Day 5 Embryo Biopsy 

It’s amazing that you’re able to watch this video. Have a nice day!

Thank You! Both are Hamilton Thorne

We use a standard ovoil, but we do customize our pipettes for better control.

@@reproductivegeneticinnovat8725 How do you customize your pipette ? Can you please share ?

We extend the tip to elbow length and we do this in-house with specialized equipment.

The hole can be 5-10 µm. Yes, freezing immediately after biopsy is acceptable.

We use custom pipettes with a longer tip to elbow distance for added control

At RGI we try to use the edge of drop for most embryo biopsies. This approach allows us to make the cytoplasmic bridge between the sample and embryo as thin as possible. That, in turn, allows us to minimize the number of laser pulses to 1-2 or avoid them completely. To stretch the cytoplasmic bridge to its maximal we do both: pulling with holding pipette and pulling by the biopsy pipette simultaneously. We also use flicking, but in rare cases, when for some reason a laser cannot completely detach the biopsy sample from trophectoderm.

In this video, assisted hatching was performed on day 4. Hatching can also be performed on the day of the biopsy on day 5/6.

Do you perform biopsy on blastocists on day-7 or you just discard after the 6th day of colture?

We do not discard potential embryos on day 6 and will preform day 7 biopsy on good quality embryos only.

Day 5

No, we use the edge of the drop specifically to prevent the embryo from being pulled out of the zona.

@@reproductivegeneticinnovat8725 I tried your method but the drop did not cut off the cell, it just make my embryo become into strip shape.

In this video, we used a single laser pulse (400 micro seconds @ 100% power) that resulted in the cells separation from the trophectoderm. Without laser assistance we would not be able to remove the cells. The edge of the drop is used only to stretch and thin out the bridge between the trophectoderm and biopsy cells with allows us to minimize the number of laser pulses.

The drop is approximately 5-15 µL covered with oil (ivf)

We like to have 3-5 cells.

@@reproductivegeneticinnovat8725 how many mosaics PGT-A diagnoses do you have on total? Do you transfer them after genetics counselling?

Per PGDIS guidelines, mosaic transfer recommendations are deferred to the provider. In our PGT lab, where we work with clinics from around the world, mosaics account for about 8% of PGT-A diagnoses.

Only about five cells are removed from the outer layer of the embryo. This layer will eventually become the placenta and therefore should not affect the development of the embryo. This technique is necessary for PGT.

@@reproductivegeneticinnovat8725 very informative 👍