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DNA Microarray (DNA chip) technique 

Henrik's Lab
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Hey Friends,
DNA Microarrays cover a lot of tasks such as gene expression analysis and genotyping. How this DNA chip technique works, is explained in this animation.
Best,
Henrik
Time Codes:
0:00 Introduction: Why to use a DNA microarray
0:33 Sample preparation
1:15 DNA Microarray chip - Mechanism of Action
3:01 In the lab

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19 июн 2024

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Комментарии : 105   
@henrikslab
@henrikslab 7 месяцев назад
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@jadon5798
@jadon5798 5 месяцев назад
geiler typ man hört den akzent aber du sprichst deutlich und das was du sagst ist sehr verständlich und top erklärt vielen Dank du hast mir den arsch gerettet
@ii.noorii
@ii.noorii 10 дней назад
One of the best videos to explain the DNA microarray, thank you❤❤
@monkey6493
@monkey6493 Год назад
Better job than my teacher ever could, thanks a lot.
@roshan3190
@roshan3190 11 месяцев назад
Exactly 💯😂
@V_MA
@V_MA 2 года назад
Great! I needed to watch a video about DNA microarray to understand how it works. It helped me a lot! Thank you.
@simafarokhnejad5882
@simafarokhnejad5882 5 месяцев назад
This video is one of the best explanations of DNA Microarray I've come across on RU-vid. It's concise and efficient, and it is clear even for someone without a background in biology.
@sahanahn19
@sahanahn19 2 года назад
It would have been very helpful if you could upload the notes of this microarray.. Explanation is amazing sir!!
@bhavik3201
@bhavik3201 7 месяцев назад
absolutely outstanding explanation. Thank you. Love from india
@Maria-jl5is
@Maria-jl5is Год назад
that was brief and so useful, thank you so much
@pitkamatka
@pitkamatka 2 года назад
Hi Henrik! I just discovered your channel and I think your videos are of great help for me. Thanks for making them :)
@ma.8805
@ma.8805 2 года назад
you are the best, thank you
@warwar5380
@warwar5380 8 месяцев назад
thank for making complicate topic easier to understand
@shiweanyswami7371
@shiweanyswami7371 Год назад
Thank you it just blessing to me cleared everything thank u so much please upload more vedios am enjoying to study from here and my concepts get more strong cause you made it clear thanks onece again
@muffinfighter3680
@muffinfighter3680 Год назад
thanks! Now, I understand how this analysis works
@JulianTheHunter
@JulianTheHunter 2 года назад
So simple erklärt, herrlich! Vielen Dank
@eloisub902
@eloisub902 2 года назад
Really straightforward! TYSM
@papridas8979
@papridas8979 2 года назад
Thank you🙏 very clear explaination!
@khadeejazardawy971
@khadeejazardawy971 2 года назад
thank you so much you made it easy to understand
@yuqingcai3447
@yuqingcai3447 2 года назад
This is very helpful! Thank you!
@sameloliveira6169
@sameloliveira6169 6 месяцев назад
Great explanation, thank you very much
@user-ch7xw4ed8u
@user-ch7xw4ed8u 2 месяца назад
Amazing! Best one on RU-vid! Thanks
@callmebonobononim
@callmebonobononim Год назад
You are amazing!! Now I can understand finally🎉🎉 thank you soooo much Have a nice day😆😆
@thefayadbrothers1170
@thefayadbrothers1170 2 года назад
Very clear thank you!
@Mrzaharna
@Mrzaharna 2 года назад
Actually it helped me a lot to understand , thanks a lot
@m2-instruments
@m2-instruments Год назад
Great video, very well explained 👍🏼
@tina9753
@tina9753 11 месяцев назад
Very helpful video:) thanks!
@narthakiyasendra2917
@narthakiyasendra2917 2 года назад
Amazing job
@itstimetostudy397
@itstimetostudy397 Год назад
Very nice, thanks 👍
@melikaabdorrahimi3768
@melikaabdorrahimi3768 2 года назад
Amazing!
@bh5169
@bh5169 2 года назад
Thank you!
@graemelaubach3106
@graemelaubach3106 2 года назад
Awesome channel, keep it up!!!
@chickensandos
@chickensandos Месяц назад
Great video man
@halimish
@halimish 4 месяца назад
that was really helpful thank you⭐️
@ebircsbus6643
@ebircsbus6643 10 месяцев назад
I can hear your german accent a mile away lmao This was a amazing explanation bro thx for putting in so much work!
