Connecting Living Neurons to a Computer | Part 2 

maybe

Did I get "printed locally here in Quebec" right? :o

@@Alceste_ yes, the link to the screen print studio is in the infobox

*THE PART TWO WE HAVE ALL BEEN WAITING FOR* 🎉🎉🎉🎉🎉

Hi, i think the link isn't working

Bump and like for visibility

Sounds like you are an ideal buddy for this dude to keep track of details, if you didnt have a job already.

Up!

I was about to say that exact some thing. However, you left out the part about the goop in the gloopinator. You really need to check that too.

post doc in a university?

Somehow that doesn't seem that bad, it's a decent life purpose

That's more or less how I remember my high school years...

A fulfilling life

you know that there are theories that we live in a game. Soooo

That's a lot better of a purpose than to pass the butter.

Same as it ever was.

>wake up >your existence is this one >pass on thinking that was all there was to life

​@@poupeuu This is why I choose to participate in 5 dimensions

​@@jeremymcadam7400 rookie numbers, all of us in the 6th dimension are looking down on your struggles, purely for amusement I might add

Plato’s “The Cave” is Doom.

Bro is literally a classic evil scientist at this point

I cant stop laighting at that !

@@rubikscuber9277 Absolutely! (in the classical meaning of the word, like in "The Gay Falcon" (1941)) 😄

@@harriehausenman8623 sorry I mean to say Yay

@@spencerharrison9680 *tech company

Such is the cruel nature of life

Do not build the Torment Nexus. But if you do, do not torment the Torment Nexus with cayenne pepper flakes.

Sounds like a middle-age crisis

Almost human

every time i found one of his videos i get this harlan ellison vipes and i dont know why.

Instructions unclear, built torment nexus

Well it wasn't going to build itself.

@@ayybe7894 your not wrong lmao

Me: Wait, where does the "torment" come from in Torment Nexus? The Thought Emporium: Cayenne pepper flakes. Look at the neurons screaming in torment. Delightful. Me: Oh yeah, that makes total sense, thanks.

I'm just glad it's the humble mad scientist and not the techbros.

@@jakobweisser8044 "We built a neural interface on an unregistered cruise liner off the coast of Panama that mines cryptocurrency. We just need your donation of $200,000 to keep it running and to pay the illegal waste dumping fees." -techbro neuroscientists

More correctly: "Can it play DOOM?"

​@@ussuratoncachiCan it RUN doom? Can I use its brain as a computer to run doom?

Can it run in a Hamster Wheel to power the Platform running Doom?

The body is just the case for the computer

"👍✌☠ ✋❄ ☼🕆☠ 👎⚐⚐💣✍"

(*Cave Johnson enters the chat*)

@@johnpooky84PORTAL MENTION

Bro is a mad scientist 😭

Naturally. 🧐

I think he's not talking about a human brain (so far...).

@@samuelhomberg9075You want skynet? That’s how you get skynet.

​@@samuelhomberg9075give him time...

Researchers aren't nerds, they're just crazy

budget

@@mihael64 But... they are also usually nerds XD

@@mihael64 My advisor literally can not physically stop himself from talking about Dune if he drank more than 2 beers... And not every researcher is crazy. Some can even manage work life balance. Not anyone I know, but I heard some can!

The only difference between a researcher and a nerd is that researchers are paid.

I think that's because rats have less neurons to mature overrall. also because other animals (I think) gain consciousness faster

@@TheAmazingAnimator it is more about complexity of their connections and morphology and less about the overall number of cells. In culture you can observe the same time discrepancies between neurons of different species, so it is an inherent quality of the cells. We are not clear on what level of consciousness other animals have , since we can’t test for it. You probably mean animals become independent much earlier, since humans literally complete their development outside the mothers body. During evolution our heads got to big for our pelvises so we have to be born “prematurely”.

Thank you. We really appreciate all your input.

*losing in valorant* MY FRAMES! GET THE PEPPER

"Hey guys, my GPU is struggling to hit 120fps. Gotta go toss a 5 hour energy into the growth medium circulator."

Sprinkling it in like fish food into a brain in a tank

*playing fps game* *loosing connection* "Guys, my machine just had a seizure."

What i learnt is that injecting pepper into the brain will be an effective form of torture.

Rip And Tear, or R.A.T. for short.

Until it's done!

Man is gonna build a murder hungry superintelligence

Underrated 😂😂

Can it run doom?

That's the medium, not the algorithm.

Rotational Additional Tactics RAT 🐀🐀🐀 🐀. 🐀. 🐀. 🐀🐀🐀🐀🐀 🐀🐀🐀. 🐀🐀. 🐀 🐀. 🐀. 🐀🐀🐀. 🐀 🐀. 🐀. 🐀. 🐀. 🐀

and where do you work? A weapons corporation..

Power of anime

And that's why he is called Dr Stoned.

