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Graphical Estimation of Kd from P:L Binding Plot

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Опубликовано:

21 авг 2024

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Комментарии : 20

@santanamendoza4279 7 лет назад

I'm very surprised that this video doesn't have more views! By far the best explanation of these graphs I have seen thus far. Thank you!!!

@Silent_Sounds 10 месяцев назад

you are so much better at explaining these concepts than my tenured professor at a nationally recognized research institution. Props and thanks for helping me pass biochem.

@tendekaim 5 лет назад

omg you save lives! you have no idea...shortest and best explanation ever thank you

@OrthoMetaParanoid 7 лет назад

Brilliant video, thanks!

@kalkidanberhanu7708 3 года назад

This is just amazing! Thanks :)

@AmitKSingh-pk3ll 5 лет назад

very nice explanation

@muhammadkhattak6922 7 лет назад

Thanks a lot for the video, you are great at it.This helped me very much with my school stuff. Keep up the good work.

@umamaheshwari1878 6 лет назад

+Catalyst University good

@thatboyss5576 3 года назад

Would this not give the initial concentration instead of Kd. We would then use this initial concentration to solve for Kd using the equilibrium equation.

@ching-hung-yao 6 лет назад

very good video help me a lot

@shushmagudla3920 6 лет назад

So are Km and Kd similar? Km also substrate concentration at 0.5 Vmax. How are the two different?

@SohanurRahman09 5 лет назад

Nice video...Thank you very much.

@CatalystUniversity 5 лет назад

Thank you!

@curtis4994 Год назад

you're fantastic

@emilyh6442 6 лет назад

So a higher Ka would also mean tighter binding/higher affinity? Thank you

@animeandstuff5377 4 года назад

no problem

@atawahperry1852 6 лет назад

thanks was useful

@ummeamara5942 Год назад

how to get y-axis value?

@busoladaramola9616 6 лет назад

why cant it be at 100% theta, completely saturated ?

@robertluxenhofer1579 5 лет назад

Not using 100% theta has a simple practical reason. As the curve inevitable slowly, asymptotically approaches 100% the 100% is hard to determine experimentally. The 50% value, however, is much more simple to determine.