@harekrishnamandal1428
@harekrishnamandal1428 2 года назад
Highly conceptional
@tatianaasi3315
@tatianaasi3315 2 года назад
Very great explanation, im studying for med school entry
@afnanashraf5
@afnanashraf5 6 месяцев назад
Great!
@user-sz5it4wm9f
@user-sz5it4wm9f 2 года назад
A good explanation 👍👍
@robin2385
@robin2385 2 года назад
well explained video, the best i habe found, but i have a question: so the oligonucleotides must be complementary to the cDNA and the cDNA has the same direcetion as the leading DNA (3' 5'), so the oligonucleotides have the complementary direction of 5' 3'? sorry for the bad english
@user-yehia
@user-yehia 8 месяцев назад
Great explanation
@mohammedal-hammadi5085
@mohammedal-hammadi5085 2 года назад
Thank you
@syedmohammadaanasfarukh890
@syedmohammadaanasfarukh890 2 года назад
Great stuff
@dieterandrich1014
@dieterandrich1014 2 года назад
very nice very simple
@AM-hp7ht
@AM-hp7ht Год назад
Great , Good job 😍😍😍🤩🥰
@panosfragalas3161
@panosfragalas3161 Год назад
Thank you alot
@SajidAli-zm8fc
@SajidAli-zm8fc 2 года назад
Really nice
@anjalipatel4107
@anjalipatel4107 5 месяцев назад
Very nice explanation sir😊
@suriyaprakash2252
@suriyaprakash2252 10 месяцев назад
This is very helpful.,! Thank you! I want to know about which Bioinformatics tool has been used To (Raw)data analysis.,
@mariociencia12
@mariociencia12 2 года назад
It is incredible your ability to do concise videos with a lot of information and completely animated! I have just a question: how exactly does the cDNA become fluorescent? All the bases used are fluorescent?
@henrikslab
@henrikslab 2 года назад
Thanks, that means a lot! I did some more in depth research... and there are many ways in practice. One as you mentioned is using fluorescent nucleotides (all dCTPs are labeled). Another option is: using fluorescent forward primers. This paper might help in case you are super interested in the labeling steps: www.sciencedirect.com/science/article/abs/pii/S0167701205001806
@henrikslab
@henrikslab 2 года назад
Another possibility: Using 3´end labeling with fluorescent nucleotides + terminal transferase (www.future-science.com/doi/10.2144/000112182)
@binebum1
@binebum1 2 года назад
Super interesting and clear. Wish you could do some videos on the energy metabolism of cancer cells!
@henrikslab
@henrikslab 2 года назад
@@binebum1 I did one about the warburg effect.. check that out!
@user-ef3uq9qe9f
@user-ef3uq9qe9f 6 месяцев назад
Nice and easy
@prachitrivedi5538
@prachitrivedi5538 Год назад
good explanation
@doaaismail11
@doaaismail11 2 года назад
this is very clear than the dr. explanation
@sebastianomoscolin267
@sebastianomoscolin267 7 месяцев назад
🔥🔥🔥
@lstrawaya6403
@lstrawaya6403 2 года назад
Merci
@henrikslab
@henrikslab Год назад
From now on you can become a member of this channel! You will have access to some cool emojis like this:. This is of course voluntary, but the financial support helps me a lot.
@a_9773
@a_9773 Год назад
Kuss geht raus, brudi
@sketchy1113
@sketchy1113 2 года назад
Very easy😁
@sandrawatt
@sandrawatt Год назад
Clear and concise explanation! Question: does each sample go on its own microarray, or are they mixed and applied on the same microarray? Thanks!
@henrikslab
@henrikslab Год назад
Each sample is typically applied to its own microarray.
@henrikslab
@henrikslab Год назад
However, there is also the possibility to apply multiple samples to a single microarray. This is commonly done with a technique called "multiplexing". It is done by hybridizing different labeled samples to the same microarray, or by using different probes for each sample on the microarray.
@sandrawatt
@sandrawatt Год назад
Ah, that's why it was confusing. It seemed like "sometimes". This really clarifies it. Hope it helps others too!
@caasinom8008
@caasinom8008 2 года назад
How does hybridization occur? Are the oligonucleotide probes single-strands as is the cDNA or is there a thermocycle similar to PCR?
@henrikslab
@henrikslab 2 года назад
Yes, the oligos are single stranded!
@maryjakr8538
@maryjakr8538 4 месяца назад
Why would the sample 2 material not binding to the array cDNA mean that the gene is not expressed? Could it not just mean, that there is a mutation (e.g. microduplication) that prohibits the probe from binding while the gene can still be translated and expressed into a protein by the cell in a „loss/gain/change of function“-mutation?
