Expression vectors | What is in an expression vector? | applications of expression vectors 

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@@animatedbiologywitharpan sure I will

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please share among friends and help me to reach big audiance

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Please share my channel link with your friends and help me to reach big audience .

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Share among your friends and help me to reach big audience

Please share my channel link with your friends and help me to reach big audience

Please share my channel link with your friends

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Aysha Ayshaa please share among your friends

@@animatedbiologywitharpan sure , I will ✌

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Of course.....keep it up..plz

Both eukaryotic and prokaryotic genes can be expressed using the expression vectors but the type of expression vector is different.

Most of them are shuttle vectors such that can be used in prokaryotes And eukaryotes at the same time... hence they have two origin...further watch my videos on shuttle vector

yeahhh i just found your video on shuttle vector, thank youuu

Fizha Adnan good

Very interesting question, this should be a video on it's own. In short, Linkers and adaptors can be used which are oligonucleotides that are useful in DNA ligation during blunt end cloning. In cloning experiments the cleaved DNA often has blunt or sticky ends. Sticky ends are easy to ligate but DNA strands with blunt ends are hard to ligate. The linker and adaptor molecules then come into play. The linker and adapter molecules have both blunt and sticky ends and internal restriction sites as well, which help in DNA ligation.

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so naturally our insert of interest itself has sticky ends and how do we know which sticky end Bam, Hind or which has?

Rna seq or if you have candidates then you can do qPCR

That’s a gateway cloning vector .... actually gateway cloning is much better than the restriction ligation based approach.... efficiency is much higher

@@animatedbiologywitharpan thanks. Can we directly insert our gene of interest or do we need to use pentr D TOPO

It's artificially provided in the vector because it helps recruit the ribosome to the mRNA to initiate protein synthesis by aligning the ribosome with the start codon. Goal of an expression vector is to express the protein in bacteria/ eukaryotic cell.

I create everything in PowerPoint

You have to clone the promoter sequence along with your target gene and then put it in the vector. In general expression vector already has promoter sequence

@@animatedbiologywitharpan yes so.. I need to remove that promoter ? And if yes so there is no restriction site, in both side of promoter so how?

The most popular ones are generally circular. There could be some linear phage based vectors but i dont know much about them

Thank you

I mean I can't hear sometimes clearly