We used to use these type of microscopes on air flotation tables. They were a bugger to get dialed in but it would allow them to be used vibration free.
If that microscope was rigid from cam to lens to the specimen table, I don't think it would wobble because - it looks like the cam is moving separate from the wafer -. I'd be checking for play in the cam or specimen table.
Hexane is what we were always told to use to clean lenses back when I was in lab, we were told to use this because its virtually non polar and because the carbon-hydrogen bond in special in that it doesn't interact with other materials (something about how its liquid state is maintained almost entirely via van der waals forces), where other commonly used solvents like xylene may be non-polar, but they contain aromatic rings which interact with other materials.
excellent education video by David and Dave -audio input . got to love the LED light bodge Job. it's a hobbyist classic is to avoid any one off pcb fabrication and just hot glue it. Hot Snot it! also it just goes to show why, a good consumer 3D printing service is useful for that one off 3D printing job. no everyone needs a 3D printer at home.
In relation to the shacking, if you clamp the sample to the solid microscope stage you'll reduce most of that. Also, in medical research we often have our better microscopes sitting on a 250-750kg stone table top and sometimes these tables will have mechanical dampers built into the legs.
For the fairness of all involved you should start adding freeze-audio overlays over your own videos too! As a replacement for the stupid removal of annotations...
The trick with hex keys is that you need a metric and a imperial set ;) I used to work as a sound and light tech and we mostly used JBL speakers. You could tell if the speaker was made in USA when it had imperial hex screws :D
i think he mentioned in the other video when he was using the microscope that the other desk he had it on was screwed down and very solid and yet still saw vibrations
Cotton will contain microscopic minerals, that will scratch the glass. I look at cellulose all the time in a SEM, at work. Natural materials are more contaminated.
Hey! Any chance we can get a link to the 3d printer files for the adapter you've done up? I just got one of these scopes myself and the bulb is missing so I figured I'd attempt your mod! Thanks.
Hi Dave2, Lova ya so far, but I recommend you watch Crocodile Dundee about 30 times in a row. That way you can learn to play up the 'Strailianess we all love!
The lm317 will drop over 3V in constant current mode. Since the LED needs 3V, this will not regulate with just a 5V power supply. LED gets no where near 300mA.
Exactly. Scanned down the comments expecting lots of people to have raised this but they don't seem to have. A standard LM317 needs between 1.5 and 2V to maintain regulation at 300mA depending of temperature. Add to that the 1.25V dropped across the current sense resistor and it's 2.75 to 3.25V. Looks like David needs a bit more practical electronics experience.
Easiest way to check if the the final circuit is regulating is to check the voltage drop over the resistor. It wont be 1.25V if the power supply is too low.
The speeded up video bugged me a bit, I don't mind a bit of waffling. Re, the "dirty" inside of top tube thingy, David's obviously never mucked pigs out or changed a nappy; that's dirty. Not bad for a 1st video David, you need to slow down a bit though. No worries, I know how hard it is to relax when filming yourself.
Is that David's first official channel one video at all? There was the one with the 3D-printer, right? One thing that came to my mind while I was watching this video was: While you're at hacking the light, wouldn't it be possible to add a mount for lasers, so you could use the scope to measure thicknesses of layers by interferometry? Would such an addition be helpful for analysing ICs or anything? I know it's a whacky idea, but I guess it would be possible.
Nice vid Dave 2. would like to see more of this kind of videos. BTW which was the video where Dave confronted Dave 2 the first time with that evil power supply? Would like to watch it again. Must have been 2 years ago or so?
Hi Dave 2. Interesting first official video! Tiny bit of advice, maybe you could talk a hair slower and pronounce a hair better. That's all. Just one little thing. Such a fine piece of gear. Does that really deserves only just a botched together current source? :(
never clean lenses with a dry cloth, no matter how soft it is. there are lots of small hard particles in that dust that will scratch the lens when you move them around. the only way to do it is with something wet.
Christopher Stone I got mine in one of my local Hardware Stores (33 Drawers/Unit for 8.99€). The shipping costs from China will ruin the price advantage you get.
Looks like David needs a bit more practical electronics experience. A standard LM317 needs between 1.5 and 2V to maintain regulation at 300mA depending of temperature. Adding the 1.25V dropped across the current sense resistor takes that to 2.75 to 3.25V, leaving a 1.75V max load voltage drop in order to maintain regulation on a 5V supply. Measuring the current into a short presented by an ammeter isn't going to show the current into the LED dropping 3V.
Hopefully your not trolling and want an actual response. This was a two birds with one stone solution, if inadequate current (less than 300 mA) achieved sufficient brightness then job done. If not power from an alternate source and get the full 300mA. It was only a few minutes of work for a solution for both scenarios. I probably should have kept more footage, not very good at editing at the moment and I wasn't sure what to keep. In both scenarios the LED is safe from over current. Device is temperature stable at 300mA, building is aircon'd + heatsink sufficent. If your curious its running at about 50% relative luminous flux (166mA) what it could, at 300mA it would be at about 80%. We didn't yet need the extra brightness, might later, we will see. I know that the multimeter doesn't include forward voltage of LED or set drop, this is pretty basic and ultimately it didn't matter. It showed that I didn't pick the wrong resistor and that it wasn't open circuit or shorted, it also showed that the LM317 was functional as many were second hand. The test meant that I could be sure that I was indeed protecting the LED from over current for both scenarios. Thanks for the feedback, would you prefer longer videos with the extra details that fully justify a design decision or more like a project summery?
Why on earth do you think I'm trolling? You made a schoolboy error and I pointed it out. Trying to justify the error does you no favours. That's "you're" in the third para BTW.
tohopes would be very funny if dave2 did the same thing to dave1 if he got to editing as well. Thumbs up to dave2 being a permanant host. Though saying questioningly Power supply of doom? is there video of why this power supply earned the nickname?
yep, there is a video, gotta go back 6-8 months at least I just remember it being about power supplies, possibly a review, and then this came in on a tangent, it was about UI simplicity
I appreciate your response but its no problem, it was my idea for him to correct me. Its an Aussie joke, Aussies obsessively will correct you if you pronounce Z "incorrectly". It reckon it was a fun edit :)
I liked the video. I also didn´t mind Dave's voice over. Don´t get me wrong, it wasn´t necessary - but it added even more value. Two heads are better than one.
You'll have to adjust the camera! Dave II is a bit taller than the average Auzzy Oompa-Loompa. :P Nice vid, future is looking good for one of the best channels on RU-vid.
Dude! Do you know how lucky you are to be picked by "The Great Dave Jones" to be his "apprentice"? Quit thinking "I'm recording what are people gonna think.................?" in the back of your mind That's killing you! Get your confidence back! Speak your mind! Don't worry what we think! We are watching to find out what you think! LOL Quit pausing,studdering,second guessing yourself. I'm not sure but i think the master has said "GROW A PAIR" Man you have a great opportunity to do great things! Relax stop being nervous and speak your mind....... best wishes..............
I understand the confusion, but in this case it is correct, Z is the 3rd dimension. On the ground, map or piece of paper (or the table from the microscope), the dimensions are X and Y, the Z is the height above it.
david aka dave2, has been working with dave for over a year on and off while also doing university which he recently finished. dave bought an old microscope, it needed cleaning, some people do care. mailbag = hoard :P