I loved that show! Hoping for a Season 3.

@@johnpooky84 I was wondering the same thing and I think there is a season 3 but it’s not on netflix yet?

@@Numbers-gStands Dr. Stone's on Netflix? I watched it on Crunchyroll.

Imagine just being born to not see, and then you just get random feelings all around you

the ultimate cheat system, full automation cheats; I could grind war thunder off a chunk of rat brain

​@@breadboi3837imagine getting banned for rat brain botting 😂

@@zachrowe6271 🤗

To be fair, you also have to think about, yknow, having a body and pressing keys and shit.

​@@user-burnerTrue that, all about focus, reaching flow state.

Feel free to send me an email with all of the suggestions so they don't get lost! And they're greatly appreciated as always. We only use phase contrast when checking on the cells. Fluorescence was only used at the end for the sake of the experiment, and the dye was only used on one of the arrays after we had finished all other experiments. The arrays are also only ever left on the microscope for as brief a moment as possible, but the phase contrast images aren't as pretty so I don't use that footage in the video as much. I also saw you recommend concentrating the neurons in the middle. I read that in the instructions and that's how we attempted it the first time when the cells died. We went with adding the neurons after the media because we knew for sure the neurons would survive as we've tested that method a lot and couldn't risk another culture dying. We were pressed for time to get the video out. And for our arrays the electrodes are REALLY spread out, so we're gonna need a high neuron density across most of the surface of the array anyway. You'll notice I made the walls of our arrays taller than the multichannel ones. It was specifically because I wanted to increase the amount of growth media per well so the neurons don't deplete it as quickly and to account for an eventual much higher neuron density across the array. We have wanted to upgrade our camera on the microscope for a while, but the scope itself was a HUGE upgrade. It's new for this video and it cost a pretty penny. But it's built exactly for this kind of experiment so we can switch out the camera next. I also want to move to a red dye to lower the illumination wavelength as you say. And also just to make the videos look more interesting than always green. We ran out of time, but we also got a tubulin specific dye we're gonna show off next time that would look best against an otherwise red stained neuron. And we picked up NucBlue so we can stain the nucleus properly. One of the shots of the neurons firing included it from the first well we tested it on. I overlaid it. It's subtle but makes the neurons look nicer and the nucleus visible.

Also, I've set your account to always show comments on the channel now, so they shouldn't get deleted.

​​@@thethoughtemporium youtube doesn't care and if the automated system even imagines your comment as bad it will just automatically delete it or hide it

New challenger appeared!

@@noi0124it’s the challenger space shuttle ! Ready? Set Fight!

Maybe they just have to chill… *checking notes* On no, wait. That was NOT the cure 😆

Too soon

Context? (Curious)

That was my favourite one!! 🤣

18:05 for those looking for the funny line

Wait I can’t tell if this is genuine or not

@@Penguinman2.0Neither can I… On a second reading, I don’t think it’s genuine, “I don’t remember what the moral of the story was” seems like it wouldn’t be there if it was.

@@Penguinman2.0 The book doesn't exist, its from a meme talking about how techbros proudly claim inspiration from scifi stories where the whole moral is NOT to build the technology featured in the movie. Like how vr/metaverse nerds constantly say "its like the Matrix in real life"

@@SissypheanCatboy or more recently, openai comparing gpt-4o to the movie "her"

​@@SissypheanCatboyThe first thing we did after GPT-1 showed promise was hook up the next version to the internet to learn from. It's like the people at the forefront of advancement have turned dystopian prophecies into a checklist..

The perfect combination of long-form content, diligent and passionate work, and humor!

Meanwhile RU-vid: Demonetized.

TestTube

@@yaysuu holy smokes you're onto something!

not only that, it would inspire young people from age 2 onwards to become youtubers and make the next big scientific discovery/invention!

For the ones randomly watching this and has no idea who Steve Potter is and why it is a big deal It is as if Jesus Christ himself showed up for a theology class

A truly impressive feat!

What a Legend.

I’m sure he’s never heard this joke, but he sounds like a wizard in this field.

@@akatsukilevi I can't not stop thinking about Professor brothers now lol

Hope the appointment went well! God bless!

What

I WAS THINKING THE SAME THING!

DMEM with FBS (foetal bovine serum) is an excellent medium for accelerated growth but yeah, it's 4x stronger than standard EMEM. EMEM or Opti-MEM may be a better option for maintaining mature cultures, with Opti having the added benefit of requiring much less FBS.

True. 😄

FINNNNALLLLLYYYYY

LETS GOOOOOOO💯💯

Ikr! I've been waiting for this for what seems like FOREVER!!!!!

yay

WE WILL FINALLY MAKE A MEAT COMPUTER

Which channel is this?

@@Astoria_Varanus Oops, I forgot to put his name: Travis McEnery. The series is called "The Spiders in Your House."

