This single-celled protozoan was imaged using a Zeiss Universal microscope fitted with a Nikon D4 camera set to 30 fps @ 1080p resolution. The cell membrane of the organism ruptures at about 0:40 but then appears to repair the damaged membrane and continue on, unaffected.
The objective was a 40x Zeiss Ph3 .8-1.0/na oil immersion planapo with iris. The photo-eyepiece was a Zeiss 12.5x wide field. The Universal's Optovar magnification changer was set to 1.25x (40 x 12.5 x 1.25 = 625x) The iris was stopped down to .8/na and the scope was setup for phase contrast viewing.
I've had many requests to explain what I mean when I refer to "darkfield-phase contrast lighting". With the objective iris of the 40x Zeiss Ph3 .8-1.0/na oel planapo with iris stopped down to .8/na and the scope aligned for phase contrast lighting the central dark area of the phase annuli in the condenser effectively functions as a darkfield stop and results in the black background while the alignment of the phase rings in the objective and condenser rings results in increased contrast common to traditional phase contrast lighting.
8 июн 2013
