Gel Electrophoresis 

Ok

😂😂😂😂

r u taking both biochem and medicinal chem? how r u?

Jesus shaves, hallelujah! 🙌🏻😎

Went from Professor Dave to Professor Daddy overnight. Kinda looks like a buffer penguinz0

Common misconception… Anions are negative, and cations are positive. However, in gel electrophoresis, the cathode is negatively charged, while the anode is positively charged. (BIOLOGY) You are, I believe, referring to a galvanic cell. In a galvanic cell, anodes are negative and cathodes are positive. (PHYSICS)

Nothing switched, it’s just that there are different perspectives in the two subjects (physics and biology). The SURFACE of an anode is positive and the SURFACE of a cathode is NEGATIVE. However, the CORE of the anode is negative and the CORE of the cathode is positive.

us too

Because there's an electric charge applied, the anode is the positively charged electrode :)

Its called the anode because its where anions move, its unrelated to the charge, it would make sense to be cathode as its cationic but its where anions move so we call it an anode, similarly cations move to the cathode

That's what I do best😉

Tysm

Ok I will

You can extract DNA/RNA from a cut-out section of an agarose gel by dissolving it.

One of my techniques😉

3:05 & 3:15. The left hand lane contains DNA of fixed sizes to use as a scale. In checking correct insertion you can run a lane with the unaltered plasmid as a comparison or (better) digest some of the new plasmid and check to see if the right size insert appears.

Even though it has bigger negative charge it will have more molecular weight.

Because the negative charges are evenly spaced along the DNA backbone, and the number of charges are proportional to the length, the DNA receives a very even force per unit length. As a result, the migration is affected mainly by the difficulty in sieving through the gel pores. Where the charge of the molecule has a big effect is in Native-PAGE protein electrophoresis, since a heavily charged protein will be pulled through faster. In SDS-PAGE (the first picture Dave showed with the bright blue sample wells was SDS-PAGE) the protein is solvated by negatively charged detergent, so it acts mainly on size.

Nope, the positive anode attracts anions toward it, therefore it is a positively charged electrode :)

ty

That’s very true, any objections to that?

Non-denaturing PAGE.

What’s the difference between them?

EtBr will (with UV) but bromophenol blue is just a marker dye that tells you the gel is running OK.

Np

haha

The videos are on RU-vid, bud. If you subscribe and click the bell for notifications, it will tell you when I upload.

I think you confused anion with anode.

No cathode is negative and anode is positive. Exactly the opposite charge of what cations and anions are