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Clonogenic Cell Survival Assay (Colony Formation Assay) 

Henrik's Lab
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The Colony Formation Assay (CFA) or Clonogenic Assay is often used to determine which effects a specific drug or irradiation has on cells. This method is frequently used in cancer biology to investigate whether a treatment might be beneficial for tumor cells that harbour a distinct mutation.
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Time Codes: 0:00 Why to use the Clonogenic Assay 0:54 How to perform the Clonogenic Assay 4:22 Product Placement 4:59 Outro
#CFA #clonogenic #assay

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16 окт 2024

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Комментарии : 9   
@hanieazari4251
@hanieazari4251 3 месяца назад
really appreciate, great explantion
@abdelhamidmahmoud141
@abdelhamidmahmoud141 7 месяцев назад
How can i calculate the last graph (Survival fraction and concentration) ?
@quangvinhnguyen114
@quangvinhnguyen114 Год назад
I think the exact number is 5 ul in 5,995 ml medium right? (in 1:47) Thank you for the video by the way, it is very helpful for starters in scientific research like me
@christiangabrielfigueroaes5755
@christiangabrielfigueroaes5755 2 года назад
Would cells attach if they grow in suspension? I'm concerned most of my cells will die given the length of incubation time.
@henrikslab
@henrikslab 2 года назад
It depends on the cell line, check your cell line and the keyword clonogenic assay in the internet.. for many cell lines you would need to exchange medium in between more often!
@pausang2196
@pausang2196 11 месяцев назад
What about plating efficiency? PE
@Oculus_idealistae
@Oculus_idealistae 2 года назад
Thank you!
@TheAlialhammer
@TheAlialhammer Год назад
well explained 😀
@JH-mz4uz
@JH-mz4uz Год назад
great!!
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