Henrik, your content always makes my day. Your videos are always so professional, interesting and informative. The amazing graphics make science so entertaining... Thank you very much, keep them coming 🔬
By treatment with alcohol the outer membrane of gram negative removed and the crystal violet+iodine bond washed out. As gram positive have thick peptidoglycan layer so this bond of (crystal violet+iodine) is not washed out.. So retained. after that when these both are treated with safranin ( counterstain have almost pink color) the gram negative adopt pink colour and gram positive remain purple due to thick peptidoglycan layer. It is differential staining procedure
Thanks for this detailed information........ I've seen many videos on RU-vid related to this topic........but it's nearly impossible to find someone, giving the proper mechanism like you did.........majority described that gram positive bacteria cannot take the counterstain, they don't even explain the reason for the decolourisation. But you cleared a lots of confusion.........so thanks again 😊
The outer membrane is not removed; the crystal violet/iodine is washed out with alcohol in gram negatives due to their much thinner peptidoglycan wall. Gram + retain the violet color.