_Thank You Teacher For the Wonderful Session_ ❤️❤️❤️🙏 _Love From India (Kerala)_ 🥰💖 _This Video is Helpful For Kerala Class 12 Botany Practical Exam_ 🥰💯❤️⚡
I've always loved plant biology when I was a child, sadly (but probably for the better) that love eventually got pushed out by an interest in technology, however, now my (eighteen years younger) little sister got a microscope for Christmas and guess who's up at 4am to get everything ready for looking at cells? Yeah, it's me, and you're helping me out really well, thanks a lot! :)
I hope to make a video of that soon. In the meantime, here is a verbal explanation for how I approach this. Roots are generally too thin and soft to section in the way we do stems; therefore, place the root on the slide and, using a fresh blade (like the one shown in the video), draw the blade across the root as though you were slicing a warm loaf of bread. You will not be pushing the blade down but instead will be slicing across the root (same motion as you do for the stem). I hope this helps.
Thank you so much! Would you mind if i ask a one question? I bought a TBO to dye my plant, but it was in the form of powder. I'm curious what i should do to use it.
Here is my video showing how to prepare TBO Stain (and iodine potassium iodide); the written instructions are below the video. ru-vid.com/video/%D0%B2%D0%B8%D0%B4%D0%B5%D0%BE-F96E-8iRNII.html
The plant shown in this video has both glandular and non-glandular trichomes. The glandular trichomes have a spherical cell at the top that can contain various compounds depending on the species. Both types of sections (longitudinal and cross) make visible the distribution of tissues within the plant stem but they differ relative to the "long axis" of the plant. The cross section is taken across (or perpendicular to) the long axis, and the longitudinal section is taken along (or parallel to) the long axis. Both types of sections are valuable, and both are required for a full understanding of the anatomy of the plant organ.
Hello can you tell me please, can Isopropyl 9.99% be used instead of ethanol to rinse plant specimens after staining? and if so what percentage of water to Isopropyl should I use, or shoud I use Isopropyl in its purest form? Please excuse my ignorance, I am only just starting out with Microscopy, so at the moment I know very little.
I'm happy to say that it isn't as scary as it might look, and (hopefully not jinxing anything) no students have been injured in the preparation of these sections. The movement with the blade is very small and controlled. I'm always impressed by how good the students' slides turn out! (I'm the Beyond the Bean Seed creator)
It should work just fine. Give it a try and post your results here! I've only had difficulties with stem cross-sections when they are from tissues that have lots of sclerenchyma fibers or are otherwise very tough. In those cases, I use essentially the same technique except that instead of holding the stem section in my hand and drawing the blade *towards* me, I place the stem to be sectioned on a cutting surface and slice downwards (like slicing fresh-baked bread). It is crucial to use a fresh blade (the blades I introduce in Episode 2, Red Bell Pepper) and to wet the blade and stem before cutting.
Wetting them helps ensure good contact and helps the blade glide while making the cut. Also, the film of water will "collect" the thin sections of plant material, which will adhere to the blade as the material is removed from the main section of plant material. I think it is good to keep the plant material hydrated throughout the process as much as possible to minimize damage and the inclusion of excess air bubbles.
For compound microscopy, you need samples to be thin so that light can pass easily through it. If sections are too thick, light will not be able to pass through and you won't be able to see details of the cells or tissues.
kaufen sie sich doch mal ein gebrauchtes Ultramikrotom und machen Glasmesser. Die kosten etwa 300 Euro und vergessen sie diese Spielzeuge. microtome toys
