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Fitting for Saturation Binding 

Jin Wang
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In TR-FRET studies, saturation binding is the first step to measure protein and tracer binding affinity. It is highly recommended to measure both total and non-specific binding (without the receptor protein). This video explains how to use GraphPad Prism to analyze the saturation binding data.

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11 сен 2024

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Комментарии : 3   
@TheFeralligator
@TheFeralligator 3 года назад
Hello, thank you for this video. I had one question: in the case of doing a semi log XY graph of log10 ligand concentration on the X axis and specific binding on the Y axis, how can I interpret the data on the graph by finding the Bmax value by seeing where the max X value intercepts on the Y axis, and from this calculating the Kd constant through looking at the 50% occupancy value?? Thank you!
@JinWang876
@JinWang876 3 года назад
I am not sure whether semi log is the right way to plot the data. It is unlike the Hill equation to calculate EC50.
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