To be fair, if this project succeeds it will be the ultimate, unbeatable iteration of the running doom on X meme.

μ

i love mewing

Huh, it’s kind of comfy

Funny enough I have a mint in my mouth and was already moving my tongue

Thanks!

What have you cursed me with

Ever had your foot 'go to sleep' from holding it in an awkward position too long then walked on it?

Soon we shall have a flesh automaton animated by neurotransmitters!

Do not dare to insult the purity of steel by comparing mechs to abominable flesh

Bro just called God's greatest creation "neuron-piloted flesh mechs"

@@Mate_Antal_Zoltan It's a Cruelty Squad reference xP

@@audriusskebas3099 guy who has only played Cruelty Squad in his entire life: "I'm getting real Cruelty Squad vibes from this..."

And hopefully it won’t gain senti ence

Imagine it grows new connection base on the things you do on it, gradually speeding up.

The way that it will work is most likely they take the data from the game, asteroid for example, of an enemy being "to the left" and sends signal to a nerve cluster. Cluster then triggers, which tells the left cluster to turn left. Signal is then sent to a different sight cluster that says enemy is "in front" which tells the gun cluster to shoot. Just my guess, same way as us. We receive a signal from eyes that a wall is in front of us, so we don't run into the wall.

Shiz makes me question life every single time

They use signals to sense the obstacles, enemies, doors, walls, etc

NOT THE SPICEEEE AAAAA

Yeah, this could never go wrong, but to make sure lets wire a 100 of these up to the Boston dynamics robot wielding a shotgun. But we would still be in control, right? lol

I know this is a joke, but the interaction that the neurons see is not in any way related to violence. it's like if you played doom, but instead of a screen, you just see an array of 10 blinking lights and no-one told you that you're playing doom, only if you're succeeding at pressing the correct buttons or not. so ... as long as no-one tries to apply this to real-life weapons, there's no reason whatsoever to worry. though ... now that I think of it, knowing humans, this does indeed have me worried

​@@SafetyAlwaysLast Boston dynamics V1

Context- For it to work like the joke intended it would need some reason for violence like a reward system but for that to work we have to have some sort of detection software supporting it and at that point just use software, so yeah the joke falls apart, oh well. Except for machine learning being applicable to organisms, i.e. stimuli training, so maybe we could consider culturing them with positive stimuli for shooting the target before sending them but that sounds like a lot of work. Not that someone wouldn't try? Also if the logic reply wasn't to the Boston Dynamics bot joke then that is really embarrassing lol

@@SafetyAlwaysLast on the other hand, the neurons not being aware of it being violence points to another issue, since that means they won't have any concerns whatsoever with performing violence if put in a situation where they might accidentally do so. putting any kind of neural network, artificial or biological, in charge of dangerous equipment means that unless you very actively train it to avoid harming humans, it will do so if any humans get in the way. this is of course already the case with any kind of automated machines, even without neural networks or similar, which is why we tend to place robots in cages, so we can better protect humans from them

Yep, once it touches a new source, tips go bye bye. I'm surprised at some of the stuff done here, as we ALWAYS filter our media... And I haven't seen that. Also, just pouring tubes out can be a source of contamination.

Wasn't there the old riddle about what the glitter companys biggest customer was and they have absolute secrecy and nobody knows? 🤔

​@@harriehausenman8623 Yes, and it is most likely pearlescent paint for hobby boats. Boat manufacturers don't want it to be associated with how "unmanly" glitter is seen as, as well as the fact that pretty much all of it ends up as pollution in water.

The wires look like they might be fiber optic? Apart from USB, but that’s error corrected

It'll end ul learning to Google for pictures of cheese and female rats.

​@@carlpanzram7081 and cat slander.

only as much as you'll freak out when you realize there's a fourth dimension you can't rotate yourself in

@@rootabeta9015 Did somebody say 4D Golf? 😄

@@rootabeta9015actually I’m rotated in time by 4°

@@rootabeta9015 sounds like a skill issue, I can rotate in the 4th dim just fine

​@@harriehausenman8623 non-intuitive double rotation intensifies*

I can't remember today. It is currently happening. I can't remember tomorrow. It hasn't happened yet. Soon I will forget about yesterday.

​@@MaxBrix bro forgor 💀

ikr

How many todays are there? There’s no need to shout.

@uriituw ok boomer

COMPRENTION

spelling is beyond your own comprehension

Thank you!

Yes!

@@thethoughtemporiumwhich one?

Too late buddy 😂

Hey what was the unedited?

Adding capsaicin to the culture was the start of the villain arc

Wow, this comment is hilarious and I really appreciate the originality😐

@@Bread78787 shh-

the devs take a while to update the game

Horton Conway would approve this comment. 🙏 R.I.P.

In the words of 4 year old me, "I like the spicy chemical."

It did!