@anmolpardeshi3138
@anmolpardeshi3138 Год назад
the health and disease sample complimentary strands that is put in one tube is from the same person or one is from the case and other is from the control?
@henrikslab
@henrikslab Год назад
Technically, that depends on the research question you are asking, both would be possible
@Unreal2424
@Unreal2424 2 года назад
Hi, I got few questions 1 - we agree that we label AFTER retrotranscription during samble preparation? 2 - The oligonucleotides found in each microarray, are complementary to cDNA ? so they are DNA and not RNA ?
@henrikslab
@henrikslab 2 года назад
Hi, 1 - there might be other assays, but mostly you label with fluorescent nucleotides DURING cDNA synthesis 2 - Yes, they are DNA
@Unreal2424
@Unreal2424 2 года назад
@@henrikslab thank you !
@ahmetziyaaktas9179
@ahmetziyaaktas9179 10 месяцев назад
How the cDNAs hybridize with the probes. Probes are one-stranded okay, but aren’t the cDNAs are double-stranded?
@bts_rollercosterlifepurplearmy
@bts_rollercosterlifepurplearmy 6 месяцев назад
U save my 7 marks 😀
@muhadsaghaffar746
@muhadsaghaffar746 Год назад
👍
@effiek3886
@effiek3886 Год назад
Can we have more samples at the same time;Does this technique only work for cancerous cells or any type of unhealthy cells;
@henrikslab
@henrikslab Год назад
Yes, you can apply multiple samples to a microarray at the same time. This is commonly done using a technique called "multiplexing". Multiplexing allows multiple samples to be processed simultaneously on a single microarray. It is done by hybridizing different labeled samples to the same microarray, or by using different probes for each sample on the microarray.
@HenryGhost-dx9gb
@HenryGhost-dx9gb Год назад
how do u make ur animation
@henrikslab
@henrikslab Год назад
To 95% I create everything in Powerpoint, only rarely I also use Inkscape
@urfujji937
@urfujji937 5 месяцев назад
Thanks for concising it rather than many slides lol.
@evilrubin2455
@evilrubin2455 2 года назад
Ginge das auch auf Deutsch? :D Nicht sonderlich schwer rauszuhören. Mein ich nit böse, danke für's Video!
@oe4022
@oe4022 2 года назад
Thanks for this video ! just a small point here 0:45 "tissue from a healthy cell" doesn't make any sense, you should have said cell from healthy tissue or something like that.
@sara-qz8zw
@sara-qz8zw 7 месяцев назад
Is there anyone here to help me? . My master asked us to find (the mRNA -DNA ybridization use for which microbes?) Pleaseeee help me
@sara-qz8zw
@sara-qz8zw 7 месяцев назад
Hybridization
@user-xe4yu2om8j
@user-xe4yu2om8j Год назад
Is the oligonucleotide RNA or DNA?
@user-sz9ji9fo1e
@user-sz9ji9fo1e 28 дней назад
Rna only
@robin2385
@robin2385 2 года назад
let me guess, you are german, but nice vid
@PoojaKumari-jl4ly
@PoojaKumari-jl4ly Год назад
From 0:40
@dernachname5240
@dernachname5240 Год назад
das ist ein sehr deutsches Englisch haha. ist aber gut erklärt danke :)
@ym.hj_
@ym.hj_ Год назад
Vote park gunwook
@hannahx6347
@hannahx6347 Год назад
you are German, aren't you?
@sameerhussain3709
@sameerhussain3709 Год назад
He is Indian
@ChigaChad
@ChigaChad 9 месяцев назад
Sound indian
@goharabrar4191
@goharabrar4191 8 месяцев назад
It's literally in the Chanel description mate🤦🤦
@ldngroulette2888
@ldngroulette2888 6 месяцев назад
Ja er hat einen deutschen Akzent, und jetzt?
@adamnogell7663
@adamnogell7663 23 дня назад
The guys in the replies probably never heard an indian accent lol
@mmmmmmmmm4383
@mmmmmmmmm4383 4 месяца назад
😢
@Destina-du9sl
@Destina-du9sl Год назад
Du bist doch Deutscher, dann füg doch wenigstens Untertitel ein🥲
@945395jagan
@945395jagan Год назад
How do u apply this technique in microbiology to detect pathogenic microbes?
@Mary-eu6bm
@Mary-eu6bm 2 года назад
Thank you!
@navadeepdorai2918
@navadeepdorai2918 Год назад
Thank you
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