Chromatin immunoprecipitation (ChIP) explained: principle and procedure | ChIP assay

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This video lecture describes
1. What is chromatin ?
2. What is chromatin immunoprecipitation?
3. When and why Chromatin immunoprecipitation is performed?
4. What are the different steps involved in Chromatin Immunoprecipitation (ChIP) ?
5. How ChIP analysis is performed ?
6. What is Chromatin Immunoprecipitation protocol?
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Опубликовано:

1 окт 2024

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Комментарии : 45

@alessiailas4929 2 года назад

Thank you😭 that's the best explanation I found among all the RU-vid videos on the subject

@BiologyLectures 2 года назад

You are most welcome.

@سارهعادل-م6ع 2 года назад

this is a fantastic lecture. i hope to do another for plant bioinformatic and biostatistics as must, i am from egypt

@BiologyLectures 2 года назад

Thank you very much. Please consider subscribing us. For bioinformatics lectures, please go through this playlist ru-vid.com/group/PLi1EfMhc7RNr0XMmUCkKZWItx-TvBg0cY

@alhaeri1 2 года назад

thank you

@BiologyLectures 2 года назад

You are most welcome.

@mohammadgufran9560 3 года назад

Nice explanation

@BiologyLectures 3 года назад

Please share the video and subscribe our channel for more contents like this. Thank you.

@achanyasjayan9195 3 года назад

How can we protect chromatin protein from the agents which is used to remove cytosolic proteins in cell lysis stage.

@BiologyLectures 3 года назад

Crosslinking help fix protein DNA complex Chromatin) stable. If sonication is used in lysis, keep the chromatin on ice and limit the pulse to no more than 30 seconds to avoid protein denaturation due to heat.

@achanyasjayan9195 3 года назад

@@BiologyLectures thankyou❤

@BiologyLectures 3 года назад

@@achanyasjayan9195 You are most welcome

@dsdcd-x5t Год назад

thx🥰

@BiologyLectures Год назад

you are most welcome

@zer.e.zehranaqvi6439 Год назад

how we can analyze in the last step that DNA and proteins are interacting or not ?

@zer.e.zehranaqvi6439 Год назад

plz explain the last step in detailed

@BiologyLectures Год назад

We use the antibody specific for the protein in a DNA protein complex. And finally, we can analyze the data by performing qPCR. If we observe the Ct values for the gene of interest, that means that DNA and protein are interacting. We can also analyze by performing sequencing.

@zer.e.zehranaqvi6439 Год назад

@@BiologyLectures Thank you

@BiologyLectures Год назад

@@zer.e.zehranaqvi6439 You are most welcome.

@acampinglamp 2 года назад

Thanks for posting!

@BiologyLectures 2 года назад

You are most welcome

@investincrypto2859 Год назад

Very well explained about chip. Thanks a million

@BiologyLectures Год назад

Glad it was helpful!

@Hari788 4 года назад

Excellent explanation. Thank you 😊

@BiologyLectures 4 года назад

You are welcome!

@dikshyapanthi7681 4 года назад

WOW best explanation 😊👍

@BiologyLectures 4 года назад

Thanks a lot 😊

@shawlineema7431 3 года назад

extraordinary video!!

@BiologyLectures 3 года назад

Thank you very much. Please consider subscribing us for more contents like this.

@anujvermabiotech 2 года назад

Thanks

@BiologyLectures 2 года назад

Welcome

@linaabdelghany4033 3 года назад

Thank you for your explanation. May I ask a question 1- After the fixation step then use lysis buffer but how can we isolate the protein DNA complex from the cytoplasmic protein ? Do we use isolation kit or centrifuge? Thanks

@BiologyLectures 3 года назад

Since DNA is present in the nucleus, the protein DNA interaction described in the video is also nuclear. In the method, described in the video, we will not have protein DNA complex in the cytoplasm. Hope this helps.

@linaabdelghany4033 3 года назад

@@BiologyLectures so as in the method the DNA is the one that will purified and analyzed in the end of the process, it should be fine if the antibody bind to other proteins ( non DNA bound). Thank you for your clarification :)

@BiologyLectures 3 года назад

@@linaabdelghany4033 We use Chip grade antibody specific for the protein of interest. Lets say that if we want to study the interaction between protein X and the transcription factor Y. The Chip grade antibody used will be specific for protein X. If there is interaction between the protein of interest X transcription factors A, this complex will be immunoprecipitated by the specific antibody used. Its not at all fine if the antibody is not specific. There are two major considerations when choosing an antibody for ChIP-whether it will work in ChIP and whether it is specific. Ideally, the target will have an antibody that has already been shown to work in ChIP or in another immunoprecipitation (IP) application.

@linaabdelghany4033 3 года назад

@@BiologyLectures Thank you very much for your explanation.

@BiologyLectures 3 года назад

@@linaabdelghany4033 you are most welcome