thing even with ventilation, we suggest consulting an engineer about ventilation design. It will take some situation-specific math to ensure both an adequate new supply of oxygen as well as efficient removal of nitrogen.

@damasoniyaramesh2612 7 месяцев назад

How many days store in crayo cans liquid nitrogen

@MizzouRepro 7 месяцев назад

Cryopreserved material can be stored for years in liquid nitrogen tanks. Just be sure that the tank remains charged with an adequate amount of nitrogen.

@THORFANG Год назад

How long would it take a 6L container to leak liquid nitrogen until completely empty, if the tank was never opened once filled, since all liquid nitrogen containers leak at least some gas

@MizzouRepro Год назад

Unfortunately, there is no good way to answer that. It will depend on the shape of the tank to some degree as well as the effectiveness of the design for minimizing heat transfer across it. Most tanks will have a spec sheet indicating the expected length of time they should maintain liquid nitrogen.

@davidgraham1209 Год назад

I have a 3 L tank in the storage room. I planned on using has a gas water heater with a pilot light. Is this a concern?

@MizzouRepro Год назад

Nitrogen gas is non-flammable, so no issue from that standpoint. As always though, make sure the room is somewhat ventilated if occupied.

@AsimKhan-wp2lk 2 года назад

Miss Mizzou Repro, if the neck is leaked, how it can be repaired?? Regards

@MizzouRepro 2 года назад

Probably best to consult with the tank manufacturer about the repair options for that specific model tank. But in our experience, a damaged tank often simply needs to be replaced.

@refiloeranthamane4938 2 года назад

Hi.may i ask please. What does it mean when I see liquid inside the tank down there. But there is still gas coming out

@MizzouRepro 2 года назад

That is normal. You want there to be liquid in the tank. Nitrogen has a very low boiling and will constantly be evaporating ("boiling off") out of the tank, which is why gas will be observed as well. Just monitor the level of liquid nitrogen still remaining down in the tank and make sure that it never runs completely out of liquid nitrogen.

@bennettsaw2999 Год назад

Hi, may i know when should I refill my nitrogen tank? Should the nitrogen level higher than the straw the whole time to preserve its quality?

@MizzouRepro Год назад

Just be sure the tank always contains some level of liquid nitrogen. The liquid nitrogen does not need to cover the top of the canisters/canes, as the nitrogen gas inside the tank is also sufficiently cool-so long as there is still liquid nitrogen in the tank.

@maliabornitz9235 Год назад

Can I leave the specimen pails out if only using nitrogen by pouring out for procedures.

@MizzouRepro Год назад

Malia, could you clarify what you mean by your question? It is important the cryopreserved gametes or embryos remain covered in liquid nitrogen for storage, so if intending to us nitrogen for other purposes by pouring it out, I would suggest using an empty tank for that.

@maliabornitz9235 Год назад

@@MizzouRepro Yes so we have a tank that came with the specimen pails but we do not need them. Can we leave them out and just use the plug? Will it seal properly.

@MizzouRepro Год назад

@@maliabornitz9235 I think I understand the question now. Yes, you could remove the canisters from the empty tank that you will just be using to store liquid nitrogen. The rate of nitrogen evaporation out of the tank should not be appreciably different.