ELISA Analysis in Excel with 4PL 

Hello! Thank you for your comment. I'll be happy to assist further with your data analysis. Could you please send an email to support@myassays.com with your kit insert or data analysis instructions?

@@myassays Ok Thank You

Hi, Thank you for your comment! Lower R² values can be indicative of a poor fit. In these instances, it is recommended to review the assay procedure and obtained data for the standards. Check the standards follow the expected trend for the assay. If everything is as expected, it may be that a different fit method should be used. To easily evaluate different fit methods, you may find our free curve fitting tools available at www.MyCurveFit.com to be helpful. If you would like additional assistance specific to your data, please send your assay and run information to support@myassays.com.

Hi Reguinaldo! You can download a free trial of the MyCurveFit Excel Add-In at the following link to begin analyzing 4PL and 5PL data directly within Excel: www.myassays.com/download-and-install-mycurvefit-excel-add-in.html I will be happy to assist with creating an Excel template to analyze your data, please email support@myassays.com with your assay requirements. Thanks!

Hello! I'll be happy to assist, with your analysis. Please send an email to support@myassays.com to allow further discussion of your requirements!

Hi Fernanda Decaro! Yes, the MyCurveFit Excel Add-In can be used to perform analysis for competitive ELISA, and the template available for download will provide a great starting point. Please email us at support@myassays.com and we will be happy to assist further with creating a spreadsheet specific to your analysis.

Hi! The ELISA spreadsheet template is a fantastic starting point for analyzing ELISA data. You can update this template to suit any plate layout or number of standards included in your assay. If you'd like a demonstration, or an updated example spreadsheet, please send us an email at support@myassays.com with additional information about your analysis requirements and we'll be happy to assist!

Hi! Thank you for your message. To allow me to get a little additional information and assist further with troubleshooting, please email us directly at support@myassays.com. Thanks!

Hello, thank you for your message. To calculate the dilution factor, use the formula: Dilution Factor = (Final Volume)/(Initial Sample Volume) So for example if you've diluted a sample 1 part to 4 parts diluent, you might use 100µL sample and 400µL diluent. Using the above formula: Dilution Factor = (100µL+400µL)/(100µL) Dilution Factor = 500µL/100µL Dilution Factor = 5 If you have any additional questions, don't hesitate to reach out via email: support@myassays.com. Thanks!

Hi! The MyCurveFit Excel Add-In can be used to perform analysis for a wide range of assays, to allow me to assist further with your specific application, please provide the kit insert or detailed data analysis instructions to support@myassays.com. Thanks!

Hi, thank you for your comment. I'll be happy to provide further support via email or a brief GoToMeeting to help you get started. Please email us at support@myassays.com to allow me to assist. Thanks!