Denaturing protein samples for SDS-PAGE or western blotting 

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In this tutorial video I am showing you how I prepare my lysate samples once I've completed the BCA assay to determine total protein concentration in my samples. I will go over my calculations and how I prepare total sample protein (ug)/(uL) total sample volume. For denaturing my samples I make use of 4X Laemmli and 90C heat.
Time stamps
0:00 Introduction
0:54 Zoomed in 4x Laemmli instructions
1:08 Mixing Laemmli components
2:37 Calculations from my BCA assay protein concentration results
4:28 Recapitulate calculations for [50ug/25uL]
5:42 Calculations for second sample [40ug/25uL]
7:32 Preparing first lysate protein sample with 4X Laemmli
9:19 Preparing second lysate sample with 4X Laemmli
11:40 Vortex and quick spin of samples
12:00 Denaturing at 90C for 5 min