Estimation of Serum Albumin by BCG Method || Bromo Cresol Green Method || Biochemistry Practical 

Estimation of Serum Albumin by Bromo Cresol Green (BCG) Method - This is the video on estimation of serum albumin by BCG with demonstration and discussion of viva questions.
it contains
Introduction to Albumin - 00:40 minutes
Normal Ranges of Plasma Proteins - 01:32 minutes
Functions of Albumin - 02:28 minutes
Maintenance of Colloidal Osmotic Pressure by Albumin - 03:43 minutes
Principle of BCG method - 04:02 minutes
Operational Procedure - 04:49 minutes
Calculation of Serum Albumin - 05:40 minutes
Determination of A/G ratio - 06:25 minutes
Normal ranges of Albumin and A/G ratio - 07:06 minutes
Causes of Hypoalbuminemia - 07:55 minutes
Causes of Hyperalbuminemia - 08:32 minutes
A/G ratio - 09:17 minutes
Discussion of Viva Questions - 10:23 minutes
Demonstration of Estimation of Serum Albumin by BCG Method - 12:05 minutes
Albumin, globulins and fibrinogens are the major proteins present in blood / plasma.
Alb is major constituent (60%) of plasma protein with conc. 3.5-5.0 g/dl.
It is synthesized by hepatocytes; therefore estimation of albumin is a liver function test.
Half-life of albumin is about 20 days.
Plasma protein = albumin + globulin + fibrinogen
Serum= plasma (alb + glb) - fibrinogen
Total Protein=6.5-8.5 g/dl
S.Albumin =3.5-5 g/dl
S.Globulin =2.5-3.5 g/dl
Fibrinogen =200-400 mg/dl
A/G Ratio =1.2:1 to 2.5:1
Functions of Albumin
1. Maintenance of colloidal osmotic pressure.
2. Role in transport-act as carrier for hydrophobic substance
(bilirubin, non-esterified FA, drugs(sulfa,aspirin,dicoumarol), hormones(steroid,thyroxine), metals(copper,calcium & heavy metals) etc)
3. Maintenance of acid base balance
(buffering action of protein-alb has 16 histidine residue which contribute to buffering action)
4.Nutritional function
( complete protein as it contain all essential AA, serve as AA sources & contribute to protein synthesis during period of starvation)
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