Absolutely brilliant!! I understand the process far better now, you should do more videos on DNA replication processes and cellular respiration etc. You have a natural gift teaching with sound scientific skill behind your methods.
This was AMAZING! Your explanations was crystal clear (and really creative) and it was really neat seeing you do the same thing in the lab. Looking forward to more!
Nice video!! Great idea to explain with the draws! Just for the record... sometimes the background music was louder than your voice, so it got hard to listen and focus at these points. Thank you!
Crap i kept watching to vid because of how pretty she is then ended up becoming educated on how to isolate and copy a gene. It was done so well that even an idiot like me was able to understand it.
you are AMAZING!!! Really did the perfect job of explaining what I find, a very complicated process with such clear illustrations and explanations. I'm sitting for my pure biology national exam paper tomorrow and this video is a god-sent blessing. Great job :-)
I love it! It would have been helpful to show how you can run the restricted frog DNA and choose the appropriately-sized fragment for insertion into the plasmid, too.
Thank you so much! I am currently taking Biology 30 and the information that they were giving me in my lessons about genetically engineering recombinant DNA just wasn't making sense. You explained this concept so well, thank you!
The last step could have just been putting the bacteria into a fluid containing ampicillin. The ones that didn't take up the plasmids (containing the ampicillin resistance gene) would've simply been destroyed.
Your video is really interesting and explains things well. But l have a question that why don't first use gel electrophoresis to identify the frog gene instead of inserting all the gene into the bacteria and then cut it off and then identify? It seems more intuitive that we first find the right gene and then insert it into bacteria. And it may save bacteria😄. I am a year1 undergraduate student and can someone help me? Thank you very much!
Okay I'm a bio major but what you did seems a bit counter intuitive (please correct me if I'm wrong) You cut both frog Dna and Bacterial Plasmids Mixed them up and reassembled them with the help of Ligase then you INSERT the combined plasmids INTO bacteria, ONLY to EXTRACT them again for further analysis. Why would you insert the plasmids in the first place if you're just going to take them out for further analysis?
almost atheist They are reinserted back into the bacteria so that the bacteria can reproduce exact copies of the new bacteria, all containing the Frog DNA. Thus creating a Frog DNA "Factory". From a few modified bacteria cells you get many.
The initial insert of the frog DNA into the E. coli was to use the bacteria's replication machinery to mass produce the DNA since bacteria grows a lot faster. But as she mentioned, when the frog's DNA fragment is taken up by the plasmid you don't know which fragment is inserted. So to see if the desired DNA was inserted she take the [grown] E. coli and cut their DNA to be loaded on the gel along with the plasmids. The gel electrophoresis will separate the DNA depending on their fragments. Since the desired DNA fragment is larger than the undesired, you can visibly distinguish the fragments after running the electrophoresis. They can then just cut out the gel containing that band and use it to do what they want with it. At least, this is what I got from her video & from my lab lectures.
Aren't the plasmids ampicillin resistant other than tetracycline resistant? Also, how are different plasmids distinguished from each other? What does the gel electrophoresis do?
Hmm if you cut the same purple piece out of the plasmid, doesn't that mean that it was pointless to put them into the in the bacteria first place? It glosses over how the gell box works, as I would of liked to see how that worked :) Other than it's a great explanation of the other steps.
