Flow Basics 2.5: Instrument Setup and Automated Compensation 

series of high quality presentations, I have been doing cytometry for 15 years, this will allow me to explain with precision what I was doing without sometimes knowing why

Bravo!!!!

Thank you for an excellent course. I am new to flow cytometry - how do you ensure that a single stained control / compensation control is brighter than overall sample, if the cell population we are targeting (in a compensation control tube) has low expression of surface marker? I would assume that a fluorophore-conjugated low expression surface marker would not emit high fluorescence signal due to small number of cells expressing said marker, even if the cell suspension is stained with a bright dye such as PE. Thank you for your help.