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Cell proliferation and cytotoxicity assay 

CellGS
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A quick video explaining Orangu, a non-cytotoxic, sensitive colorimetric assay for the determination of cell viability.
Learn more here: www.cellgs.com/items/cell-cou...

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7 авг 2024

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Комментарии : 25   
@fran16justo
@fran16justo 2 года назад
Really nice!!
@45bounty
@45bounty 3 года назад
thk's so much..very helpful -)
@user-tf3kr6cl2f
@user-tf3kr6cl2f 2 месяца назад
Thanks so much.. very helpful ❤ Julia
@FA-oz4cd
@FA-oz4cd Год назад
Hi Julia, can you explain how do you generate your calibration curve?
@Ibrahim920
@Ibrahim920 2 года назад
Thanks Jolia Plz tell me how you determine LD50 plz
@zunikaamit
@zunikaamit 3 года назад
Hi Julia, your video shows how to use the product with suspension cells. How about the adherent cells?
@michaeljones2781
@michaeljones2781 3 года назад
Hi Zunika, Orangu works great with adherent cells.
@scholarshipnetworkguideoff7988
Great work done, I am PhD Scholar in China, Wish to work with you
@teferaworku8842
@teferaworku8842 4 года назад
Hi Julia I want to use the protocol that you have been demonstrated last time using column chromatography would you please! share me the the type of chemicals(reagent), column chromatography. And also would you send me the protocol and the required chemicals and instrument for the method. Thank you Julia
@CellGS
@CellGS 4 года назад
Hi Tefera, The Exosome Isolation range protocols can be found here: www.cellgs.com/items/exosomes/exospin.html. Please do not hesitate to email us at tech@cellgs.com if you have any further questions. Thank you!
@biotical7697
@biotical7697 3 года назад
Hi julia I Tried orangu assay for HCC cell line(Hep3B) , i seeded almost 7500 cells in per well(96 well) and it incubated , but it doesn't work i tried many times but i didn't get result do you have any suggestion?
@michaeljones2781
@michaeljones2781 3 года назад
Hi I'msadaf, please contact tech@cellgs.com for support
@rjkim5000
@rjkim5000 3 года назад
Nothing on quantified data analysis and results interpretation?
@michaeljones2781
@michaeljones2781 3 года назад
Please have a look here. www.cellgs.com/exosomes.htm where we show examples of data generated using exosome purified using Exo-spin.
@luisp.9710
@luisp.9710 2 года назад
Hi. Can this kit be used after 6 months (shelf life) and get reliable results?
@michaeljones2781
@michaeljones2781 2 года назад
Hi Luis, Yes, the kit will still be reliable after 6 month's storage
@dr.senthilkumarkalyanasund6099
@dr.senthilkumarkalyanasund6099 2 года назад
Concentration in 100microlitres of Sample is?.. Reply...please... thanks
@CellGS
@CellGS 2 года назад
Hi Senthilkumar, you can read the protocol here www.cellgs.com/products/oranguand8482--cell-counting-solution.html
@gogomahmoud6807
@gogomahmoud6807 Год назад
From the videos i watched .the color converted from yellow to purple and here is become orange why?
@kosheeka
@kosheeka Месяц назад
It depends on the dye you are using for your cell cytotoxicity assay.
@lailatarar5885
@lailatarar5885 4 года назад
Hi julia can you tell me in detail about PFC assay mean to say how to perform it
@CellGS
@CellGS 4 года назад
Hi Laila, The protocol for Orangu can be found in the following user guide: cellgs.e2ecdn.co.uk/Downloads/Orangu_User_Guide.pdf Please do not hesitate to contact us at tech@cellgs.com if you have any further questions. Many thanks.
@dr.l.v.prasaddiagnostics6035
@dr.l.v.prasaddiagnostics6035 2 года назад
WHAT IS THE DIFFRENCE BETWEEN Cell proliferation and cytotoxicity assay
@michaeljones2781
@michaeljones2781 2 года назад
Hi Dr Prasad. A cell proliferation assay measures any increase in the number of cells. A cytotoxicity assay measures the capacity of cytotoxic compounds to cause cell death, so markers of cell death are measured.
@kosheeka
@kosheeka Месяц назад
Cell proliferation assays measure cell growth, while cytotoxicity assays assess cell death caused by drugs or other factors. They provide insights into cell health and function, but target different aspects: growth vs. survival.
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