Extracting Plasmid DNA: How To Do a Miniprep

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In this method video, Molly takes us into the lab to teach us how to purify plasmid DNA from a liquid culture of bacterial cells. When purifying plasmid DNA from a small volume (3 to 5 ml) of bacterial cells as we do in this video, this technique is called a miniprep.
View this video (and more like it) on LabXchange: www.labxchange.org/

Опубликовано:

26 июн 2022

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Комментарии : 65

@samsonkamau5048 9 месяцев назад

I am not used to commenting but this one deserves a thumb's up. excellent explanation with clear and precise illustrations

@user-zd7xv7wc2t 6 месяцев назад

This video is one of the best teaching for learning extraction plasmid.

@bullymag Год назад

This work deserves way more than that amount of view. Excellent work. Liked and subscribed!

@birdo54 Год назад

This is a FANTASTIC video! Thank you very much, this was extremely well done.

@LabXchange Год назад

Glad you enjoyed it!

@shirinbehnamsani2366 Год назад

The first time I understood what I was doing this whole time in the lab. Thanks for the amazing explanation!

@mateuspaiva5916 Год назад

The material in the video is amazing, and the scientist is also an amazing actress! Great job in here, keep up with your inspiring work.

@MichaelSmith-ij2ut 5 месяцев назад

Hours of lecture and explanation distilled in to 15 minutes.

@gapat8213 Месяц назад

What an incredible science humans has developed. This is something to be grateful for everyday. Plasmid will have the potential to cure all diseases when the special interests will allow it.

@isaacco2876 Год назад

this is a great video! thanks for the info!

@KatayonZare-uq9cm Год назад

That was PERFECT thank you for your explanation❤

@harp6198 Год назад

Thank you very much! This was excellent!

@pingsart7402 6 месяцев назад

wonderful demo

@nurmuhammad4540 Год назад

Excellent, thank you so much!!

@supermunheeb Год назад

Gonna do this tomorrow this helps me a lot! Thanks.

@UnKnownVlogger01 4 месяца назад

Every student like me deserve an advisor like you Mam 😅 Very well explained

@brilllance8903 8 месяцев назад

perfect video. clear narration. overall 10/10

@michaelokeke7783 4 месяца назад

Amazing! I love every bit of this video; so refreshing!

@srobles1660 9 месяцев назад

EXCELLENT WORK!!!

@reconeix 9 месяцев назад

Excellent, detailed, and approachable presentation. You're a natural educator!!!

@btssketch937 Год назад

Thanku so much for this amazing video I really appreciate your efforts ❤❤

@haleemasadia5225 8 месяцев назад

Thanks a lot Mam for such well illustration! It helped me a lot as I'm a student of MS biochemistry

@christiankemakolam7028 6 месяцев назад

protocol simplified and made interesting. kudos

@WeDream18 Год назад

Amazing explanation...❤ It covers each and everything you need to know. loves the way she explain.

@sebastiantraxler5550 4 месяца назад

Amazing video!

@natalia8414 Год назад

Great video!😊

@parisahouri 3 месяца назад

The best! Thanks a lot 😊

@blacktear5388 2 месяца назад

This was super helpful, thank you so much

@isabelasoares1168 Год назад

Thank you for this amazing video!!! This channel will help me a lot

@shubhamaryan2972 8 месяцев назад

great explanation...

@03500 5 месяцев назад

It was amazing, can’t thank you enough 🙏🏻🙏🏻

@mobinahsansiddiqui 22 дня назад

Excellent Explanation, concise, and informative. Thanks.

@hana-al 5 месяцев назад

It was excellent !! thank you ❤️

@aamnasajid6570 5 месяцев назад

the best video I ever saw

@rufinamenezes7712 5 месяцев назад

An amazing and fun way of explaining. Thank you very much. Please continue it so that many may benefit from your explanations

@DiemNguyen-gg8gy 9 месяцев назад

Thank you very much for the excellent video! It is very helpful!❤

@alsaqar1 Год назад

Best video in this subject 🎉🎉

@salmanashraf8790 Год назад

Super method of explaining lady (teacher)

@user-lp5qe1tv7d 4 месяца назад

Loved it thanks😊

@UdhayaBalaKanna111 5 месяцев назад

Just amazing!

@maxmordente2218 Год назад

Thank you!

@Brume20 7 месяцев назад

Amazing video.

@ayeshakhan279 Год назад

Thank You!

@Omar-Khaairy Год назад

Thanks so much

@minahil21 8 месяцев назад

Very well explained mam

@md.1276 2 месяца назад

Don't get me wrong but I was here for science but stayed till the end for her smile

@taylormarkel1094 8 месяцев назад

thank you so much!!!!!

@shreeramesh9902 6 месяцев назад

awesome

@kadircanvural6814 Год назад

awesome! thank a lot

@arianevitoriasilvestrehann5140 5 месяцев назад

I loved it!

@alhaeri1 3 месяца назад

legend

@Ganesh_lamichhane Год назад

How beautiful your video like your smile. I liked your video and your smile. Keep smiling and make videos.

@christopherleubner6633 24 дня назад

Very informative. Highly recommended for molecular bio and genetics students. ❤ ill take my plasmids chilled served neat. 😂

@omkarmore7613 2 месяца назад

Very amazing with a very pretty mam

@wednesdaysfrog8422 9 месяцев назад

Which kit have you used?

@emmanuellaamara1876 15 дней назад

what kit did you use, I have been trying plasmid extraction for months now, but I haven't gotten a reasonable result

@ghazalehgolmohammadnezhadk5307 Месяц назад

What happens if I forgot to wait for 1 minute before the last centrifuge?☹️

@rogersundeen4022 9 месяцев назад

Everybody got this? It's going to be on the test.

@lauraschonborn5633 Месяц назад

You are so sweet and cool 🎉

@richardlange7559 6 месяцев назад

Some of that equipment is filthy!

@bebolovesshrek 16 дней назад

How

@badangel842 Год назад

I little bit confuse for Explanation O_o

@juxyclips671 9 месяцев назад

Whats her @?

@ThatStickyNote Год назад

Please do not inoculate your bacteria by poking a pipet tip by hand. Hold the sterile pipet tip with sterile tweezers (hold the tweezers in a blue flame for a sec) and then poke the colony on the agar. Also work under a flame or in a lateral flow cabinet.

@LabXchange Год назад

Thanks for the feedback! In this video, Molly is working with media that includes selective antibiotic. If she were working without antibiotics, she would have used sterile tweezers. It's important to note that best practices can vary, so you should always check with your PI if you have questions.