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Compensation Deep Dive - 2/23/23 with Nicolas Loof 

FlowJo Media
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22 окт 2024

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Комментарии : 4   
@vblanche1
@vblanche1 Год назад
Nicolas, many thanks for this webinar. One question: when using a 10 colors "traditional" instrument, and running a 5colors panel, is it better to 1) acquire with all PMTs opened and use spectral compensation or 2) close unused PMTs and go for traditional or Autospill compensation? Thanks, Vincent
@NicolasLoof
@NicolasLoof Год назад
Hi Vincent! Based on our testing (presented in this video), using option 1 (acquire with all PMTs opened and use spectral compensation) will give you less spread of your data than option 2 (using non spectral comp). So I you have spreading issue with your data that cannot be resolved with better panel design, I will recommend you to acquire your data with all PMTs opened and use spectral compensation. Best
@vblanche1
@vblanche1 Год назад
@@NicolasLoof Dear Nicolas, thanks for your reply.
@vblanche1
@vblanche1 Год назад
Hello again, here at ru-vid.com/video/%D0%B2%D0%B8%D0%B4%D0%B5%D0%BE-5_AB-qVEZqs.html, for Positive, you use -for ex- "Size/APC-H7-A+", so you need to adjust the "APC-H7-A+" gate or is it ok to use "only" as positive the "Size" (or Clean up) gate? Thanks
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