After entering all the settings, when pressing "Start". I was getting an error: "path or file name not found". Reinstalling the program solved the problem
This is super cool. I'm a bit of a novice to these types of scientific image processing. For improving diffraction limited pictures on the PUMA microscope, would you prefer to use blind deconvolution? I'm guessing it might also be possible to try to use the spatial light modulator for SR-SIM or to determine the PSF using microparticles.
Thank you. I did a lot of work on super-resolution in bright field microscopy years ago and I hope to bring some of that to this channel over time. Yes - I hope also to demonstrate some SIM with PUMA - but there are a several more videos on the theory side I want to get out first so everyone can join the party.
I have installed windows version and I try the example step by step according to your instructions, however when I press 'start' then after a few moments I get the following error: a biaqlm header file cannot be found for the selected array/image. Do you have any suggestion what could be the problem?
Thanks for the comment,. Clearly I can't know for sure but here are some possibilities / suggestions: * One or more of the programs may not have installed correctly. Try a re-start of your computer. If that does not fix it, try uninstall and re-install +/- restart. Try typing the commands 'deconvolve' and 'fitsdou' (without any arguments) on a command window and see if they are recognised (you will get the standard usage message if it is recognised). * It may be that some antivirus software has decided to block or quarantine some of the programs so you might need to look into that and re-install once permissions have been granted / files un-quarantined. * All BIPS programs cannot handle any file name or directory path that contains spaces - so if you installed the program to something like 'My Programs' or if any of your files are in folders like 'My Document' (or anything with a space anywhere along the full path) then this could cause some programs to fail to find files and run. * Check any feedback messages on the console window - these might give you a clue as to where the problem occurred or even what it is. If you are using the standard demo without alteration you should not require any additional dlls (if you tick the 'Use parallel' box then certain multithreading dlls will be required). If you manage to get it working, consider making a reply to this comment to state what the problem was and how you fixed it to help others who have a similar issue. Thanks,
