Тёмный

Counting Cells with ImageJ 

Kevin Foley
Подписаться 411
Просмотров 408 тыс.
50% 1

Keep in mind that ImageJ processing is not perfect. However, the error of missing cells may be "corrected" by the error of including some noise.
Steps in Video:
Process - Subtract Background
Image - Adjust - Threshold
Process - Binary - Fill Holes
Process - Binary - Convert to Mask
Process - Binary - Watershed
Analyze - Analyze Particles
Basic description of tools:
Subtract Background - cancel out noise of the background
Threshold - change the image to binary image of red, black and white, or blue
Fill Holes - fill empty spaces between rings to make circles
Convert to Mask - allows for subsequent processing
Watershed - automated separation of separate fused cells by a 1 pixel line
Analyze Particles - process the image to acquire a cell count
Size - parameter of what cells to include in data by area (pixels^2)
Circularity - parameter of what cells to include by how close to a circle the shape appears. The maximum bound (1.0) means a perfect circle. The minimum bound deviates from being a circle.
Show - Outlines will trace the outside of the mask images. Masks will show the black and white image similar during thresholding.
Exclude Edges - ImageJ will not include cells that are not fully contained in the boundaries of the image
Macro Code:
run("Subtract Background...", "rolling=12");
setAutoThreshold("Default dark");
setThreshold(35, 255);
run("Convert to Mask");
run("Fill Holes");
run("Convert to Mask");
run("Watershed");
run("Analyze Particles...", "size=120-Infinity circularity=0.00-1.00 show=Outlines display exclude clear summarize");

Опубликовано:

 

17 окт 2024

Поделиться:

Ссылка:

Скачать:

Готовим ссылку...

Добавить в:

Мой плейлист
Посмотреть позже
Комментарии : 75   
Далее
moto tag - AirTag для Android
00:47
Просмотров 428 тыс.
Wildest 10 SECONDS OF HIS LIFE 🤯 @TomIsted
00:14
Просмотров 4,8 млн
3D Gaussian Splatting! - Computerphile
17:40
Просмотров 141 тыс.
How AI 'Understands' Images (CLIP) - Computerphile
18:05
Cell Counting in QuPath
22:34
Просмотров 1,9 тыс.
Жадные алгоритмы
11:10
Просмотров 7 тыс.
Cracking Enigma in 2021 - Computerphile
21:20
Просмотров 2,5 млн
Solving Wordle using information theory
30:38
Просмотров 10 млн
Counting Cells with a Hemocytometer
6:31
Просмотров 1,3 млн
Counts in ImageJ
26:53
Просмотров 7 тыс.