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Droplet Digital™ PCR Tips & Tricks: ddPCR™ Assay Design 

Bio-Rad Laboratories
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The QX200™ Droplet Digital PCR (ddPCR™) System, Bio-Rad's second-generation digital PCR system, provides absolute quantification of target DNA or RNA molecules using EvaGreen or TaqMan hydrolysis probes, yielding unmatched sensitivity and precision for a wide variety of applications.
The webinar discusses tips and tricks used in ddPCR assay design. Topics covered include the availability of commercial PrimePCR™ ddPCR assays from Bio-Rad, common pitfalls, and recommendations for designing an effective ddPCR assay.
Presenter: Dianna Maar, Senior Scientist, Bio-Rad Digital Biology Center
Dr. Maar studied at the University of California Los Angeles, where she received both her Bachelor’s and Doctoral degrees majoring in Microbiology, Immunology, and Molecular Genetics. During her graduate training, Dianna studied antibiotic resistance, ribosomal mechanisms, and protein expression. She continued to work on translation and protein expression at The Scripps Research Institute. She expanded her research interests at Sandia National Laboratories where she worked on developing bio-fuels from protein fractions, identified virus-entry mechanisms and gene expression profiles for Nipah Virus.
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9 июл 2024

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@huirusun1448
@huirusun1448 Год назад
Hi, this vedio helps a lot when I get to know the ddPCR technology. I have been reading the material "Droplet Digital PCR Application Guide", and I noticed in sample preparation part, we need to "digest in the lowest possible volume and mix with water before adding the digested DNA to the reaction mix." Is it mean that the sample need a pre digestion before adding to the reaction mix. I am wondering whether a pre digestion of the DNA sample is needed. Or I can just add the enzyme to the reaction mix with no need to incubate as shown in the slides.
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