In this tutorial, I will explain how to perform gene set enrichment analysis on your differential gene expression analysis results. We will use the R package fgsea() and you will learn how to:
- Install and start fgsea()
- Prepare your dataset to perform GSEA
- Set the analysis parameters and run the analysis.
- View the GSEA results and get some nice plots!
And as always, you can find the code I am using in this tutorial at biostatsquid.com, where you can also find a step by step explanation of the code. For this tutorial you will need R, or Rstudio, and you will need to install the fgsea() package.
Hope you like it!
biostatsquid.c...
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Other interesting resources for pathway enrichment analysis:
Other interesting resources for GSEA:
Main GSEA webpage: www.gsea-msigd...
More on the method itself: www.pnas.org/d...
Paper comparing pathway enrichment analysis methods: www.nature.com...
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20 авг 2024