H&E Staining 

A lot goes into this. I wonder how someone figured out the alcohol concentration quantities and times needed.

the alcohol you're referring is due to "XYLENE removal" process just after deparrafinization...Xylene is miscible w/ absolute alcohol..so use 100% alcohol to remove xylene...then descending concentrations of alcohol..from 90-50% will add-up water from the tissue..."90"% alcohol means there is 10% water in it, 50% alcohol mean 50% alcohol and 50% water . That is where the tissue becomes hydrated..ready for staining...hope u get it.

I'm just being started. Let this video be my reference point! Wish me good luck!

@DerSchnurrbart You sure don't. We use an automatic staining machine that dips slides in xylene for about 5 minutes per bath. The gradient of alcohols they use is much more long and involved than ours too. It might have something to do with the specific formulation of the stain they used, but I don't know. This seems more complicated than it has to be.

How much time is minimally required to prepare H and E slide for reporting?

This was...AMAZING!!!! Thanks for the vid!

Thanks, this helped me with my university assignment

@TheRealHarsjan for "Dehydration" alcohol starts from lower concentration to higher then to absolute concentration. Rationale: if tissues are submerged directly to high alcohol con. tissues will become hard or brittle, the outer portion of the tissue will dehydrated quick but the inner part is not fully dehydrated. Prior in performing H & E staining, you need to " Deparaffinize " use xylene, then remove xylene by absolute alcohol ( xylene is only miscible w/ absolute alcohol )

Why the serial dilution in organic solvents?

why that guy didnt differentiate or blue?

latest video : H&E Staining | Purpose of staining in histopathology | Human Touch in H&E Staining : ru-vid.com/video/%D0%B2%D0%B8%D0%B4%D0%B5%D0%BE-WBUriohMkSM.html

what is the meaning of two changes of 2 mins each? it just try which put from PBS to absolute EtOH twice?

@DerSchnurrbart yes i agree.

I don't think you need to put the slide into the xylene so long when you are deparaffinizing it.

is this Harris’s haematoxylin ?

Thankyou so much sir

I know that the alcohol is used for dehydration, but why so many different types of concentration? (student :) )

Thanku so much

Sir thankyou apke video se bahot help ho jati samjne main ese hi agge hamare liye video banate rhe

Soo helpful thankss

That procedure is too long,and I've notice in this video that no diferentiation with 0.5% HCL after hematoxylin was done.

1 hour in Xylene is a lot isn't it? 0_o We only do 6 minutes total in Florida.

@TheRealHarsjan after removing xylene...you need remove the alcohol from the tissue and replace it with water..so that your stains will have more affinity to the tissue. how do you do this? by passing through series of high concentrations of alcohol ( starts from your absolute to remove xylene) then to 90-80-70 then finally to water...then you can start H& E staining.

Fantastic And easier

Hello, what are the most widely used fixative in HandE staining method A. Formaldehyde B.zenker formol C. Picric acid D. Osmic acid Please help me answer

saaai tá tudo errado

5 min in xylene is all u need to get rid of the paraffin and just a min each in the other reagents

@MrJazkiller oo ok thanks very much indeed :-)

Is this how babies are made

Hello, what are the most widely used fixative in HandE staining method A. Formaldehyde B.zenker formol C. Picric acid D. Osmic acid Please help me answer